Angiotensin II induced catabolic effect and muscle atrophy are redox dependent

► Angiotensin II increases NADPH oxidase-derived ROS in skeletal muscle. ► Deletion of p47 phox prevents Ang II-induced skeletal muscle weight loss. ► Ang II-induced proteasome activity in skeletal muscle is prevented in p47 phox−/− mice. Angiotensin II (Ang II) causes skeletal muscle wasting via an...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2011-06, Vol.409 (2), p.217-221
Main Authors: Semprun-Prieto, Laura C., Sukhanov, Sergiy, Yoshida, Tadashi, Rezk, Bashir M., Gonzalez-Villalobos, Romer A., Vaughn, Charlotte, Michael Tabony, A., Delafontaine, Patrice
Format: Article
Language:English
Subjects:
Angiotensin II
Angiotensin II - adverse effects
Animals
Atrophy
Male
Mice
Mice, Inbred C57BL
Mice, Mutant Strains
Muscle, Skeletal - drug effects
Muscle, Skeletal - metabolism
Muscular Atrophy - chemically induced
Muscular Atrophy - metabolism
NADPH oxidase
NADPH Oxidases - genetics
Oxidation-Reduction
Oxidative stress
Proteasome Endopeptidase Complex - metabolism
Reactive Oxygen Species - metabolism
Skeletal muscle
Superoxides - metabolism
Wasting Syndrome - chemically induced
Wasting Syndrome - metabolism
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Summary:► Angiotensin II increases NADPH oxidase-derived ROS in skeletal muscle. ► Deletion of p47 phox prevents Ang II-induced skeletal muscle weight loss. ► Ang II-induced proteasome activity in skeletal muscle is prevented in p47 phox−/− mice. Angiotensin II (Ang II) causes skeletal muscle wasting via an increase in muscle catabolism. To determine whether the wasting effects of Ang II were related to its ability to increase NADPH oxidase-derived reactive oxygen species (ROS) we infused wild-type C57BL/6J or p47 phox −/− mice with vehicle or Ang II for 7 days. Superoxide production was increased 2.4-fold in the skeletal muscle of Ang II infused mice, and this increase was prevented in p47 phox −/− mice. Apocynin treatment prevented Ang II-induced superoxide production in skeletal muscle, consistent with Ang II increasing NADPH oxidase derived ROS. Ang II induced loss of body and skeletal muscle weight in C57BL/6J mice, whereas the reduction was significantly attenuated in p47 phox −/− animals. The reduction of skeletal muscle weight caused by Ang II was associated with an increase of proteasome activity, and this increase was completely prevented in the skeletal muscle of p47 phox −/− mice. In conclusion, Ang II-induced skeletal muscle wasting is in part dependent on NADPH oxidase derived ROS.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2011.04.122