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SORTING NEXIN1 Is Required for Modulating the Trafficking and Stability of the Arabidopsis IRON-REGULATED TRANSPORTER1
Dicotyledonous plants growing under limited iron availability initiate a response resulting in the solubilization, reduction, and uptake of soil iron. The protein factors responsible for these steps are transmembrane proteins, suggesting that the intracellular trafficking machinery may be involved i...
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| Published in: | The Plant cell 2014-03, Vol.26 (3), p.1294-1307 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c412t-8b69fd919d9f390db1d3731f8fd349c67955d20696078623a3853e697b45193f3 |
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| cites | cdi_FETCH-LOGICAL-c412t-8b69fd919d9f390db1d3731f8fd349c67955d20696078623a3853e697b45193f3 |
| container_end_page | 1307 |
| container_issue | 3 |
| container_start_page | 1294 |
| container_title | The Plant cell |
| container_volume | 26 |
| creator | Ivanov, Rumen Brumbarova, Tzvetina Blum, Ailisa Jantke, Anna-Maria Fink-Straube, Claudia Bauer, Petra |
| description | Dicotyledonous plants growing under limited iron availability initiate a response resulting in the solubilization, reduction, and uptake of soil iron. The protein factors responsible for these steps are transmembrane proteins, suggesting that the intracellular trafficking machinery may be involved in iron acquisition. In search for components involved in the regulation of Arabidopsis thaliana iron deficiency responses, we identified the members of the SORTING NEXIN (SNX) protein family. SNX loss-of-function plants display enhanced susceptibility to iron deficiency in comparison to the wild type. The absence of SNX led to reduced iron import efficiency into the root. SNX1 showed partial colocalization with the principal root iron importer IRON-REGULATED TRANSPORTER1 (IRT1). In SNX loss-of-function plants, IRT1 protein levels were decreased compared with the wild type due to enhanced IRT1 degradation. This resulted in diminished amounts of the IRT1 protein at the plasma membrane, snx mutants exhibited enhanced iron deficiency responses compared with the wild type, presumably due to the lower iron uptake through IRT1. Our results reveal a role of SNX1 for the correct trafficking of IRT1 and, thus, for modulating the activity of the iron uptake machinery. |
| doi_str_mv | 10.1105/tpc.113.116244 |
| format | article |
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The protein factors responsible for these steps are transmembrane proteins, suggesting that the intracellular trafficking machinery may be involved in iron acquisition. In search for components involved in the regulation of Arabidopsis thaliana iron deficiency responses, we identified the members of the SORTING NEXIN (SNX) protein family. SNX loss-of-function plants display enhanced susceptibility to iron deficiency in comparison to the wild type. The absence of SNX led to reduced iron import efficiency into the root. SNX1 showed partial colocalization with the principal root iron importer IRON-REGULATED TRANSPORTER1 (IRT1). In SNX loss-of-function plants, IRT1 protein levels were decreased compared with the wild type due to enhanced IRT1 degradation. This resulted in diminished amounts of the IRT1 protein at the plasma membrane, snx mutants exhibited enhanced iron deficiency responses compared with the wild type, presumably due to the lower iron uptake through IRT1. Our results reveal a role of SNX1 for the correct trafficking of IRT1 and, thus, for modulating the activity of the iron uptake machinery.</description><identifier>ISSN: 1040-4651</identifier><identifier>EISSN: 1532-298X</identifier><identifier>DOI: 10.1105/tpc.113.116244</identifier><identifier>PMID: 24596241</identifier><language>eng</language><publisher>United States: American Society of Plant Biologists</publisher><subject>Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis Proteins - physiology ; Cation Transport Proteins - metabolism ; Endosomes ; Gene expression regulation ; Gene Expression Regulation, Plant ; Genes ; Iron ; Iron - metabolism ; Iron regulatory proteins ; Mutation ; Phenotypes ; Plant cells ; Plant roots ; Plants ; Protein Transport ; Sorting Nexins - genetics ; Sorting Nexins - physiology ; Statistical significance</subject><ispartof>The Plant cell, 2014-03, Vol.26 (3), p.1294-1307</ispartof><rights>2014 American Society of Plant Biologists</rights><rights>2014 American Society of Plant Biologists 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-8b69fd919d9f390db1d3731f8fd349c67955d20696078623a3853e697b45193f3</citedby><cites>FETCH-LOGICAL-c412t-8b69fd919d9f390db1d3731f8fd349c67955d20696078623a3853e697b45193f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/43190404$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/43190404$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,315,786,790,891,27957,27958,58593,58826</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24596241$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ivanov, Rumen</creatorcontrib><creatorcontrib>Brumbarova, Tzvetina</creatorcontrib><creatorcontrib>Blum, Ailisa</creatorcontrib><creatorcontrib>Jantke, Anna-Maria</creatorcontrib><creatorcontrib>Fink-Straube, Claudia</creatorcontrib><creatorcontrib>Bauer, Petra</creatorcontrib><title>SORTING NEXIN1 Is Required for Modulating the Trafficking and Stability of the Arabidopsis IRON-REGULATED TRANSPORTER1</title><title>The Plant cell</title><addtitle>Plant Cell</addtitle><description>Dicotyledonous plants growing under limited iron availability initiate a response resulting in the solubilization, reduction, and uptake of soil iron. The protein factors responsible for these steps are transmembrane proteins, suggesting that the intracellular trafficking machinery may be involved in iron acquisition. In search for components involved in the regulation of Arabidopsis thaliana iron deficiency responses, we identified the members of the SORTING NEXIN (SNX) protein family. SNX loss-of-function plants display enhanced susceptibility to iron deficiency in comparison to the wild type. The absence of SNX led to reduced iron import efficiency into the root. SNX1 showed partial colocalization with the principal root iron importer IRON-REGULATED TRANSPORTER1 (IRT1). In SNX loss-of-function plants, IRT1 protein levels were decreased compared with the wild type due to enhanced IRT1 degradation. This resulted in diminished amounts of the IRT1 protein at the plasma membrane, snx mutants exhibited enhanced iron deficiency responses compared with the wild type, presumably due to the lower iron uptake through IRT1. Our results reveal a role of SNX1 for the correct trafficking of IRT1 and, thus, for modulating the activity of the iron uptake machinery.</description><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis Proteins - physiology</subject><subject>Cation Transport Proteins - metabolism</subject><subject>Endosomes</subject><subject>Gene expression regulation</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Iron</subject><subject>Iron - metabolism</subject><subject>Iron regulatory proteins</subject><subject>Mutation</subject><subject>Phenotypes</subject><subject>Plant cells</subject><subject>Plant roots</subject><subject>Plants</subject><subject>Protein Transport</subject><subject>Sorting Nexins - genetics</subject><subject>Sorting Nexins - physiology</subject><subject>Statistical significance</subject><issn>1040-4651</issn><issn>1532-298X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNpVkc1v2yAYxtG0ae2yXXfbxHEXd2DwB5dJUeelljJnclypN4QNtHSOSQFX6n9fsnTRdkA88P7e50U8AHzE6AJjlH0N-yEKEleeUvoKnOOMpEnKypvXUSOKEppn-Ay88_4eIYQLzN6Cs5RmLPL4HDxuN21XNyvYVDd1g2HtYaseZuOUhNo6-NPKeRTBTLcw3CnYOaG1GX4fzmKScBtEb0YTnqDVf4ClixfS7r3xsG43TdJWq-v1squ-w65dNttfcVzV4vfgjRajVx9e9gW4_lF1l1fJerOqL5frZKA4DUnZ50xLhplkmjAkeyxJQbAutSSUDXnBskymKGc5Kso8JYKUGVE5K3qaYUY0WYBvR9_93O-UHNQUnBj53pmdcE_cCsP_r0zmjt_aR07jXx3MFuDLi4GzD7Pyge-MH9Q4iknZ2XMc56RZSdABvTiig7PeO6VPYzDih7B4DCsKwo9hxYbP_z7uhP9NJwKfjsC9D9ad6pRgFpOl5BmgJ5cm</recordid><startdate>20140301</startdate><enddate>20140301</enddate><creator>Ivanov, Rumen</creator><creator>Brumbarova, Tzvetina</creator><creator>Blum, Ailisa</creator><creator>Jantke, Anna-Maria</creator><creator>Fink-Straube, Claudia</creator><creator>Bauer, Petra</creator><general>American Society of Plant Biologists</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140301</creationdate><title>SORTING NEXIN1 Is Required for Modulating the Trafficking and Stability of the Arabidopsis IRON-REGULATED TRANSPORTER1</title><author>Ivanov, Rumen ; Brumbarova, Tzvetina ; Blum, Ailisa ; Jantke, Anna-Maria ; Fink-Straube, Claudia ; Bauer, Petra</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-8b69fd919d9f390db1d3731f8fd349c67955d20696078623a3853e697b45193f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis Proteins - physiology</topic><topic>Cation Transport Proteins - metabolism</topic><topic>Endosomes</topic><topic>Gene expression regulation</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Iron</topic><topic>Iron - metabolism</topic><topic>Iron regulatory proteins</topic><topic>Mutation</topic><topic>Phenotypes</topic><topic>Plant cells</topic><topic>Plant roots</topic><topic>Plants</topic><topic>Protein Transport</topic><topic>Sorting Nexins - genetics</topic><topic>Sorting Nexins - physiology</topic><topic>Statistical significance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ivanov, Rumen</creatorcontrib><creatorcontrib>Brumbarova, Tzvetina</creatorcontrib><creatorcontrib>Blum, Ailisa</creatorcontrib><creatorcontrib>Jantke, Anna-Maria</creatorcontrib><creatorcontrib>Fink-Straube, Claudia</creatorcontrib><creatorcontrib>Bauer, Petra</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Plant cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ivanov, Rumen</au><au>Brumbarova, Tzvetina</au><au>Blum, Ailisa</au><au>Jantke, Anna-Maria</au><au>Fink-Straube, Claudia</au><au>Bauer, Petra</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SORTING NEXIN1 Is Required for Modulating the Trafficking and Stability of the Arabidopsis IRON-REGULATED TRANSPORTER1</atitle><jtitle>The Plant cell</jtitle><addtitle>Plant Cell</addtitle><date>2014-03-01</date><risdate>2014</risdate><volume>26</volume><issue>3</issue><spage>1294</spage><epage>1307</epage><pages>1294-1307</pages><issn>1040-4651</issn><eissn>1532-298X</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>www.plantcell.org/cgi/doi/10.1105/tpc.113.116244</notes><notes>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Rumen Ivanov (rumen.ivanov@uni-duesseldorf.de).</notes><abstract>Dicotyledonous plants growing under limited iron availability initiate a response resulting in the solubilization, reduction, and uptake of soil iron. The protein factors responsible for these steps are transmembrane proteins, suggesting that the intracellular trafficking machinery may be involved in iron acquisition. In search for components involved in the regulation of Arabidopsis thaliana iron deficiency responses, we identified the members of the SORTING NEXIN (SNX) protein family. SNX loss-of-function plants display enhanced susceptibility to iron deficiency in comparison to the wild type. The absence of SNX led to reduced iron import efficiency into the root. SNX1 showed partial colocalization with the principal root iron importer IRON-REGULATED TRANSPORTER1 (IRT1). In SNX loss-of-function plants, IRT1 protein levels were decreased compared with the wild type due to enhanced IRT1 degradation. This resulted in diminished amounts of the IRT1 protein at the plasma membrane, snx mutants exhibited enhanced iron deficiency responses compared with the wild type, presumably due to the lower iron uptake through IRT1. Our results reveal a role of SNX1 for the correct trafficking of IRT1 and, thus, for modulating the activity of the iron uptake machinery.</abstract><cop>United States</cop><pub>American Society of Plant Biologists</pub><pmid>24596241</pmid><doi>10.1105/tpc.113.116244</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Plant cell, 2014-03, Vol.26 (3), p.1294-1307 |
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| language | eng |
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| source | JSTOR Archival Journals and Primary Sources Collection; OUP_牛津大学出版社现刊 |
| subjects | Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis Proteins - physiology Cation Transport Proteins - metabolism Endosomes Gene expression regulation Gene Expression Regulation, Plant Genes Iron Iron - metabolism Iron regulatory proteins Mutation Phenotypes Plant cells Plant roots Plants Protein Transport Sorting Nexins - genetics Sorting Nexins - physiology Statistical significance |
| title | SORTING NEXIN1 Is Required for Modulating the Trafficking and Stability of the Arabidopsis IRON-REGULATED TRANSPORTER1 |
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