Analysis of polyphenolic compounds and radical scavenging activity of four American Actaea species

A reversed-phase high-performance liquid chromatography (RP-HPLC) method with diode array detection has been developed for analysis of the major polyphenols in the roots and rhizomes of black cohosh (Actaea racemosa), an important botanical dietary supplement for women's health, and three close...

Full description

Saved in:
Bibliographic Details
Published in:Phytochemical analysis 2007-05, Vol.18 (3), p.219-228
Main Authors: Nuntanakom, P, Jiang, B, Yang, H, Cervantes-Cervantes, M, Kronenberg, F, Kennelly, E.J
Format: Article
Language:English
Subjects:
Actaea
Actaea - chemistry
Actaea pachypoda
Actaea podocarpa
Actaea racemosa
Actaea rubra
Actaea spp
antioxidant activity
antioxidants
black cohosh
Chromatography, High Pressure Liquid - methods
diode array detection
DPPH
Flavonoids - analysis
Flavonoids - chemistry
Flavonoids - pharmacology
free radical scavengers
Free Radical Scavengers - analysis
Free Radical Scavengers - chemistry
Free Radical Scavengers - pharmacology
HPLC
Molecular Biology
Phenols - analysis
Phenols - chemistry
Phenols - pharmacology
Plant Roots - chemistry
Polyphenols
quantitative analysis
Reproducibility of Results
reversed-phase high performance liquid chromatography
Rhizome - chemistry
Species Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A reversed-phase high-performance liquid chromatography (RP-HPLC) method with diode array detection has been developed for analysis of the major polyphenols in the roots and rhizomes of black cohosh (Actaea racemosa), an important botanical dietary supplement for women's health, and three closely related American Actaea species, A. rubra, A. pachypoda and A. podocarpa. The method was validated with respect to sensitivity, linearity, precision, accuracy and recovery. The total content of eight major polyphenols in the dried root and rhizome of the four species was determined to be from 0.36 to 2.92% (w/w). The antioxidant activities of Actaea extracts and polyphenolic compounds isolated from A. racemosa were evaluated on 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals scavenging assay. The radical scavenging activity of the Actaea extracts correlates to their polyphenolic composition. This validated HPLC method can be used to distinguish A. racemosa from the other major American Actaea species based on this study.
ISSN:0958-0344
1099-1565
DOI:10.1002/pca.975