Combined Effects of Surface Morphology and Mechanical Straining Magnitudes on the Differentiation of Mesenchymal Stem Cells without Using Biochemical Reagents

Existing studies examining the control of mesenchymal stem cell (MSC) differentiation into desired cell types have used a variety of biochemical reagents such as growth factors despite possible side effects. Recently, the roles of biomimetic microphysical environments have drawn much attention in th...

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Bibliographic Details
Published in:BioMed research international 2011-01, Vol.2011 (2011), p.1-9
Main Authors: Jang, Ji-Yeon, Lee, Shi Woo, Park, So Hee, Shin, Ji Won, Mun, ChiWoong, Kim, Su-Hyang, Kim, Dong Hwa, Shin, Jung-Woog
Format: Article
Language:English
Subjects:
Animals
Cell Differentiation - genetics
Cell Differentiation - physiology
Cell Survival - genetics
Cell Survival - physiology
Chemical tests and reagents
Gene Expression Profiling
Geometry
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - physiology
Metabolic disorders
Physiological aspects
Rabbits
Silicon wafers
Stains and staining (Microscopy)
Stem cells
Studies
Surface Properties
Tensile Strength
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Summary:Existing studies examining the control of mesenchymal stem cell (MSC) differentiation into desired cell types have used a variety of biochemical reagents such as growth factors despite possible side effects. Recently, the roles of biomimetic microphysical environments have drawn much attention in this field. We studied MSC differentiation and changes in gene expression in relation to osteoblast-like cell and smooth muscle-like cell type resulting from various microphysical environments, including differing magnitudes of tensile strain and substrate geometries for 8 days. In addition, we also investigated the residual effects of those selected microphysical environment factors on the differentiation by ceasing those factors for 3 days. The results of this study showed the effects of the strain magnitudes and surface geometries. However, the genes which are related to the same cell type showed different responses depending on the changes in strain magnitude and surface geometry. Also, different responses were observed three days after the straining was stopped. These data confirm that controlling microenvironments so that they mimic those in vivo contributes to the differentiation of MSCs into specific cell types. And duration of straining engagement was also found to play important roles along with surface geometry.
ISSN:1110-7243
2314-6133
1110-7251
2314-6141
DOI:10.1155/2011/860652