Small RNA class transition from siRNA/piRNA to miRNA during pre-implantation mouse development

Recent studies showed that small interfering RNAs (siRNAs) and Piwi-interacting RNA (piRNA) in mammalian germ cells play important roles in retrotransposon silencing and gametogenesis. However, subsequent contribution of those small RNAs to early mammalian development remains poorly understood. We i...

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Bibliographic Details
Published in:Nucleic acids research 2010-08, Vol.38 (15), p.5141-5151
Main Authors: Ohnishi, Yusuke, Totoki, Yasushi, Toyoda, Atsushi, Watanabe, Toshiaki, Yamamoto, Yasuhiro, Tokunaga, Katsushi, Sakaki, Yoshiyuki, Sasaki, Hiroyuki, Hohjoh, Hirohiko
Format: Article
Language:English
Subjects:
Animals
Blastocyst - metabolism
Blastocyst Inner Cell Mass - metabolism
Cells, Cultured
DEAD-box RNA Helicases - antagonists & inhibitors
Development
Embryonic Development - genetics
Endoribonucleases - antagonists & inhibitors
Female
Mice
Mice, Inbred ICR
MicroRNAs - metabolism
Oocytes - metabolism
Retroelements
Ribonuclease III
RNA
RNA Interference
RNA, Small Interfering - genetics
RNA, Small Interfering - metabolism
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Summary:Recent studies showed that small interfering RNAs (siRNAs) and Piwi-interacting RNA (piRNA) in mammalian germ cells play important roles in retrotransposon silencing and gametogenesis. However, subsequent contribution of those small RNAs to early mammalian development remains poorly understood. We investigated the expression profiles of small RNAs in mouse metaphase II oocytes, 8-16-cell stage embryos, blastocysts and the pluripotent inner cell mass (ICM) using high-throughput pyrosequencing. Here, we show that during pre-implantation development a major small RNA class changes from retrotransposon-derived small RNAs containing siRNAs and piRNAs to zygotically synthesized microRNAs (miRNAs). Some siRNAs and piRNAs are transiently upregulated and directed against specific retrotransposon classes. We also identified miRNAs expression profiles characteristic of the ICM and trophectoderm (TE) cells. Taken together, our current study reveals a major reprogramming of functional small RNAs during early mouse development from oocyte to blastocyst.
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/gkq229