Angiotensin converting enzyme 2 (ACE2) activity in fetal calf serum: implications for cell culture research

Cell culture experiments often employ the use of culture media that contain fetal calf serum (FCS). The angiotensin peptides angiotensin II and angiotensin 1-7 have opposing effects with angiotensin converting enzyme 2 (ACE2) being the enzyme predominantly responsible for generating angiotensin 1-7...

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Bibliographic Details
Published in:Cytotechnology (Dordrecht) 2008-11, Vol.58 (3), p.119-126
Main Authors: Lubel, J. S, Herath, C. B, Velkoska, E, Casley, D. J, Burrell, L. M, Angus, P. W
Format: Article
Language:English
Subjects:
ACE2
Angiotensin
Angiotensin II
Angiotensin-converting enzyme 2
Antibodies
Antigens
Biochemistry
Biological and medical sciences
Biomedicine
Biotechnology
Brief Report
Cell culture
Chemistry
Chemistry and Materials Science
Culture media
Cytokines
Enzymatic activity
Enzymes
Fetal calf serum
Fundamental and applied biological sciences. Psychology
Influence
Media (culture)
Medical research
Penicillin
Peptides
Peptidyl-dipeptidase A
Polyethylene glycol
Variance analysis
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Summary:Cell culture experiments often employ the use of culture media that contain fetal calf serum (FCS). The angiotensin peptides angiotensin II and angiotensin 1-7 have opposing effects with angiotensin converting enzyme 2 (ACE2) being the enzyme predominantly responsible for generating angiotensin 1-7 from angiotensin II. The effect of FCS on angiotensin peptides has not previously been described. We have shown that FCS has ACE2 enzyme activity capable of degrading angiotensin II and generating angiotensin 1-7. Researchers should be aware that FCS possesses ACE2 activity and that heat-treating FCS to 56 °C only partially inhibits this enzyme activity, whereas heat-treating to 70 °C completely abolishes ACE2 activity.
ISSN:0920-9069
1573-0778
DOI:10.1007/s10616-009-9185-0