Dextran sulphate sodium increases splenic Gr1+CD11b+ cells which accelerate recovery from colitis following intravenous transplantation

Summary While Gr1+CD11b+ cells are known to regulate immune responses and accumulate in most cancer tissues, the function of Gr1+CD11b+ cells in inflammation is poorly understood. We investigated the role of Gr1+CD11b+ cells in a dextran sulphate sodium (DSS)‐treated mouse model of ulcerative coliti...

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Bibliographic Details
Published in:Clinical and experimental immunology 2011-06, Vol.164 (3), p.417-427
Main Authors: Zhang, R., Ito, S., Nishio, N., Cheng, Z., Suzuki, H., Isobe, K.‐I.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animal Models
Animals
Biological and medical sciences
CD11b
CD11b Antigen - biosynthesis
Cell Count
Cell Differentiation
Cell Movement
Cell Transplantation
Cells, Cultured
Colitis, Ulcerative - chemically induced
Colitis, Ulcerative - immunology
Colitis, Ulcerative - physiopathology
Colitis, Ulcerative - therapy
Colon - pathology
Dextran Sulfate - administration & dosage
Disease Models, Animal
Disease Progression
DSS
Fundamental and applied biological sciences. Psychology
Gastroenterology. Liver. Pancreas. Abdomen
Gr‐1
Humans
Inflammatory bowel disease
Medical sciences
Mice
Mice, Inbred C57BL
myeloid‐lineage
Other diseases. Semiology
Receptors, Cell Surface - biosynthesis
Rodents
Sodium
Spleen - immunology
Spleen - metabolism
Spleen - pathology
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
ulcerative colitis
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Summary:Summary While Gr1+CD11b+ cells are known to regulate immune responses and accumulate in most cancer tissues, the function of Gr1+CD11b+ cells in inflammation is poorly understood. We investigated the role of Gr1+CD11b+ cells in a dextran sulphate sodium (DSS)‐treated mouse model of ulcerative colitis (UC). C57BL/6 mice were treated with 2% DSS in drinking water for 5 days. Disease progression and recovery were assessed by body weight, disease activity index score (DAI) score and colon length. Splenic Gr1+CD11b+ cell number was greatly increased during the recovery phase of DSS‐induced colitis. DSS‐derived splenic Gr1+CD11b+ cells were administered intravenously to recipient (C57BL/6) mice during the early phase of DSS treatment. The transplanted splenic DSS‐induced Gr1+CD11b+ cells improved DSS‐induced colitis and promoted efficient colonic mucosal healing. We found that the CD11b+ single positive cells increased in the course of DSS‐induced colitis in lamina propria. The transplantation of splenic Gr1+CD11b+ cells induced feedback suppression of myeloid‐lineage cell development. Namely, the transplantation of splenic Gr1+CD11b+ cells greatly suppressed the migration of CD11b+ single positive cells to the lamina propria. Further, transplantation of Gr‐1+CD11b+ cells greatly suppressed the increase of the same population, especially during the late phase of DSS colitis both in spleen and bone marrow.
ISSN:0009-9104
1365-2249
DOI:10.1111/j.1365-2249.2011.04374.x