Separation of mandelic acid and its derivatives with new immobilized cellulose chiral stationary phase

A new liquid chromatographic method has been developed for the chiral separation of the enantiomers of mandelic acid and their derivatives 2-chioromandelic acid, 4-hydroxymandelic acid, 4-methoxymandelic acid, and 3,4,5-trismethoxymandelic acid. The enantiomers were separated by a CHIRALPAK IC （250...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Zhejiang University. B. Science 2013-07, Vol.14 (7), p.615-620
Main Authors: Zhou, Jie, Liu, Qian, Fu, Guang-jun, Zhang, Zhen-zhong
Format: Article
Language:English
Subjects:
Alcohols - chemistry
Biomedical and Life Sciences
Biomedicine
Biotechnology
Cellulose - chemistry
Chemistry Techniques, Analytical
Chromatography, High Pressure Liquid - methods
Mandelic Acids - analysis
Mandelic Acids - isolation & purification
Stereoisomerism
Temperature
Trifluoroacetic Acid - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A new liquid chromatographic method has been developed for the chiral separation of the enantiomers of mandelic acid and their derivatives 2-chioromandelic acid, 4-hydroxymandelic acid, 4-methoxymandelic acid, and 3,4,5-trismethoxymandelic acid. The enantiomers were separated by a CHIRALPAK IC （250 mmx4.6 mm, 5 pm）. Mandelic acid, 4-methoxymandelic acid, and 3,4,5-trismethoxymandelic acid were baseline resolved （resolution factor （Rs）=2.21, Rs=2.14, and Rs=3.70, respectively）. In contrast, the enantioselectivities between CHIRALPAK IC and 2-chloromandelic acid and 4-hydroxymandelic acid investigated were low. By comparing the chromatographs of mandelic acid enantiomers and mandelic acid spiked with （R）-mandelic acid, it was determined that the first effluent was （R）-mandelic acid.
ISSN:1673-1581
1862-1783
DOI:10.1631/jzus.B1200361