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Programmed cell death induced by high levels of cytokinin in Arabidopsis cultured cells is mediated by the cytokinin receptor CRE1/AHK4
High levels of cytokinins (CKs) induce programmed cell death (PCD) both in animals and plant cells. High levels of the CK benzylaminopurine (BA) induce PCD in cultured cells of Arabidopsis thaliana by accelerating a senescence process characterized by DNA laddering and expression of a specific senes...
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| Published in: | Journal of experimental botany 2012-04, Vol.63 (7), p.2825-2832 |
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| cites | cdi_FETCH-LOGICAL-c441t-99863d1a05e83e7365a4b9baf1d8b031d593943f20a2384a53a4751b68a243ce3 |
| container_end_page | 2832 |
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| container_title | Journal of experimental botany |
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| creator | VESCOVI, Marco RIEFLER, Michael GESSUTI, Micael NOVAK, Ondřej SCHMÜLLING, Thomas LO SCHIAVO, Fiorella |
| description | High levels of cytokinins (CKs) induce programmed cell death (PCD) both in animals and plant cells. High levels of the CK benzylaminopurine (BA) induce PCD in cultured cells of Arabidopsis thaliana by accelerating a senescence process characterized by DNA laddering and expression of a specific senescence marker. In this report, the question has been addressed whether members of the small family of Arabidopsis CK receptors (AHK2, AHK3, CRE1/AHK4) are required for BA-induced PCD. In this respect, suspension cell cultures were produced from selected receptor mutants. Cell growth and proliferation of all receptor mutant and wild-type cell cultures were similar, showing that the CK receptors are not required for these processes in cultured cells. The analysis of CK metabolites instead revealed differences between wild-type and receptor mutant lines, and indicated that all three receptors are redundantly involved in the regulation of the steady-state levels of isopentenyladenine- and trans-zeatin-type CKs. By contrast, the levels of cis-zeatin-type CKs were controlled mainly by AHK2 and AHK3. To study the role of CK receptors in the BA-induced PCD pathway, cultured cells were analysed for their behaviour in the presence of high levels of BA. The results show that CRE1/AHK4, the strongest expressed CK receptor gene of this family in cultured cells, is required for PCD, thus linking this process to the known CK signalling pathway. |
| doi_str_mv | 10.1093/jxb/ers008 |
| format | article |
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High levels of the CK benzylaminopurine (BA) induce PCD in cultured cells of Arabidopsis thaliana by accelerating a senescence process characterized by DNA laddering and expression of a specific senescence marker. In this report, the question has been addressed whether members of the small family of Arabidopsis CK receptors (AHK2, AHK3, CRE1/AHK4) are required for BA-induced PCD. In this respect, suspension cell cultures were produced from selected receptor mutants. Cell growth and proliferation of all receptor mutant and wild-type cell cultures were similar, showing that the CK receptors are not required for these processes in cultured cells. The analysis of CK metabolites instead revealed differences between wild-type and receptor mutant lines, and indicated that all three receptors are redundantly involved in the regulation of the steady-state levels of isopentenyladenine- and trans-zeatin-type CKs. By contrast, the levels of cis-zeatin-type CKs were controlled mainly by AHK2 and AHK3. To study the role of CK receptors in the BA-induced PCD pathway, cultured cells were analysed for their behaviour in the presence of high levels of BA. The results show that CRE1/AHK4, the strongest expressed CK receptor gene of this family in cultured cells, is required for PCD, thus linking this process to the known CK signalling pathway.</description><identifier>ISSN: 0022-0957</identifier><identifier>EISSN: 1460-2431</identifier><identifier>DOI: 10.1093/jxb/ers008</identifier><identifier>PMID: 22312114</identifier><identifier>CODEN: JEBOA6</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Apoptosis ; Arabidopsis - cytology ; Arabidopsis - enzymology ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Biological and medical sciences ; Cells, Cultured ; Cytokinins - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Plant ; Plant physiology and development ; Protein Kinases - genetics ; Protein Kinases - metabolism ; Receptors, Cell Surface - genetics ; Receptors, Cell Surface - metabolism ; Research Papers ; Signal Transduction</subject><ispartof>Journal of experimental botany, 2012-04, Vol.63 (7), p.2825-2832</ispartof><rights>2015 INIST-CNRS</rights><rights>2012 The Author(s). 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-99863d1a05e83e7365a4b9baf1d8b031d593943f20a2384a53a4751b68a243ce3</citedby><cites>FETCH-LOGICAL-c441t-99863d1a05e83e7365a4b9baf1d8b031d593943f20a2384a53a4751b68a243ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,786,790,891,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25924909$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22312114$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>VESCOVI, Marco</creatorcontrib><creatorcontrib>RIEFLER, Michael</creatorcontrib><creatorcontrib>GESSUTI, Micael</creatorcontrib><creatorcontrib>NOVAK, Ondřej</creatorcontrib><creatorcontrib>SCHMÜLLING, Thomas</creatorcontrib><creatorcontrib>LO SCHIAVO, Fiorella</creatorcontrib><title>Programmed cell death induced by high levels of cytokinin in Arabidopsis cultured cells is mediated by the cytokinin receptor CRE1/AHK4</title><title>Journal of experimental botany</title><addtitle>J Exp Bot</addtitle><description>High levels of cytokinins (CKs) induce programmed cell death (PCD) both in animals and plant cells. High levels of the CK benzylaminopurine (BA) induce PCD in cultured cells of Arabidopsis thaliana by accelerating a senescence process characterized by DNA laddering and expression of a specific senescence marker. In this report, the question has been addressed whether members of the small family of Arabidopsis CK receptors (AHK2, AHK3, CRE1/AHK4) are required for BA-induced PCD. In this respect, suspension cell cultures were produced from selected receptor mutants. Cell growth and proliferation of all receptor mutant and wild-type cell cultures were similar, showing that the CK receptors are not required for these processes in cultured cells. The analysis of CK metabolites instead revealed differences between wild-type and receptor mutant lines, and indicated that all three receptors are redundantly involved in the regulation of the steady-state levels of isopentenyladenine- and trans-zeatin-type CKs. By contrast, the levels of cis-zeatin-type CKs were controlled mainly by AHK2 and AHK3. To study the role of CK receptors in the BA-induced PCD pathway, cultured cells were analysed for their behaviour in the presence of high levels of BA. The results show that CRE1/AHK4, the strongest expressed CK receptor gene of this family in cultured cells, is required for PCD, thus linking this process to the known CK signalling pathway.</description><subject>Apoptosis</subject><subject>Arabidopsis - cytology</subject><subject>Arabidopsis - enzymology</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Cytokinins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Plant</subject><subject>Plant physiology and development</subject><subject>Protein Kinases - genetics</subject><subject>Protein Kinases - metabolism</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Research Papers</subject><subject>Signal Transduction</subject><issn>0022-0957</issn><issn>1460-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqNkc-KFDEQh4Mo7rh68QEkF0GEdir_ejoXYRhWV1xQRM-hOp2eztrTGZP04jyBr22WGdf15qmg6stHVX6EPGfwhoEWy-uf7dLFBNA8IAsma6i4FOwhWQBwXoFWqzPyJKVrAFCg1GNyxrlgnDG5IL8-x7CNuNu5jlo3jrRzmAfqp262pdUe6OC3Ax3djRsTDT21hxy--8lPhaHriK3vwj75RO085jmeNImWTnF6zEdLHty9p9FZt88h0s2XC7ZcX36UT8mjHsfknp3qOfn27uLr5rK6-vT-w2Z9VVkpWa60bmrRMQTlGuFWolYoW91iz7qmBcE6pYWWoueAXDQSlUC5UqytGyx_Yp04J2-P3v3clv2sm3LE0eyj32E8mIDe_DuZ_GC24cYIIWsudBG8Ogli-DG7lM3Op9uTcXJhToYBh0Yy1vwPWjDJV40o6OsjamNIKbr-biMG5jZkU0I2x5AL_OL-DXfon1QL8PIEYLI49hEn69NfTmkudZH-BuLRsVU</recordid><startdate>20120401</startdate><enddate>20120401</enddate><creator>VESCOVI, Marco</creator><creator>RIEFLER, Michael</creator><creator>GESSUTI, Micael</creator><creator>NOVAK, Ondřej</creator><creator>SCHMÜLLING, Thomas</creator><creator>LO SCHIAVO, Fiorella</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7UA</scope><scope>C1K</scope><scope>5PM</scope></search><sort><creationdate>20120401</creationdate><title>Programmed cell death induced by high levels of cytokinin in Arabidopsis cultured cells is mediated by the cytokinin receptor CRE1/AHK4</title><author>VESCOVI, Marco ; RIEFLER, Michael ; GESSUTI, Micael ; NOVAK, Ondřej ; SCHMÜLLING, Thomas ; LO SCHIAVO, Fiorella</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-99863d1a05e83e7365a4b9baf1d8b031d593943f20a2384a53a4751b68a243ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Apoptosis</topic><topic>Arabidopsis - cytology</topic><topic>Arabidopsis - enzymology</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Cytokinins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Plant</topic><topic>Plant physiology and development</topic><topic>Protein Kinases - genetics</topic><topic>Protein Kinases - metabolism</topic><topic>Receptors, Cell Surface - genetics</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Research Papers</topic><topic>Signal Transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>VESCOVI, Marco</creatorcontrib><creatorcontrib>RIEFLER, Michael</creatorcontrib><creatorcontrib>GESSUTI, Micael</creatorcontrib><creatorcontrib>NOVAK, Ondřej</creatorcontrib><creatorcontrib>SCHMÜLLING, Thomas</creatorcontrib><creatorcontrib>LO SCHIAVO, Fiorella</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of experimental botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>VESCOVI, Marco</au><au>RIEFLER, Michael</au><au>GESSUTI, Micael</au><au>NOVAK, Ondřej</au><au>SCHMÜLLING, Thomas</au><au>LO SCHIAVO, Fiorella</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Programmed cell death induced by high levels of cytokinin in Arabidopsis cultured cells is mediated by the cytokinin receptor CRE1/AHK4</atitle><jtitle>Journal of experimental botany</jtitle><addtitle>J Exp Bot</addtitle><date>2012-04-01</date><risdate>2012</risdate><volume>63</volume><issue>7</issue><spage>2825</spage><epage>2832</epage><pages>2825-2832</pages><issn>0022-0957</issn><eissn>1460-2431</eissn><coden>JEBOA6</coden><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>High levels of cytokinins (CKs) induce programmed cell death (PCD) both in animals and plant cells. High levels of the CK benzylaminopurine (BA) induce PCD in cultured cells of Arabidopsis thaliana by accelerating a senescence process characterized by DNA laddering and expression of a specific senescence marker. In this report, the question has been addressed whether members of the small family of Arabidopsis CK receptors (AHK2, AHK3, CRE1/AHK4) are required for BA-induced PCD. In this respect, suspension cell cultures were produced from selected receptor mutants. Cell growth and proliferation of all receptor mutant and wild-type cell cultures were similar, showing that the CK receptors are not required for these processes in cultured cells. The analysis of CK metabolites instead revealed differences between wild-type and receptor mutant lines, and indicated that all three receptors are redundantly involved in the regulation of the steady-state levels of isopentenyladenine- and trans-zeatin-type CKs. By contrast, the levels of cis-zeatin-type CKs were controlled mainly by AHK2 and AHK3. To study the role of CK receptors in the BA-induced PCD pathway, cultured cells were analysed for their behaviour in the presence of high levels of BA. The results show that CRE1/AHK4, the strongest expressed CK receptor gene of this family in cultured cells, is required for PCD, thus linking this process to the known CK signalling pathway.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>22312114</pmid><doi>10.1093/jxb/ers008</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | JSTOR Archival Journals and Primary Sources Collection; OUP_牛津大学出版社现刊 |
| subjects | Apoptosis Arabidopsis - cytology Arabidopsis - enzymology Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Biological and medical sciences Cells, Cultured Cytokinins - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant Plant physiology and development Protein Kinases - genetics Protein Kinases - metabolism Receptors, Cell Surface - genetics Receptors, Cell Surface - metabolism Research Papers Signal Transduction |
| title | Programmed cell death induced by high levels of cytokinin in Arabidopsis cultured cells is mediated by the cytokinin receptor CRE1/AHK4 |
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