BED1, a gene encoding a galactosyltransferase homologue, is required for polarized growth and efficient bud emergence in Saccharomyces cerevisiae

The ellipsoidal shape of the yeast Saccharomyces cerevisiae is the result of successive isotropic/apical growth switches that are regulated in a cell cycle-dependent manner. It is thought that growth polarity is governed by the remodeling of the actin cytoskeleton that is itself under the control of...

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Bibliographic Details
Published in:The Journal of cell biology 1996, Vol.132 (1/2), p.137-151
Main Authors: Mondésert, Guillaume, Reed, Steven I.
Format: Article
Language:English
Subjects:
Actins
Actins - isolation & purification
Amino Acid Sequence
Amino acids
Base Sequence
Cell cycle
Cell division
Cell growth
Cell Polarity - genetics
Cell walls
Cells
cellule
celulas
chemical composition
Chitin - isolation & purification
Cloning, Molecular
composicion quimica
composition chimique
crecimiento
croissance
Cyclins - genetics
Cyclins - metabolism
Daughter cells
division cellulaire
division celular
endoplasmic reticulum
Endoplasmic Reticulum - chemistry
Galactosyltransferases - genetics
gene
Generally accepted auditing standards
Genes
Genes, Fungal
Genes, Lethal
Genetic mutation
glicosiltransferasas
glycosyltransferase
glycosyltransferases
growth
Molecular Sequence Data
Morphogenesis - genetics
mutant
mutantes
mutants
nucleotide sequence
Orotidine-5'-Phosphate Decarboxylase - genetics
Proteins
reticulo endoplasmatico
reticulum endoplasmique
saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
secuencia nucleica
Sequence Analysis, DNA
Sequence Homology, Amino Acid
sequence nucleique
Yeast
Yeasts
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Summary:The ellipsoidal shape of the yeast Saccharomyces cerevisiae is the result of successive isotropic/apical growth switches that are regulated in a cell cycle-dependent manner. It is thought that growth polarity is governed by the remodeling of the actin cytoskeleton that is itself under the control of the cell cycle machinery. The cell cycle and the morphogenesis cycle are tightly coupled and it has been recently suggested that a morphogenesis/polarity checkpoint control monitors bud emergence in order to maintain the coupling of these two events (Lew, D.J., and S.I. Reed. 1995. J. Cell Biol. 129:739-749). During a screen based on the inability of cells impaired in the budding process to survive when the morphogenesis checkpoint control is abolished, we identified and characterized BED1, a new gene that is required for efficient budding. Cells carrying a disrupted allele of BED1 no longer have the wild-type ellipsoidal shape characteristic of S. cerevisiae, are larger than wild-type cells, are deficient in bud emergence, and depend upon an intact morphogenesis checkpoint control to survive. These cells show defects in polarized growth despite the fact that the actin cytoskeleton appears normal. Our results suggest that Bed1 is a type II membrane protein localized in the endoplasmic reticulum. BED1 is significantly homologous to gma12+, a S. pombe gene coding for an alpha-1,2-galactosyltransferase, suggesting that glycosylation of specific proteins or lipids could be important for signaling in the switch to polarized growth and in bud emergence.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.132.1.137