Establishment of three-dimensional cultures of human pancreatic duct epithelial cells

Three-dimensional (3D) cultures of epithelial cells offer singular advantages for studies of morphogenesis or the role of cancer genes in oncogenesis. In this study, as part of establishing a 3D culture system of pancreatic duct epithelial cells, we compared human pancreatic duct epithelial cells (H...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2007-07, Vol.358 (3), p.698-703
Main Authors: Gutierrez-Barrera, Angelica M., Menter, David G., Abbruzzese, James L., Reddy, Shrikanth A.G.
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Active Transport, Cell Nucleus
Akt
Cell Culture Techniques - methods
Cell Line, Tumor
CELL MEMBRANES
Cell Nucleus - metabolism
Collagen - pharmacology
CTMP
Cytoplasm - metabolism
Drug Combinations
Epithelial Cells - cytology
GENES
HUMAN POPULATIONS
Humans
Laminin - pharmacology
MCF-10A
Microscopy, Fluorescence
MORPHOGENESIS
NEOPLASMS
Nuclear localization
PANCREAS
Pancreatic cancer
Pancreatic ductal epithelial cells
Pancreatic Ducts - cytology
Pancreatic Ducts - pathology
Pancreatic Neoplasms - metabolism
Pancreatic Neoplasms - pathology
PHENOBARBITAL
Phosphorylation
PROTEINS
Proteoglycans - pharmacology
Proto-Oncogene Proteins c-akt - metabolism
Signal Transduction
Three-dimensional culture
Tumor Cells, Cultured - cytology
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Summary:Three-dimensional (3D) cultures of epithelial cells offer singular advantages for studies of morphogenesis or the role of cancer genes in oncogenesis. In this study, as part of establishing a 3D culture system of pancreatic duct epithelial cells, we compared human pancreatic duct epithelial cells (HPDE-E6E7) with pancreatic cancer cell lines. Our results show, that in contrast to cancer cells, HPDE-E6E7 organized into spheroids with what appeared to be apical and basal membranes and a luminal space. Immunostaining experiments indicated that protein kinase Akt was phosphorylated (Ser473) and CTMP, a negative Akt regulator, was expressed in both HPDE-E6E7 and cancer cells. However, a nuclear pool of CTMP was detectable in HPDE-E6E7 cells that showed a dynamic concentrated expression pattern, a feature that further distinguished HPDE-E637 cells from cancer cells. Collectively, these data suggest that 3D cultures of HPDE-E6E7 cells are useful for investigating signaling and morphological abnormalities in pancreatic cancer cells.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.04.166