Defects in lamin B1 expression or processing affect interphase chromosome position and gene expression

Radial organization of nuclei with peripheral gene-poor chromosomes and central gene-rich chromosomes is common and could depend on the nuclear boundary as a scaffold or position marker. To test this, we studied the role of the ubiquitous nuclear envelope (NE) component lamin B1 in NE stability, chr...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of cell biology 2007-02, Vol.176 (5), p.593-603
Main Authors: Malhas, Ashraf, Lee, Chiu Fan, Sanders, Rebecca, Saunders, Nigel J, Vaux, David J
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Cell adhesion & migration
Cell Compartmentation
Cell nucleus
Cells
Chromosomes
Chromosomes, Mammalian
Endopeptidases - metabolism
Gene expression
Gene Expression Profiling
Gene Expression Regulation
Genes
HeLa cells
Interphase - genetics
Interphase - physiology
Lamin Type B - genetics
Lamin Type B - metabolism
Lamins
Mice
Nuclear lamina
Nuclear Lamina - genetics
Nuclear Lamina - metabolism
Nuclear Lamina - ultrastructure
Nuclear membrane
Oligonucleotide Array Sequence Analysis
Protein Processing, Post-Translational - physiology
RNA
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Radial organization of nuclei with peripheral gene-poor chromosomes and central gene-rich chromosomes is common and could depend on the nuclear boundary as a scaffold or position marker. To test this, we studied the role of the ubiquitous nuclear envelope (NE) component lamin B1 in NE stability, chromosome territory position, and gene expression. The stability of the lamin B1 lamina is dependent on lamin endoproteolysis (by Rce1) but not carboxymethylation (by Icmt), whereas lamin C lamina stability is not affected by the loss of full-length lamin B1 or its processing. Comparison of wild-type murine fibroblasts with fibroblasts lacking full-length lamin B1, or defective in CAAX processing, identified genes that depend on a stable processed lamin B1 lamina for normal expression. We also demonstrate that the position of mouse chromosome 18 but not 19 is dependent on such a stable nuclear lamina. The results implicate processed lamin B1 in the control of gene expression as well as chromosome position.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200607054