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Induction of a virus-specific effector-memory CD4+ T cell response by attenuated SIV infection
We investigated simian immunodeficiency virus (SIV)-specific CD4+ T cell responses in rhesus macaques chronically infected with attenuated or pathogenic SIV strains. Analysis of SIVDeltanef-infected animals revealed a relatively high frequency of SIV-specific CD4+ T cells representing 4-10% of all C...
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| Published in: | The Journal of experimental medicine 2006-11, Vol.203 (12), p.2661-2672 |
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| container_title | The Journal of experimental medicine |
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| creator | Gauduin, Marie-Claire Yu, Yi Barabasz, Amy Carville, Angela Piatak, Mike Lifson, Jeffrey D Desrosiers, Ronald C Johnson, R Paul |
| description | We investigated simian immunodeficiency virus (SIV)-specific CD4+ T cell responses in rhesus macaques chronically infected with attenuated or pathogenic SIV strains. Analysis of SIVDeltanef-infected animals revealed a relatively high frequency of SIV-specific CD4+ T cells representing 4-10% of all CD4+ T lymphocytes directed against multiple SIV proteins. Gag-specific CD4+ T cells in wild-type SIV-infected animals were 5-10-fold lower in frequency and inversely correlated with the level of plasma viremia. SIV-specific CD4+ cells from SIVDeltanef animals were predominantly CD27-CD28-CD45RAlowCCR7-CCR5-, consistent with an effector-memory subset, and included a fully differentiated CD45RA+CCR7- subpopulation. In contrast, SIV-specific CD4+ T cells from SIV-infected animals were mostly CD27+CD28+CD45RA-CCR7+CCR5+, consistent with an early central memory phenotype. The CD45RA+CCR7-CD4+ subset from SIVDeltanef animals was highly enriched for effector CD4+ T cells, as indicated by the perforin expression and up-regulation of the lysosomal membrane protein CD107a after SIV Gag stimulation. SIV-specific CD4+ T cells in attenuated SIV-infected animals were increased in frequency in bronchioalveolar lavage and decreased in lymph nodes, consistent with an effector-memory T cell population. The ability of SIVDeltanef to induce a high frequency virus-specific CD4+ T cell response with direct effector function may play a key role in protective immunity produced by vaccination with attenuated SIV strains. |
| doi_str_mv | 10.1084/jem.20060134 |
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Analysis of SIVDeltanef-infected animals revealed a relatively high frequency of SIV-specific CD4+ T cells representing 4-10% of all CD4+ T lymphocytes directed against multiple SIV proteins. Gag-specific CD4+ T cells in wild-type SIV-infected animals were 5-10-fold lower in frequency and inversely correlated with the level of plasma viremia. SIV-specific CD4+ cells from SIVDeltanef animals were predominantly CD27-CD28-CD45RAlowCCR7-CCR5-, consistent with an effector-memory subset, and included a fully differentiated CD45RA+CCR7- subpopulation. In contrast, SIV-specific CD4+ T cells from SIV-infected animals were mostly CD27+CD28+CD45RA-CCR7+CCR5+, consistent with an early central memory phenotype. The CD45RA+CCR7-CD4+ subset from SIVDeltanef animals was highly enriched for effector CD4+ T cells, as indicated by the perforin expression and up-regulation of the lysosomal membrane protein CD107a after SIV Gag stimulation. SIV-specific CD4+ T cells in attenuated SIV-infected animals were increased in frequency in bronchioalveolar lavage and decreased in lymph nodes, consistent with an effector-memory T cell population. The ability of SIVDeltanef to induce a high frequency virus-specific CD4+ T cell response with direct effector function may play a key role in protective immunity produced by vaccination with attenuated SIV strains.</description><identifier>ISSN: 0022-1007</identifier><identifier>EISSN: 1540-9538</identifier><identifier>DOI: 10.1084/jem.20060134</identifier><identifier>PMID: 17116733</identifier><language>eng</language><publisher>United States: The Rockefeller University Press</publisher><subject>Animals ; Antibody Specificity ; CD4-Positive T-Lymphocytes - immunology ; CD4-Positive T-Lymphocytes - virology ; Chronic Disease ; Immunologic Memory - immunology ; Immunophenotyping ; Lymphocyte Activation - immunology ; Macaca mulatta ; Simian Acquired Immunodeficiency Syndrome - immunology ; Simian Acquired Immunodeficiency Syndrome - virology ; Simian Immunodeficiency Virus - immunology</subject><ispartof>The Journal of experimental medicine, 2006-11, Vol.203 (12), p.2661-2672</ispartof><rights>Copyright © 2006, The Rockefeller University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-98d92948fd4f87742398ba0a58523f842dafb836d0f8303dd739f171e7a0c1bf3</citedby><cites>FETCH-LOGICAL-c382t-98d92948fd4f87742398ba0a58523f842dafb836d0f8303dd739f171e7a0c1bf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118155/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118155/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,733,786,790,891,27957,27958,53827,53829</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17116733$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gauduin, Marie-Claire</creatorcontrib><creatorcontrib>Yu, Yi</creatorcontrib><creatorcontrib>Barabasz, Amy</creatorcontrib><creatorcontrib>Carville, Angela</creatorcontrib><creatorcontrib>Piatak, Mike</creatorcontrib><creatorcontrib>Lifson, Jeffrey D</creatorcontrib><creatorcontrib>Desrosiers, Ronald C</creatorcontrib><creatorcontrib>Johnson, R Paul</creatorcontrib><title>Induction of a virus-specific effector-memory CD4+ T cell response by attenuated SIV infection</title><title>The Journal of experimental medicine</title><addtitle>J Exp Med</addtitle><description>We investigated simian immunodeficiency virus (SIV)-specific CD4+ T cell responses in rhesus macaques chronically infected with attenuated or pathogenic SIV strains. Analysis of SIVDeltanef-infected animals revealed a relatively high frequency of SIV-specific CD4+ T cells representing 4-10% of all CD4+ T lymphocytes directed against multiple SIV proteins. Gag-specific CD4+ T cells in wild-type SIV-infected animals were 5-10-fold lower in frequency and inversely correlated with the level of plasma viremia. SIV-specific CD4+ cells from SIVDeltanef animals were predominantly CD27-CD28-CD45RAlowCCR7-CCR5-, consistent with an effector-memory subset, and included a fully differentiated CD45RA+CCR7- subpopulation. In contrast, SIV-specific CD4+ T cells from SIV-infected animals were mostly CD27+CD28+CD45RA-CCR7+CCR5+, consistent with an early central memory phenotype. The CD45RA+CCR7-CD4+ subset from SIVDeltanef animals was highly enriched for effector CD4+ T cells, as indicated by the perforin expression and up-regulation of the lysosomal membrane protein CD107a after SIV Gag stimulation. SIV-specific CD4+ T cells in attenuated SIV-infected animals were increased in frequency in bronchioalveolar lavage and decreased in lymph nodes, consistent with an effector-memory T cell population. The ability of SIVDeltanef to induce a high frequency virus-specific CD4+ T cell response with direct effector function may play a key role in protective immunity produced by vaccination with attenuated SIV strains.</description><subject>Animals</subject><subject>Antibody Specificity</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>CD4-Positive T-Lymphocytes - virology</subject><subject>Chronic Disease</subject><subject>Immunologic Memory - immunology</subject><subject>Immunophenotyping</subject><subject>Lymphocyte Activation - immunology</subject><subject>Macaca mulatta</subject><subject>Simian Acquired Immunodeficiency Syndrome - immunology</subject><subject>Simian Acquired Immunodeficiency Syndrome - virology</subject><subject>Simian Immunodeficiency Virus - immunology</subject><issn>0022-1007</issn><issn>1540-9538</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNpVkU1P3DAQhq2qqCyUW8-VT72UwPgjiXNBqhbarrQShy4csRx7XIw28WInSPvvyYothdMc5pl3ZvQQ8oXBGQMlzx-wO-MAFTAhP5AZKyUUTSnURzID4LxgAPUhOcr5AYBJWVafyCGrGatqIWbkbtG70Q4h9jR6auhTSGMu8gZt8MFS9B7tEFPRYRfTls4v5Xe6ohbXa5owb2KfkbZbaoYB-9EM6OifxS0N_W5sCv1MDrxZZzzZ12Ny8_NqNf9dLK9_LeY_loUVig9Fo1zDG6m8k17VteSiUa0BU6qSC68kd8a3SlQOvBIgnKtF46cnsDZgWevFMbl4yd2MbYfOYj8ks9abFDqTtjqaoN93-nCv_8YnzRlTrCyngG_7gBQfR8yD7kLevWl6jGPWlWIN51xO4OkLaFPMOaF_XcJA74ToSYj-J2TCv7497D-8NyCeARx5h1I</recordid><startdate>20061127</startdate><enddate>20061127</enddate><creator>Gauduin, Marie-Claire</creator><creator>Yu, Yi</creator><creator>Barabasz, Amy</creator><creator>Carville, Angela</creator><creator>Piatak, Mike</creator><creator>Lifson, Jeffrey D</creator><creator>Desrosiers, Ronald C</creator><creator>Johnson, R Paul</creator><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20061127</creationdate><title>Induction of a virus-specific effector-memory CD4+ T cell response by attenuated SIV infection</title><author>Gauduin, Marie-Claire ; Yu, Yi ; Barabasz, Amy ; Carville, Angela ; Piatak, Mike ; Lifson, Jeffrey D ; Desrosiers, Ronald C ; Johnson, R Paul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-98d92948fd4f87742398ba0a58523f842dafb836d0f8303dd739f171e7a0c1bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Antibody Specificity</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>CD4-Positive T-Lymphocytes - virology</topic><topic>Chronic Disease</topic><topic>Immunologic Memory - immunology</topic><topic>Immunophenotyping</topic><topic>Lymphocyte Activation - immunology</topic><topic>Macaca mulatta</topic><topic>Simian Acquired Immunodeficiency Syndrome - immunology</topic><topic>Simian Acquired Immunodeficiency Syndrome - virology</topic><topic>Simian Immunodeficiency Virus - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gauduin, Marie-Claire</creatorcontrib><creatorcontrib>Yu, Yi</creatorcontrib><creatorcontrib>Barabasz, Amy</creatorcontrib><creatorcontrib>Carville, Angela</creatorcontrib><creatorcontrib>Piatak, Mike</creatorcontrib><creatorcontrib>Lifson, Jeffrey D</creatorcontrib><creatorcontrib>Desrosiers, Ronald C</creatorcontrib><creatorcontrib>Johnson, R Paul</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of experimental medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gauduin, Marie-Claire</au><au>Yu, Yi</au><au>Barabasz, Amy</au><au>Carville, Angela</au><au>Piatak, Mike</au><au>Lifson, Jeffrey D</au><au>Desrosiers, Ronald C</au><au>Johnson, R Paul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of a virus-specific effector-memory CD4+ T cell response by attenuated SIV infection</atitle><jtitle>The Journal of experimental medicine</jtitle><addtitle>J Exp Med</addtitle><date>2006-11-27</date><risdate>2006</risdate><volume>203</volume><issue>12</issue><spage>2661</spage><epage>2672</epage><pages>2661-2672</pages><issn>0022-1007</issn><eissn>1540-9538</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>M.-C. Gauduin's present address is Dept. of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, TX 78245.</notes><notes>CORRESPONDENCE Marie-Claire Gauduin: mcgauduin@sfbr.org</notes><notes>Abbreviations used: BAL, bronchoalveolar lavage; ICS, intracellular cytokine staining; SEA, staphylococcal enterotoxin A; SEB, staphylococcal enterotoxin B; SIV, simian immunodeficiency virus.</notes><abstract>We investigated simian immunodeficiency virus (SIV)-specific CD4+ T cell responses in rhesus macaques chronically infected with attenuated or pathogenic SIV strains. Analysis of SIVDeltanef-infected animals revealed a relatively high frequency of SIV-specific CD4+ T cells representing 4-10% of all CD4+ T lymphocytes directed against multiple SIV proteins. Gag-specific CD4+ T cells in wild-type SIV-infected animals were 5-10-fold lower in frequency and inversely correlated with the level of plasma viremia. SIV-specific CD4+ cells from SIVDeltanef animals were predominantly CD27-CD28-CD45RAlowCCR7-CCR5-, consistent with an effector-memory subset, and included a fully differentiated CD45RA+CCR7- subpopulation. In contrast, SIV-specific CD4+ T cells from SIV-infected animals were mostly CD27+CD28+CD45RA-CCR7+CCR5+, consistent with an early central memory phenotype. The CD45RA+CCR7-CD4+ subset from SIVDeltanef animals was highly enriched for effector CD4+ T cells, as indicated by the perforin expression and up-regulation of the lysosomal membrane protein CD107a after SIV Gag stimulation. SIV-specific CD4+ T cells in attenuated SIV-infected animals were increased in frequency in bronchioalveolar lavage and decreased in lymph nodes, consistent with an effector-memory T cell population. The ability of SIVDeltanef to induce a high frequency virus-specific CD4+ T cell response with direct effector function may play a key role in protective immunity produced by vaccination with attenuated SIV strains.</abstract><cop>United States</cop><pub>The Rockefeller University Press</pub><pmid>17116733</pmid><doi>10.1084/jem.20060134</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of experimental medicine, 2006-11, Vol.203 (12), p.2661-2672 |
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| source | Open Access: PubMed Central |
| subjects | Animals Antibody Specificity CD4-Positive T-Lymphocytes - immunology CD4-Positive T-Lymphocytes - virology Chronic Disease Immunologic Memory - immunology Immunophenotyping Lymphocyte Activation - immunology Macaca mulatta Simian Acquired Immunodeficiency Syndrome - immunology Simian Acquired Immunodeficiency Syndrome - virology Simian Immunodeficiency Virus - immunology |
| title | Induction of a virus-specific effector-memory CD4+ T cell response by attenuated SIV infection |
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