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Unusual nuclear structures in meiotic prophase of fission yeast: a cytological analysis
Earlier results from sectioned nuclei indicating that Schizosaccharomyces pombe does not develop a classical tripartite synaptonemal complex (SC) during meiotic prophase are confirmed by spreading of whole nuclei. The linear elements appearing during prophase I resemble the axial cores (SC precursor...
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| Published in: | The Journal of cell biology 1993-04, Vol.121 (2), p.241-256 |
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| container_end_page | 256 |
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| container_title | The Journal of cell biology |
| container_volume | 121 |
| creator | Bahler, J. (University of Bern, Bern, Switzerland) Wyler, T Loidl, J Kohli, J |
| description | Earlier results from sectioned nuclei indicating that Schizosaccharomyces pombe does not develop a classical tripartite synaptonemal complex (SC) during meiotic prophase are confirmed by spreading of whole nuclei. The linear elements appearing during prophase I resemble the axial cores (SC precursors) of other organisms. The number of linear elements in haploid, diploid, and tetraploid strains is always higher than the chromosome number, implying that they are not formed continuously along the chromosomes. Time course experiments reveal that the elements appear after DNA replication and form networks and bundles. Later they separate and approximately 24 individual elements with a total length of 34 micrometers are observed before degradation and meiotic divisions. Parallel staining of DNA reveals changes in nuclear shape during meiotic prophase. Strains with a mei4 mutation are blocked at a late prophase stage. In serial sections we additionally observed a constant arrangement of the spindle pole body, the nucleolus, and the presumptive centromere cluster. Thus, S. pombe manages to recombine and segregate its chromosomes without SC. This might correlate with the absence of crossover interference. We propose a mechanism for chromosome pairing with initial recognition of the homologs at the centromeres and suggest functions of the linear elements in preparation of the chromosomes for meiosis I disjunction. With the spreading technique combined genetic, molecular, and cytological approaches become feasible in S. pombe. This provides an opportunity to study essential meiotic functions in the absence of SCs which may help to clarify the significance of the SC and its components for meiotic chromosome structure and function |
| doi_str_mv | 10.1083/jcb.121.2.241 |
| format | article |
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(University of Bern, Bern, Switzerland) ; Wyler, T ; Loidl, J ; Kohli, J</creator><creatorcontrib>Bahler, J. (University of Bern, Bern, Switzerland) ; Wyler, T ; Loidl, J ; Kohli, J</creatorcontrib><description>Earlier results from sectioned nuclei indicating that Schizosaccharomyces pombe does not develop a classical tripartite synaptonemal complex (SC) during meiotic prophase are confirmed by spreading of whole nuclei. The linear elements appearing during prophase I resemble the axial cores (SC precursors) of other organisms. The number of linear elements in haploid, diploid, and tetraploid strains is always higher than the chromosome number, implying that they are not formed continuously along the chromosomes. Time course experiments reveal that the elements appear after DNA replication and form networks and bundles. Later they separate and approximately 24 individual elements with a total length of 34 micrometers are observed before degradation and meiotic divisions. Parallel staining of DNA reveals changes in nuclear shape during meiotic prophase. Strains with a mei4 mutation are blocked at a late prophase stage. In serial sections we additionally observed a constant arrangement of the spindle pole body, the nucleolus, and the presumptive centromere cluster. Thus, S. pombe manages to recombine and segregate its chromosomes without SC. This might correlate with the absence of crossover interference. We propose a mechanism for chromosome pairing with initial recognition of the homologs at the centromeres and suggest functions of the linear elements in preparation of the chromosomes for meiosis I disjunction. With the spreading technique combined genetic, molecular, and cytological approaches become feasible in S. pombe. This provides an opportunity to study essential meiotic functions in the absence of SCs which may help to clarify the significance of the SC and its components for meiotic chromosome structure and function</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.121.2.241</identifier><identifier>PMID: 8468345</identifier><identifier>CODEN: JCLBA3</identifier><language>eng</language><publisher>New York, NY: Rockefeller University Press</publisher><subject>APAREAMIENTO CROMOSOMICO ; APPARIEMENT CHROMOSOMIQUE ; Biological and medical sciences ; Cell nucleus ; Cell Nucleus - ultrastructure ; Cells ; Cellular biology ; Centromeres ; Chromatin ; Chromatin. Chromosome ; CHROMOSOME ; Chromosomes ; CITOLOGIA ; CROMOSOMAS ; Crossing Over, Genetic ; Cytological Techniques ; CYTOLOGIE ; Deoxyribonucleic acid ; Diploidy ; DNA ; ENDOMYCETALES ; Fundamental and applied biological sciences. Psychology ; Genes ; Haploidy ; MEIOSE ; MEIOSIS ; Models, Biological ; Molecular and cellular biology ; Molecular genetics ; Prophase ; RECOMBINACION ; RECOMBINAISON ; Schizosaccharomyces - cytology ; Schizosaccharomyces - ultrastructure ; Schizosaccharomyces pombe ; Synaptonemal Complex ; Yeasts</subject><ispartof>The Journal of cell biology, 1993-04, Vol.121 (2), p.241-256</ispartof><rights>Copyright 1993 The Rockefeller University Press</rights><rights>1993 INIST-CNRS</rights><rights>Copyright Rockefeller University Press Apr 1993</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c575t-723ab27646fe6f453e347259c3ab7d4023eb15e16c976c9e00efa66201ea82ec3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2200093/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2200093/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,733,786,790,891,27957,27958,53827,53829</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4744443$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8468345$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bahler, J. (University of Bern, Bern, Switzerland)</creatorcontrib><creatorcontrib>Wyler, T</creatorcontrib><creatorcontrib>Loidl, J</creatorcontrib><creatorcontrib>Kohli, J</creatorcontrib><title>Unusual nuclear structures in meiotic prophase of fission yeast: a cytological analysis</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>Earlier results from sectioned nuclei indicating that Schizosaccharomyces pombe does not develop a classical tripartite synaptonemal complex (SC) during meiotic prophase are confirmed by spreading of whole nuclei. The linear elements appearing during prophase I resemble the axial cores (SC precursors) of other organisms. The number of linear elements in haploid, diploid, and tetraploid strains is always higher than the chromosome number, implying that they are not formed continuously along the chromosomes. Time course experiments reveal that the elements appear after DNA replication and form networks and bundles. Later they separate and approximately 24 individual elements with a total length of 34 micrometers are observed before degradation and meiotic divisions. Parallel staining of DNA reveals changes in nuclear shape during meiotic prophase. Strains with a mei4 mutation are blocked at a late prophase stage. In serial sections we additionally observed a constant arrangement of the spindle pole body, the nucleolus, and the presumptive centromere cluster. Thus, S. pombe manages to recombine and segregate its chromosomes without SC. This might correlate with the absence of crossover interference. We propose a mechanism for chromosome pairing with initial recognition of the homologs at the centromeres and suggest functions of the linear elements in preparation of the chromosomes for meiosis I disjunction. With the spreading technique combined genetic, molecular, and cytological approaches become feasible in S. pombe. This provides an opportunity to study essential meiotic functions in the absence of SCs which may help to clarify the significance of the SC and its components for meiotic chromosome structure and function</description><subject>APAREAMIENTO CROMOSOMICO</subject><subject>APPARIEMENT CHROMOSOMIQUE</subject><subject>Biological and medical sciences</subject><subject>Cell nucleus</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Cells</subject><subject>Cellular biology</subject><subject>Centromeres</subject><subject>Chromatin</subject><subject>Chromatin. Chromosome</subject><subject>CHROMOSOME</subject><subject>Chromosomes</subject><subject>CITOLOGIA</subject><subject>CROMOSOMAS</subject><subject>Crossing Over, Genetic</subject><subject>Cytological Techniques</subject><subject>CYTOLOGIE</subject><subject>Deoxyribonucleic acid</subject><subject>Diploidy</subject><subject>DNA</subject><subject>ENDOMYCETALES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Haploidy</subject><subject>MEIOSE</subject><subject>MEIOSIS</subject><subject>Models, Biological</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Prophase</subject><subject>RECOMBINACION</subject><subject>RECOMBINAISON</subject><subject>Schizosaccharomyces - cytology</subject><subject>Schizosaccharomyces - ultrastructure</subject><subject>Schizosaccharomyces pombe</subject><subject>Synaptonemal Complex</subject><subject>Yeasts</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNqFkcuLUzEUh4MoYx1duhGFIOLu1rzT60KQwRcMuNDiMqSZczsptzednHuF_vee0lIfGwMhkPPxy8n5GHsqxVyKhX6zSau5VHKu5srIe2wmrRHNQhpxn82EULJprbIP2SPEjRDCeKMv2MXCuIU2dsZ-LIcJp9jzYUo9xMpxrFMapwrI88C3kMuYE9_VsruNCLx0vMuIuQx8DxHHtzzytB9LX9Y5UUwcYr_HjI_Zgy72CE9O5yVbfvzw_epzc_3105er99dNst6OjVc6rpR3xnXgOmM1aOOVbRNd-xsjlIaVtCBdaj1tEAK66JwSEuJCQdKX7N0xdzettnCTYBhr7MOu5m2s-1BiDn9Xhnwb1uVnUIqm0WoKeH0KqOVuAhzDNmOCvo8DlAmDt86ZVqn_gtJ57SmQwJf_gJsyVZoLBiW9FJYmT1BzhFItiBW6c8tShIPXQF4DeQ0qkFfiX_z5zzN9Ekn1V6d6RPLQ1TikjGeMtNM69Pb8iG1wLPX3m06SjkPKs2O5iyXEdaWE5bfWSGPaVv8CIWG9iA</recordid><startdate>19930401</startdate><enddate>19930401</enddate><creator>Bahler, J. (University of Bern, Bern, Switzerland)</creator><creator>Wyler, T</creator><creator>Loidl, J</creator><creator>Kohli, J</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19930401</creationdate><title>Unusual nuclear structures in meiotic prophase of fission yeast: a cytological analysis</title><author>Bahler, J. (University of Bern, Bern, Switzerland) ; Wyler, T ; Loidl, J ; Kohli, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c575t-723ab27646fe6f453e347259c3ab7d4023eb15e16c976c9e00efa66201ea82ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>APAREAMIENTO CROMOSOMICO</topic><topic>APPARIEMENT CHROMOSOMIQUE</topic><topic>Biological and medical sciences</topic><topic>Cell nucleus</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Cells</topic><topic>Cellular biology</topic><topic>Centromeres</topic><topic>Chromatin</topic><topic>Chromatin. Chromosome</topic><topic>CHROMOSOME</topic><topic>Chromosomes</topic><topic>CITOLOGIA</topic><topic>CROMOSOMAS</topic><topic>Crossing Over, Genetic</topic><topic>Cytological Techniques</topic><topic>CYTOLOGIE</topic><topic>Deoxyribonucleic acid</topic><topic>Diploidy</topic><topic>DNA</topic><topic>ENDOMYCETALES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Haploidy</topic><topic>MEIOSE</topic><topic>MEIOSIS</topic><topic>Models, Biological</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Prophase</topic><topic>RECOMBINACION</topic><topic>RECOMBINAISON</topic><topic>Schizosaccharomyces - cytology</topic><topic>Schizosaccharomyces - ultrastructure</topic><topic>Schizosaccharomyces pombe</topic><topic>Synaptonemal Complex</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bahler, J. (University of Bern, Bern, Switzerland)</creatorcontrib><creatorcontrib>Wyler, T</creatorcontrib><creatorcontrib>Loidl, J</creatorcontrib><creatorcontrib>Kohli, J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bahler, J. (University of Bern, Bern, Switzerland)</au><au>Wyler, T</au><au>Loidl, J</au><au>Kohli, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Unusual nuclear structures in meiotic prophase of fission yeast: a cytological analysis</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>1993-04-01</date><risdate>1993</risdate><volume>121</volume><issue>2</issue><spage>241</spage><epage>256</epage><pages>241-256</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><coden>JCLBA3</coden><notes>9414499</notes><notes>F30</notes><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-1</notes><notes>content type line 23</notes><notes>ObjectType-Article-1</notes><notes>ObjectType-Feature-2</notes><abstract>Earlier results from sectioned nuclei indicating that Schizosaccharomyces pombe does not develop a classical tripartite synaptonemal complex (SC) during meiotic prophase are confirmed by spreading of whole nuclei. The linear elements appearing during prophase I resemble the axial cores (SC precursors) of other organisms. The number of linear elements in haploid, diploid, and tetraploid strains is always higher than the chromosome number, implying that they are not formed continuously along the chromosomes. Time course experiments reveal that the elements appear after DNA replication and form networks and bundles. Later they separate and approximately 24 individual elements with a total length of 34 micrometers are observed before degradation and meiotic divisions. Parallel staining of DNA reveals changes in nuclear shape during meiotic prophase. Strains with a mei4 mutation are blocked at a late prophase stage. In serial sections we additionally observed a constant arrangement of the spindle pole body, the nucleolus, and the presumptive centromere cluster. Thus, S. pombe manages to recombine and segregate its chromosomes without SC. This might correlate with the absence of crossover interference. We propose a mechanism for chromosome pairing with initial recognition of the homologs at the centromeres and suggest functions of the linear elements in preparation of the chromosomes for meiosis I disjunction. With the spreading technique combined genetic, molecular, and cytological approaches become feasible in S. pombe. This provides an opportunity to study essential meiotic functions in the absence of SCs which may help to clarify the significance of the SC and its components for meiotic chromosome structure and function</abstract><cop>New York, NY</cop><pub>Rockefeller University Press</pub><pmid>8468345</pmid><doi>10.1083/jcb.121.2.241</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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| source | Open Access: PubMed Central; Alma/SFX Local Collection |
| subjects | APAREAMIENTO CROMOSOMICO APPARIEMENT CHROMOSOMIQUE Biological and medical sciences Cell nucleus Cell Nucleus - ultrastructure Cells Cellular biology Centromeres Chromatin Chromatin. Chromosome CHROMOSOME Chromosomes CITOLOGIA CROMOSOMAS Crossing Over, Genetic Cytological Techniques CYTOLOGIE Deoxyribonucleic acid Diploidy DNA ENDOMYCETALES Fundamental and applied biological sciences. Psychology Genes Haploidy MEIOSE MEIOSIS Models, Biological Molecular and cellular biology Molecular genetics Prophase RECOMBINACION RECOMBINAISON Schizosaccharomyces - cytology Schizosaccharomyces - ultrastructure Schizosaccharomyces pombe Synaptonemal Complex Yeasts |
| title | Unusual nuclear structures in meiotic prophase of fission yeast: a cytological analysis |
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