Comparative analysis of in vivo interactions between Rev1 protein and other Y-family DNA polymerases in animals and yeasts

Eukaryotes are endowed with multiple specialized DNA polymerases, some (if not all) of which are believed to play important roles in the tolerance of base damage during DNA replication. Among these DNA polymerases, Rev1 protein (a deoxycytidyl transferase) from vertebrates interacts with several oth...

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Bibliographic Details
Published in:DNA repair 2008-03, Vol.7 (3), p.439-451
Main Authors: Kosarek, J. Nicole, Woodruff, Rachel V., Rivera-Begeman, Amanda, Guo, Caixia, D'Souza, Sanjay, Koonin, Eugene V., Walker, Graham C., Friedberg, Errol C.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
beta-Galactosidase - metabolism
Caenorhabditis elegans
Caenorhabditis elegans - metabolism
DNA-Directed DNA Polymerase - metabolism
Drosophila melanogaster
Drosophila melanogaster - metabolism
Immunoblotting
Immunoprecipitation
Mice
Molecular Sequence Data
Nematoda
Nucleotidyltransferases - metabolism
Phylogeny
Polymerase η
Polymerase ι
Polymerase κ
Protein–protein interactions
Rev1
Saccharomyces cerevisiae
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - metabolism
Schizosaccharomyces - metabolism
Schizosaccharomyces pombe
Sequence Homology, Amino Acid
TLS
Two-Hybrid System Techniques
Y-family of DNA polymerases
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Summary:Eukaryotes are endowed with multiple specialized DNA polymerases, some (if not all) of which are believed to play important roles in the tolerance of base damage during DNA replication. Among these DNA polymerases, Rev1 protein (a deoxycytidyl transferase) from vertebrates interacts with several other specialized polymerases via a highly conserved C-terminal region. The present studies assessed whether these interactions are retained in more experimentally tractable model systems, including yeasts, flies, and the nematode C. elegans. We observed a physical interaction between Rev1 protein and other Y-family polymerases in the fruit fly Drosophila melanogaster. However, despite the fact that the C-terminal region of Drosophila and yeast Rev1 are conserved from vertebrates to a similar extent, such interactions were not observed in Saccharomyces cerevisiae or Schizosaccharomyces pombe. With respect to regions in specialized DNA polymerases that are required for interaction with Rev1, we find predicted disorder to be an underlying structural commonality. The results of this study suggest that special consideration should be exercised when making mechanistic extrapolations regarding translesion DNA synthesis from one eukaryotic system to another.
ISSN:1568-7864
1568-7856
DOI:10.1016/j.dnarep.2007.11.016