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The injury response of aged tendons in the absence of biglycan and decorin
Recent studies have demonstrated that the small leucine-rich proteoglycans (SLRPs) biglycan and decorin impact tendon development, aging and healing in mature mice. However, despite the increased risk of tendon injury in the elderly, the role of SLRPs in tendon repair has not been investigated in ag...
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| Published in: | Matrix biology 2014-04, Vol.35, p.232-238 |
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| creator | Dunkman, Andrew A. Buckley, Mark R. Mienaltowski, Michael J. Adams, Sheila M. Thomas, Stephen J. Kumar, Akash Beason, David P. Iozzo, Renato V. Birk, David E. Soslowsky, Louis J. |
| description | Recent studies have demonstrated that the small leucine-rich proteoglycans (SLRPs) biglycan and decorin impact tendon development, aging and healing in mature mice. However, despite the increased risk of tendon injury in the elderly, the role of SLRPs in tendon repair has not been investigated in aged animals. Therefore, our objective was to elucidate the influences of bigylcan and decorin on tendon healing in aged mice to relate our findings to previous work in mature mice. Since the processes of aging and healing are known to interact, our hypothesis was that aging mediates the role of biglycan and decorin on tendon healing. Patellar tendons from wild-type, biglycan-null and decorin-null mice were injured at 270days using an established model. At 3 and 6weeks post-surgery, structural, mechanical and biochemical analyses were performed and compared to uninjured controls. Early stage healing was inferior in biglycan-null and decorin-null mice as compared to wild type. However, tendons of all genotypes failed to exhibit improved mechanical properties between 3 and 6weeks post-injury. In contrast, in a previous investigation of tendon healing in mature (i.e., 120day-old) mice, only biglycan-null mice were deficient in early stage healing while decorin-null mice were deficient in late-stage healing. These results confirm that the impact of SLRPs on tendon healing is mediated by age and could inform future age-specific therapies for enhancing tendon healing.
•We studied how the effect of proteoglycans on tendon healing is impacted by aging.•Early stage healing of aged tendons was inferior without biglycan or decorin.•Healing from 3 to 6weeks post-injury was absent in aged tendons of all genotypes.•The impact of biglycan and decorin on tendon healing is mediated by age. |
| doi_str_mv | 10.1016/j.matbio.2013.10.008 |
| format | article |
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•We studied how the effect of proteoglycans on tendon healing is impacted by aging.•Early stage healing of aged tendons was inferior without biglycan or decorin.•Healing from 3 to 6weeks post-injury was absent in aged tendons of all genotypes.•The impact of biglycan and decorin on tendon healing is mediated by age.</description><identifier>ISSN: 0945-053X</identifier><identifier>EISSN: 1569-1802</identifier><identifier>DOI: 10.1016/j.matbio.2013.10.008</identifier><identifier>PMID: 24157578</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aging ; Aging - pathology ; Aging - physiology ; Animals ; Biglycan ; Biglycan - genetics ; Biglycan - metabolism ; Biomechanical Phenomena ; Decorin ; Decorin - genetics ; Decorin - metabolism ; DNA Primers - genetics ; Female ; Injury ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Microscopy, Electron, Transmission ; Patellar Ligament - injuries ; Proteoglycan ; Reverse Transcriptase Polymerase Chain Reaction ; Tendon ; Tendon Injuries - metabolism ; Wound Healing - genetics ; Wound Healing - physiology</subject><ispartof>Matrix biology, 2014-04, Vol.35, p.232-238</ispartof><rights>2013 International Society of Matrix Biology</rights><rights>2013.</rights><rights>2013 Elsevier B.V. All rights reserved 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c529t-3f2d79f902d7aa01ad9a5be92e4a1fffb7793368e25b33bca2630e6100e19f323</citedby><cites>FETCH-LOGICAL-c529t-3f2d79f902d7aa01ad9a5be92e4a1fffb7793368e25b33bca2630e6100e19f323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,786,790,891,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24157578$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dunkman, Andrew A.</creatorcontrib><creatorcontrib>Buckley, Mark R.</creatorcontrib><creatorcontrib>Mienaltowski, Michael J.</creatorcontrib><creatorcontrib>Adams, Sheila M.</creatorcontrib><creatorcontrib>Thomas, Stephen J.</creatorcontrib><creatorcontrib>Kumar, Akash</creatorcontrib><creatorcontrib>Beason, David P.</creatorcontrib><creatorcontrib>Iozzo, Renato V.</creatorcontrib><creatorcontrib>Birk, David E.</creatorcontrib><creatorcontrib>Soslowsky, Louis J.</creatorcontrib><title>The injury response of aged tendons in the absence of biglycan and decorin</title><title>Matrix biology</title><addtitle>Matrix Biol</addtitle><description>Recent studies have demonstrated that the small leucine-rich proteoglycans (SLRPs) biglycan and decorin impact tendon development, aging and healing in mature mice. However, despite the increased risk of tendon injury in the elderly, the role of SLRPs in tendon repair has not been investigated in aged animals. Therefore, our objective was to elucidate the influences of bigylcan and decorin on tendon healing in aged mice to relate our findings to previous work in mature mice. Since the processes of aging and healing are known to interact, our hypothesis was that aging mediates the role of biglycan and decorin on tendon healing. Patellar tendons from wild-type, biglycan-null and decorin-null mice were injured at 270days using an established model. At 3 and 6weeks post-surgery, structural, mechanical and biochemical analyses were performed and compared to uninjured controls. Early stage healing was inferior in biglycan-null and decorin-null mice as compared to wild type. However, tendons of all genotypes failed to exhibit improved mechanical properties between 3 and 6weeks post-injury. In contrast, in a previous investigation of tendon healing in mature (i.e., 120day-old) mice, only biglycan-null mice were deficient in early stage healing while decorin-null mice were deficient in late-stage healing. These results confirm that the impact of SLRPs on tendon healing is mediated by age and could inform future age-specific therapies for enhancing tendon healing.
•We studied how the effect of proteoglycans on tendon healing is impacted by aging.•Early stage healing of aged tendons was inferior without biglycan or decorin.•Healing from 3 to 6weeks post-injury was absent in aged tendons of all genotypes.•The impact of biglycan and decorin on tendon healing is mediated by age.</description><subject>Aging</subject><subject>Aging - pathology</subject><subject>Aging - physiology</subject><subject>Animals</subject><subject>Biglycan</subject><subject>Biglycan - genetics</subject><subject>Biglycan - metabolism</subject><subject>Biomechanical Phenomena</subject><subject>Decorin</subject><subject>Decorin - genetics</subject><subject>Decorin - metabolism</subject><subject>DNA Primers - genetics</subject><subject>Female</subject><subject>Injury</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Transgenic</subject><subject>Microscopy, Electron, Transmission</subject><subject>Patellar Ligament - injuries</subject><subject>Proteoglycan</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Tendon</subject><subject>Tendon Injuries - metabolism</subject><subject>Wound Healing - genetics</subject><subject>Wound Healing - physiology</subject><issn>0945-053X</issn><issn>1569-1802</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp9UU1rGzEUFKUhcdL-gxL22Mu6T9Jqd3UplNA2CYFcEshNaKUnR2YtudLa4H9fOc5He8npwcy8edIMIV8ozCnQ9ttyvtLT4OOcAeUFmgP0H8iMilbWtAf2kcxANqIGwR9OyGnOSwBomq4_JiesoaITXT8j13ePWPmw3KRdlTCvY8hYRVfpBdpqwmALUPhqKjI9ZAzmiR78YtwZHSodbGXRxOTDJ3Lk9Jjx8_M8I_e_ft5dXNY3t7-vLn7c1EYwOdXcMdtJJ6EMrYFqK7UYUDJsNHXODV0nOW97ZGLgfDCatRywpQBIpeOMn5HvB9_1ZlihNRimpEe1Tn6l005F7dX_TPCPahG3ikvZ0K4tBl-fDVL8s8E8qZXPBsdRB4ybrKjgIAV0vSjS5iA1Keac0L2eoaD2NailOtSg9jXs0VJDWTv_94mvSy-5v_0BS1Bbj0ll4_fhWp_QTMpG__6FvwsWm_4</recordid><startdate>20140401</startdate><enddate>20140401</enddate><creator>Dunkman, Andrew A.</creator><creator>Buckley, Mark R.</creator><creator>Mienaltowski, Michael J.</creator><creator>Adams, Sheila M.</creator><creator>Thomas, Stephen J.</creator><creator>Kumar, Akash</creator><creator>Beason, David P.</creator><creator>Iozzo, Renato V.</creator><creator>Birk, David E.</creator><creator>Soslowsky, Louis J.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140401</creationdate><title>The injury response of aged tendons in the absence of biglycan and decorin</title><author>Dunkman, Andrew A. ; Buckley, Mark R. ; Mienaltowski, Michael J. ; Adams, Sheila M. ; Thomas, Stephen J. ; Kumar, Akash ; Beason, David P. ; Iozzo, Renato V. ; Birk, David E. ; Soslowsky, Louis J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c529t-3f2d79f902d7aa01ad9a5be92e4a1fffb7793368e25b33bca2630e6100e19f323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Aging</topic><topic>Aging - pathology</topic><topic>Aging - physiology</topic><topic>Animals</topic><topic>Biglycan</topic><topic>Biglycan - genetics</topic><topic>Biglycan - metabolism</topic><topic>Biomechanical Phenomena</topic><topic>Decorin</topic><topic>Decorin - genetics</topic><topic>Decorin - metabolism</topic><topic>DNA Primers - genetics</topic><topic>Female</topic><topic>Injury</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Transgenic</topic><topic>Microscopy, Electron, Transmission</topic><topic>Patellar Ligament - injuries</topic><topic>Proteoglycan</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Tendon</topic><topic>Tendon Injuries - metabolism</topic><topic>Wound Healing - genetics</topic><topic>Wound Healing - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dunkman, Andrew A.</creatorcontrib><creatorcontrib>Buckley, Mark R.</creatorcontrib><creatorcontrib>Mienaltowski, Michael J.</creatorcontrib><creatorcontrib>Adams, Sheila M.</creatorcontrib><creatorcontrib>Thomas, Stephen J.</creatorcontrib><creatorcontrib>Kumar, Akash</creatorcontrib><creatorcontrib>Beason, David P.</creatorcontrib><creatorcontrib>Iozzo, Renato V.</creatorcontrib><creatorcontrib>Birk, David E.</creatorcontrib><creatorcontrib>Soslowsky, Louis J.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Matrix biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dunkman, Andrew A.</au><au>Buckley, Mark R.</au><au>Mienaltowski, Michael J.</au><au>Adams, Sheila M.</au><au>Thomas, Stephen J.</au><au>Kumar, Akash</au><au>Beason, David P.</au><au>Iozzo, Renato V.</au><au>Birk, David E.</au><au>Soslowsky, Louis J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The injury response of aged tendons in the absence of biglycan and decorin</atitle><jtitle>Matrix biology</jtitle><addtitle>Matrix Biol</addtitle><date>2014-04-01</date><risdate>2014</risdate><volume>35</volume><spage>232</spage><epage>238</epage><pages>232-238</pages><issn>0945-053X</issn><eissn>1569-1802</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>Co-first authors A.A. Dunkman and M.R. Buckley contributed equally to this work.</notes><abstract>Recent studies have demonstrated that the small leucine-rich proteoglycans (SLRPs) biglycan and decorin impact tendon development, aging and healing in mature mice. However, despite the increased risk of tendon injury in the elderly, the role of SLRPs in tendon repair has not been investigated in aged animals. Therefore, our objective was to elucidate the influences of bigylcan and decorin on tendon healing in aged mice to relate our findings to previous work in mature mice. Since the processes of aging and healing are known to interact, our hypothesis was that aging mediates the role of biglycan and decorin on tendon healing. Patellar tendons from wild-type, biglycan-null and decorin-null mice were injured at 270days using an established model. At 3 and 6weeks post-surgery, structural, mechanical and biochemical analyses were performed and compared to uninjured controls. Early stage healing was inferior in biglycan-null and decorin-null mice as compared to wild type. However, tendons of all genotypes failed to exhibit improved mechanical properties between 3 and 6weeks post-injury. In contrast, in a previous investigation of tendon healing in mature (i.e., 120day-old) mice, only biglycan-null mice were deficient in early stage healing while decorin-null mice were deficient in late-stage healing. These results confirm that the impact of SLRPs on tendon healing is mediated by age and could inform future age-specific therapies for enhancing tendon healing.
•We studied how the effect of proteoglycans on tendon healing is impacted by aging.•Early stage healing of aged tendons was inferior without biglycan or decorin.•Healing from 3 to 6weeks post-injury was absent in aged tendons of all genotypes.•The impact of biglycan and decorin on tendon healing is mediated by age.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>24157578</pmid><doi>10.1016/j.matbio.2013.10.008</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Matrix biology, 2014-04, Vol.35, p.232-238 |
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| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Aging Aging - pathology Aging - physiology Animals Biglycan Biglycan - genetics Biglycan - metabolism Biomechanical Phenomena Decorin Decorin - genetics Decorin - metabolism DNA Primers - genetics Female Injury Mice Mice, Inbred C57BL Mice, Transgenic Microscopy, Electron, Transmission Patellar Ligament - injuries Proteoglycan Reverse Transcriptase Polymerase Chain Reaction Tendon Tendon Injuries - metabolism Wound Healing - genetics Wound Healing - physiology |
| title | The injury response of aged tendons in the absence of biglycan and decorin |
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