Convergence of the 26S proteasome and the REVOLUTA pathways in regulating inflorescence and floral meristem functions in Arabidopsis

The 26S proteasome is a large multisubunit proteolytic complex, regulating growth and development in eukaryotes by selective removal of short-lived regulatory proteins. Here, it is shown that the 26S proteasome and the transcription factor gene REVOLUTA (REV) act together in maintaining inflorescenc...

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Bibliographic Details
Published in:Journal of experimental botany 2011-01, Vol.62 (1), p.359-369
Main Authors: Zhang, Zhenzhen, Wang, Hua, Luo, Dexian, Zeng, Minhuan, Huang, Hai, Cui, Xiaofeng
Format: Article
Language:English
Subjects:
Arabidopsis - enzymology
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Biological and medical sciences
Calyx
Flowers
Flowers - enzymology
Flowers - genetics
Flowers - growth & development
Flowers - metabolism
Fundamental and applied biological sciences. Psychology
Gene expression regulation
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Inflorescence - enzymology
Inflorescence - genetics
Inflorescence - growth & development
Inflorescence - metabolism
Inflorescences
Meristem - enzymology
Meristem - genetics
Meristem - growth & development
Meristem - metabolism
Meristems
Phenotypes
Plant cells
Plants
Proteasome Endopeptidase Complex - genetics
Proteasome Endopeptidase Complex - metabolism
Protein Subunits - genetics
Protein Subunits - metabolism
RESEARCH PAPER
Research Papers
rev genes
Signal Transduction
Stem cells
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Summary:The 26S proteasome is a large multisubunit proteolytic complex, regulating growth and development in eukaryotes by selective removal of short-lived regulatory proteins. Here, it is shown that the 26S proteasome and the transcription factor gene REVOLUTA (REV) act together in maintaining inflorescence and floral meristem (IM and FM) functions. The characterization of a newly identified Arabidopsis mutant, designated ae4 (asymmetric leaves1/2 enhancer4), which carries a mutation in the gene encoding the 26S proteasome subunit, RPN2a, is reported. ae4 and rev have minor defects in phyllotaxy structure and meristem initiation, respectively, whereas ae4 rev demonstrated strong developmental defects. Compared with the rev single mutant, an increased percentage of ae4 rev plants exhibited abnormal vegetative shoot apical and axillary meristems. After flowering, ae4 rev first gave rise to a few normal-looking flowers, and then flowers with reduced numbers of all types of floral organs. In late reproductive development, instead of flowers, the ae4 rev IM produced numerous filamentous structures, which contained cells seen only in the floral organs, and then carpelloid organs. In situ hybridization revealed that expression of the WUSCHEL and CLAVATA3 genes was severely down-regulated or absent in the late appearing ae4 rev primordia, but the genes were strongly expressed in top-layer cells of inflorescence tips. Double mutant plants combining rev with other 26S proteasome subunit mutants, rpn1a and rpn9a, resembled ae4 rev, suggesting that the 26S proteasome might act as a whole in regulating IM and FM functions.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/erq277