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Host serum miR-223 is a potential new biomarker for Schistosoma japonicum infection and the response to chemotherapy
BACKGROUND: Numerous studies have shown that aberrant microRNA (miRNA) expression is associated with the pathogenesis and progression of various human diseases. Hence, serum miRNAs are considered to be potential biomarkers for the diagnosis of human diseases. This study examined whether several miRN...
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| Published in: | Parasites & vectors 2013-09, Vol.6 (1), p.272-272, Article 272 |
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| creator | He, Xing Sai, Xue Chen, Chao Zhang, Yuanbin Xu, Xindong Zhang, Dongmei Pan, Weiqing |
| description | BACKGROUND: Numerous studies have shown that aberrant microRNA (miRNA) expression is associated with the pathogenesis and progression of various human diseases. Hence, serum miRNAs are considered to be potential biomarkers for the diagnosis of human diseases. This study examined whether several miRNAs known to be commonly deregulated in liver diseases are deregulated in the serum of hosts with hepatic schistosomiasis, and thus whether they could serve as potential markers for detection of schistosome infection and evaluation of the effectiveness of chemotherapy. METHODS: We analyzed the serum levels of six selected candidate miRNA molecules (miR-146b, miR-122, miR-223, miR-199a-5p, miR-199a-3p, miR-34a) from mice, rabbits, buffalos and humans infected with Schistosoma japonicum using qPCR. We evaluated liver pathology by determining the hydroxyproline content in liver tissues. Primary resident liver cells were isolated to quantify the expression level of deregulated miRNAs. Bioinformatics analyses were also conducted to assess the potential function of miR-223. RESULTS: Using a mouse model of Schistosoma japonicum infection, we found that the expression level of serum miR-223 was significantly elevated after infection, but returned to near normal levels after the treatment with praziquantel (PZQ). Importantly, the level of serum miR-223 reflected the extent of liver pathology post-infection. We validated the elevated level of the circulating miR-223 in serum samples of other host species including rabbits, buffalos and humans. In addition, our results showed that miR-223 was primarily located in the Kupffer cells, but its expression levels were significantly up-regulated in hepatocytes, hepatic stellate cells and Kupffer cells after infection. Bioinformatics analyses revealed a potential functional role of miR-223 in transcription regulator activity, transcription factor activity and DNA binding. CONCLUSIONS: This study suggested that the circulating miR-223 could serve as a potential new biomarker for the detection of schistosome infection and the assessment of the response to chemotherapy. |
| doi_str_mv | 10.1186/1756-3305-6-272 |
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Hence, serum miRNAs are considered to be potential biomarkers for the diagnosis of human diseases. This study examined whether several miRNAs known to be commonly deregulated in liver diseases are deregulated in the serum of hosts with hepatic schistosomiasis, and thus whether they could serve as potential markers for detection of schistosome infection and evaluation of the effectiveness of chemotherapy. METHODS: We analyzed the serum levels of six selected candidate miRNA molecules (miR-146b, miR-122, miR-223, miR-199a-5p, miR-199a-3p, miR-34a) from mice, rabbits, buffalos and humans infected with Schistosoma japonicum using qPCR. We evaluated liver pathology by determining the hydroxyproline content in liver tissues. Primary resident liver cells were isolated to quantify the expression level of deregulated miRNAs. Bioinformatics analyses were also conducted to assess the potential function of miR-223. RESULTS: Using a mouse model of Schistosoma japonicum infection, we found that the expression level of serum miR-223 was significantly elevated after infection, but returned to near normal levels after the treatment with praziquantel (PZQ). Importantly, the level of serum miR-223 reflected the extent of liver pathology post-infection. We validated the elevated level of the circulating miR-223 in serum samples of other host species including rabbits, buffalos and humans. In addition, our results showed that miR-223 was primarily located in the Kupffer cells, but its expression levels were significantly up-regulated in hepatocytes, hepatic stellate cells and Kupffer cells after infection. Bioinformatics analyses revealed a potential functional role of miR-223 in transcription regulator activity, transcription factor activity and DNA binding. CONCLUSIONS: This study suggested that the circulating miR-223 could serve as a potential new biomarker for the detection of schistosome infection and the assessment of the response to chemotherapy.</description><identifier>ISSN: 1756-3305</identifier><identifier>EISSN: 1756-3305</identifier><identifier>DOI: 10.1186/1756-3305-6-272</identifier><identifier>PMID: 24330517</identifier><language>eng</language><publisher>England: Springer-Verlag</publisher><subject>Analysis ; Animals ; bioinformatics ; Biological markers ; Biomarkers ; Biomarkers - blood ; blood serum ; Buffaloes ; Cancer ; Care and treatment ; Chemotherapy ; Diagnosis ; DNA ; Eggs ; Gene expression ; Gene Expression Regulation ; Genetic aspects ; Genetic transcription ; Health aspects ; hepatocytes ; hosts ; human diseases ; Humans ; hydroxyproline ; Hydroxyproline - metabolism ; Infection ; Kupffer cells ; Liver ; Liver diseases ; Male ; Medical genetics ; Medical research ; Medicine, Experimental ; Mice ; Mice, Inbred BALB C ; MicroRNA ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Military medicine ; Mortality ; Parasites ; Parasitic diseases ; pathogenesis ; praziquantel ; quantitative polymerase chain reaction ; Rabbits ; Schistosoma japonicum ; Schistosoma japonicum - physiology ; Schistosomiasis ; Schistosomiasis japonica - blood ; Schistosomiasis japonica - drug therapy ; Schistosomiasis japonica - parasitology ; Schistosomicides - therapeutic use ; transcription factors ; Tropical diseases</subject><ispartof>Parasites & vectors, 2013-09, Vol.6 (1), p.272-272, Article 272</ispartof><rights>COPYRIGHT 2013 BioMed Central Ltd.</rights><rights>2013 He et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2013 He et al.; licensee BioMed Central Ltd. 2013 He et al.; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b704t-4e209b27b80fd1035e037802d39553816f650945303f07777d1297395ce83efa3</citedby><cites>FETCH-LOGICAL-b704t-4e209b27b80fd1035e037802d39553816f650945303f07777d1297395ce83efa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3856452/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1437021239?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,315,733,786,790,891,25783,27957,27958,37047,37048,44625,53827,53829</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24330517$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Xing</creatorcontrib><creatorcontrib>Sai, Xue</creatorcontrib><creatorcontrib>Chen, Chao</creatorcontrib><creatorcontrib>Zhang, Yuanbin</creatorcontrib><creatorcontrib>Xu, Xindong</creatorcontrib><creatorcontrib>Zhang, Dongmei</creatorcontrib><creatorcontrib>Pan, Weiqing</creatorcontrib><title>Host serum miR-223 is a potential new biomarker for Schistosoma japonicum infection and the response to chemotherapy</title><title>Parasites & vectors</title><addtitle>Parasit Vectors</addtitle><description>BACKGROUND: Numerous studies have shown that aberrant microRNA (miRNA) expression is associated with the pathogenesis and progression of various human diseases. Hence, serum miRNAs are considered to be potential biomarkers for the diagnosis of human diseases. This study examined whether several miRNAs known to be commonly deregulated in liver diseases are deregulated in the serum of hosts with hepatic schistosomiasis, and thus whether they could serve as potential markers for detection of schistosome infection and evaluation of the effectiveness of chemotherapy. METHODS: We analyzed the serum levels of six selected candidate miRNA molecules (miR-146b, miR-122, miR-223, miR-199a-5p, miR-199a-3p, miR-34a) from mice, rabbits, buffalos and humans infected with Schistosoma japonicum using qPCR. We evaluated liver pathology by determining the hydroxyproline content in liver tissues. Primary resident liver cells were isolated to quantify the expression level of deregulated miRNAs. Bioinformatics analyses were also conducted to assess the potential function of miR-223. RESULTS: Using a mouse model of Schistosoma japonicum infection, we found that the expression level of serum miR-223 was significantly elevated after infection, but returned to near normal levels after the treatment with praziquantel (PZQ). Importantly, the level of serum miR-223 reflected the extent of liver pathology post-infection. We validated the elevated level of the circulating miR-223 in serum samples of other host species including rabbits, buffalos and humans. In addition, our results showed that miR-223 was primarily located in the Kupffer cells, but its expression levels were significantly up-regulated in hepatocytes, hepatic stellate cells and Kupffer cells after infection. Bioinformatics analyses revealed a potential functional role of miR-223 in transcription regulator activity, transcription factor activity and DNA binding. CONCLUSIONS: This study suggested that the circulating miR-223 could serve as a potential new biomarker for the detection of schistosome infection and the assessment of the response to chemotherapy.</description><subject>Analysis</subject><subject>Animals</subject><subject>bioinformatics</subject><subject>Biological markers</subject><subject>Biomarkers</subject><subject>Biomarkers - blood</subject><subject>blood serum</subject><subject>Buffaloes</subject><subject>Cancer</subject><subject>Care and treatment</subject><subject>Chemotherapy</subject><subject>Diagnosis</subject><subject>DNA</subject><subject>Eggs</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Genetic aspects</subject><subject>Genetic transcription</subject><subject>Health aspects</subject><subject>hepatocytes</subject><subject>hosts</subject><subject>human diseases</subject><subject>Humans</subject><subject>hydroxyproline</subject><subject>Hydroxyproline - metabolism</subject><subject>Infection</subject><subject>Kupffer cells</subject><subject>Liver</subject><subject>Liver diseases</subject><subject>Male</subject><subject>Medical genetics</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>MicroRNA</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Military medicine</subject><subject>Mortality</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>pathogenesis</subject><subject>praziquantel</subject><subject>quantitative polymerase chain reaction</subject><subject>Rabbits</subject><subject>Schistosoma japonicum</subject><subject>Schistosoma japonicum - physiology</subject><subject>Schistosomiasis</subject><subject>Schistosomiasis japonica - blood</subject><subject>Schistosomiasis japonica - drug therapy</subject><subject>Schistosomiasis japonica - parasitology</subject><subject>Schistosomicides - therapeutic use</subject><subject>transcription factors</subject><subject>Tropical diseases</subject><issn>1756-3305</issn><issn>1756-3305</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNqFks1v1DAQxSMEoqVw5gaWuMAhrT9iO7kglQpopUpIXXq2vMlk10sSL7YD9L9noi1Lg1YiOSR685snzxtn2UtGTxkr1RnTUuVCUJmrnGv-KDveK48f_B9lz2LcUKpoJdXT7IgXk8r0cZYufUwkQhh70rubnHNBXCSWbH2CITnbkQF-kqXzvQ3fIJDWB7Ko1y4mH1EjG7v1g6ux3Q0t1Mn5gdihIWkNJEDEYgSSPKnX0HsUg93ePc-etLaL8OL-e5Ldfvr49eIyv_7y-eri_DpfalqkvABOqyXXy5K2DaNCAhW6pLwRlZSiZKpVklaFFFS0VOPTMF5pLNZQCmitOMne73y347KHpsaBgu3MNjgc5s5468y8Mri1WfkfRpRSFZKjwYedwTT_YYN5pfa9mVI3U75GGdwJmry9P0Xw30eIyfQu1tB1dgA_RsMkx7mUZur_aKEqziiTE_rmH3TjxzBgnEgJTTnjovpLrWwHBjfk8Zj1ZGrOpSh0yXQlkDo9QOHbQO9qP0DrUJ81vJs1IJPgV1rZMUZztbiZs2c7tg4-xgDtPj5GzXSJDwT26uHa9vyfW4vA6x3QWm_sKrhobhccY6GUlhUrpfgN3FHy1Q</recordid><startdate>20130920</startdate><enddate>20130920</enddate><creator>He, Xing</creator><creator>Sai, Xue</creator><creator>Chen, Chao</creator><creator>Zhang, Yuanbin</creator><creator>Xu, Xindong</creator><creator>Zhang, Dongmei</creator><creator>Pan, Weiqing</creator><general>Springer-Verlag</general><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H95</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>H97</scope><scope>5PM</scope></search><sort><creationdate>20130920</creationdate><title>Host serum miR-223 is a potential new biomarker for Schistosoma japonicum infection and the response to chemotherapy</title><author>He, Xing ; Sai, Xue ; Chen, Chao ; Zhang, Yuanbin ; Xu, Xindong ; Zhang, Dongmei ; Pan, Weiqing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b704t-4e209b27b80fd1035e037802d39553816f650945303f07777d1297395ce83efa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>bioinformatics</topic><topic>Biological markers</topic><topic>Biomarkers</topic><topic>Biomarkers - blood</topic><topic>blood serum</topic><topic>Buffaloes</topic><topic>Cancer</topic><topic>Care and treatment</topic><topic>Chemotherapy</topic><topic>Diagnosis</topic><topic>DNA</topic><topic>Eggs</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Genetic aspects</topic><topic>Genetic transcription</topic><topic>Health aspects</topic><topic>hepatocytes</topic><topic>hosts</topic><topic>human diseases</topic><topic>Humans</topic><topic>hydroxyproline</topic><topic>Hydroxyproline - metabolism</topic><topic>Infection</topic><topic>Kupffer cells</topic><topic>Liver</topic><topic>Liver diseases</topic><topic>Male</topic><topic>Medical genetics</topic><topic>Medical research</topic><topic>Medicine, Experimental</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>MicroRNA</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Military medicine</topic><topic>Mortality</topic><topic>Parasites</topic><topic>Parasitic diseases</topic><topic>pathogenesis</topic><topic>praziquantel</topic><topic>quantitative polymerase chain reaction</topic><topic>Rabbits</topic><topic>Schistosoma japonicum</topic><topic>Schistosoma japonicum - physiology</topic><topic>Schistosomiasis</topic><topic>Schistosomiasis japonica - blood</topic><topic>Schistosomiasis japonica - drug therapy</topic><topic>Schistosomiasis japonica - parasitology</topic><topic>Schistosomicides - therapeutic use</topic><topic>transcription factors</topic><topic>Tropical diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Xing</creatorcontrib><creatorcontrib>Sai, Xue</creatorcontrib><creatorcontrib>Chen, Chao</creatorcontrib><creatorcontrib>Zhang, Yuanbin</creatorcontrib><creatorcontrib>Xu, Xindong</creatorcontrib><creatorcontrib>Zhang, Dongmei</creatorcontrib><creatorcontrib>Pan, Weiqing</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest - 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Hence, serum miRNAs are considered to be potential biomarkers for the diagnosis of human diseases. This study examined whether several miRNAs known to be commonly deregulated in liver diseases are deregulated in the serum of hosts with hepatic schistosomiasis, and thus whether they could serve as potential markers for detection of schistosome infection and evaluation of the effectiveness of chemotherapy. METHODS: We analyzed the serum levels of six selected candidate miRNA molecules (miR-146b, miR-122, miR-223, miR-199a-5p, miR-199a-3p, miR-34a) from mice, rabbits, buffalos and humans infected with Schistosoma japonicum using qPCR. We evaluated liver pathology by determining the hydroxyproline content in liver tissues. Primary resident liver cells were isolated to quantify the expression level of deregulated miRNAs. Bioinformatics analyses were also conducted to assess the potential function of miR-223. RESULTS: Using a mouse model of Schistosoma japonicum infection, we found that the expression level of serum miR-223 was significantly elevated after infection, but returned to near normal levels after the treatment with praziquantel (PZQ). Importantly, the level of serum miR-223 reflected the extent of liver pathology post-infection. We validated the elevated level of the circulating miR-223 in serum samples of other host species including rabbits, buffalos and humans. In addition, our results showed that miR-223 was primarily located in the Kupffer cells, but its expression levels were significantly up-regulated in hepatocytes, hepatic stellate cells and Kupffer cells after infection. Bioinformatics analyses revealed a potential functional role of miR-223 in transcription regulator activity, transcription factor activity and DNA binding. CONCLUSIONS: This study suggested that the circulating miR-223 could serve as a potential new biomarker for the detection of schistosome infection and the assessment of the response to chemotherapy.</abstract><cop>England</cop><pub>Springer-Verlag</pub><pmid>24330517</pmid><doi>10.1186/1756-3305-6-272</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Parasites & vectors, 2013-09, Vol.6 (1), p.272-272, Article 272 |
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| source | ProQuest - Publicly Available Content Database; PubMed Central |
| subjects | Analysis Animals bioinformatics Biological markers Biomarkers Biomarkers - blood blood serum Buffaloes Cancer Care and treatment Chemotherapy Diagnosis DNA Eggs Gene expression Gene Expression Regulation Genetic aspects Genetic transcription Health aspects hepatocytes hosts human diseases Humans hydroxyproline Hydroxyproline - metabolism Infection Kupffer cells Liver Liver diseases Male Medical genetics Medical research Medicine, Experimental Mice Mice, Inbred BALB C MicroRNA MicroRNAs - genetics MicroRNAs - metabolism Military medicine Mortality Parasites Parasitic diseases pathogenesis praziquantel quantitative polymerase chain reaction Rabbits Schistosoma japonicum Schistosoma japonicum - physiology Schistosomiasis Schistosomiasis japonica - blood Schistosomiasis japonica - drug therapy Schistosomiasis japonica - parasitology Schistosomicides - therapeutic use transcription factors Tropical diseases |
| title | Host serum miR-223 is a potential new biomarker for Schistosoma japonicum infection and the response to chemotherapy |
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