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Identification and characterization of circulating human transitional B cells
Murine B-cell development begins in bone marrow and results in the generation of immature transitional B cells that transit to the spleen to complete their maturation. It remains unclear whether the same developmental pathway takes place in humans. Using markers characteristic of human bone marrow i...
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| Published in: | Blood 2005-06, Vol.105 (11), p.4390-4398 |
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| Main Authors: | , , , , , |
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| container_end_page | 4398 |
| container_issue | 11 |
| container_start_page | 4390 |
| container_title | Blood |
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| creator | Sims, Gary P. Ettinger, Rachel Shirota, Yuko Yarboro, Cheryl H. Illei, Gabor G. Lipsky, Peter E. |
| description | Murine B-cell development begins in bone marrow and results in the generation of immature transitional B cells that transit to the spleen to complete their maturation. It remains unclear whether the same developmental pathway takes place in humans. Using markers characteristic of human bone marrow immature B cells, we have identified a population of circulating human B cells with a phenotype most similar to mouse transitional type I (T1) B cells, although these human counterparts express CD5. These cells die rapidly in culture, and B-cell activation factor member of the tumor necrosis factor (TNF) family (BAFF) does not effect their survival regardless of B-cell receptor (BCR) stimulation. In contrast, bone marrow stromal cells or interleukin-4 (IL-4) significantly enhanced their survival. In the presence of T-cell signals provided by IL-4 or CD40 ligation, BCR stimulation can induce progression into cell cycle. Interestingly, circulating B cells that phenotypically and functionally resemble murine T2 B cells are found in cord blood and adult peripheral blood, suggesting that B-cell maturation may not be restricted to the spleen. Notably, increased proportions of T1 B cells were found in blood of patients with systemic lupus erythematosus (SLE), although bone marrow production and selection appeared to be normal. |
| doi_str_mv | 10.1182/blood-2004-11-4284 |
| format | article |
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It remains unclear whether the same developmental pathway takes place in humans. Using markers characteristic of human bone marrow immature B cells, we have identified a population of circulating human B cells with a phenotype most similar to mouse transitional type I (T1) B cells, although these human counterparts express CD5. These cells die rapidly in culture, and B-cell activation factor member of the tumor necrosis factor (TNF) family (BAFF) does not effect their survival regardless of B-cell receptor (BCR) stimulation. In contrast, bone marrow stromal cells or interleukin-4 (IL-4) significantly enhanced their survival. In the presence of T-cell signals provided by IL-4 or CD40 ligation, BCR stimulation can induce progression into cell cycle. Interestingly, circulating B cells that phenotypically and functionally resemble murine T2 B cells are found in cord blood and adult peripheral blood, suggesting that B-cell maturation may not be restricted to the spleen. Notably, increased proportions of T1 B cells were found in blood of patients with systemic lupus erythematosus (SLE), although bone marrow production and selection appeared to be normal.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood-2004-11-4284</identifier><identifier>PMID: 15701725</identifier><language>eng</language><publisher>Washington, DC: Elsevier Inc</publisher><subject>B-Cell Activating Factor ; B-Lymphocytes - cytology ; Biological and medical sciences ; Blood Cells ; Bone Marrow Cells ; Cell Cycle ; Cell Survival ; Coculture Techniques ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Immunobiology ; Immunophenotyping ; Interleukin-4 - pharmacology ; Lupus Erythematosus, Systemic - blood ; Lymphocyte Activation ; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation ; Membrane Proteins - pharmacology ; Stromal Cells ; Tumor Necrosis Factor-alpha - pharmacology</subject><ispartof>Blood, 2005-06, Vol.105 (11), p.4390-4398</ispartof><rights>2005 American Society of Hematology</rights><rights>2005 INIST-CNRS</rights><rights>2005 by The American Society of Hematology 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c549t-c645cc04cda78833c92f5f389537d4e60c835d627b21389ee8bdf8600a4227263</citedby><cites>FETCH-LOGICAL-c549t-c645cc04cda78833c92f5f389537d4e60c835d627b21389ee8bdf8600a4227263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006497120534800$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,315,786,790,891,3568,27957,27958,45815</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16826113$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15701725$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sims, Gary P.</creatorcontrib><creatorcontrib>Ettinger, Rachel</creatorcontrib><creatorcontrib>Shirota, Yuko</creatorcontrib><creatorcontrib>Yarboro, Cheryl H.</creatorcontrib><creatorcontrib>Illei, Gabor G.</creatorcontrib><creatorcontrib>Lipsky, Peter E.</creatorcontrib><title>Identification and characterization of circulating human transitional B cells</title><title>Blood</title><addtitle>Blood</addtitle><description>Murine B-cell development begins in bone marrow and results in the generation of immature transitional B cells that transit to the spleen to complete their maturation. It remains unclear whether the same developmental pathway takes place in humans. Using markers characteristic of human bone marrow immature B cells, we have identified a population of circulating human B cells with a phenotype most similar to mouse transitional type I (T1) B cells, although these human counterparts express CD5. These cells die rapidly in culture, and B-cell activation factor member of the tumor necrosis factor (TNF) family (BAFF) does not effect their survival regardless of B-cell receptor (BCR) stimulation. In contrast, bone marrow stromal cells or interleukin-4 (IL-4) significantly enhanced their survival. In the presence of T-cell signals provided by IL-4 or CD40 ligation, BCR stimulation can induce progression into cell cycle. Interestingly, circulating B cells that phenotypically and functionally resemble murine T2 B cells are found in cord blood and adult peripheral blood, suggesting that B-cell maturation may not be restricted to the spleen. Notably, increased proportions of T1 B cells were found in blood of patients with systemic lupus erythematosus (SLE), although bone marrow production and selection appeared to be normal.</description><subject>B-Cell Activating Factor</subject><subject>B-Lymphocytes - cytology</subject><subject>Biological and medical sciences</subject><subject>Blood Cells</subject><subject>Bone Marrow Cells</subject><subject>Cell Cycle</subject><subject>Cell Survival</subject><subject>Coculture Techniques</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>Immunophenotyping</subject><subject>Interleukin-4 - pharmacology</subject><subject>Lupus Erythematosus, Systemic - blood</subject><subject>Lymphocyte Activation</subject><subject>Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation</subject><subject>Membrane Proteins - pharmacology</subject><subject>Stromal Cells</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNp9kU1v1DAQhi0EotvCH-CAcoFbYPztSAipVBQqFXGBs-UdO12jrF3spBL8ehx2ReHCaTQzz3y-hDyj8IpSw15vp5x9zwBET2kvmBEPyIZKZnoABg_JBgBULwZNT8hprd8AqOBMPiYnVGqgmskN-XTlQ5rjGNHNMafOJd_hzhWHcyjx5yGYxw5jwWVqbrrpdsvepW4uLtW45t3UveswTFN9Qh6Nbqrh6dGeka-X779cfOyvP3-4uji_7lGKYe5RCYkIAr3TxnCOAxvlyM0gufYiKEDDpVdMbxlt0RDM1o9GATjBmGaKn5G3h763y3YfPLYTipvsbYl7V37Y7KL9N5Pizt7kO0vbDOCmNXh5bFDy9yXU2e5jXU9wKeSlWqWNAE2HBrIDiCXXWsL4ZwgFu6pgf6tgVxWab1cVWtHzv9e7Lzm-vQEvjoCr6KaxvRJjveeUYYpS3rg3By60Z97FUGzFGBIGH0vA2foc_7fHL1SXprg</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Sims, Gary P.</creator><creator>Ettinger, Rachel</creator><creator>Shirota, Yuko</creator><creator>Yarboro, Cheryl H.</creator><creator>Illei, Gabor G.</creator><creator>Lipsky, Peter E.</creator><general>Elsevier Inc</general><general>The Americain Society of Hematology</general><general>2005 by The American Society of Hematology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20050601</creationdate><title>Identification and characterization of circulating human transitional B cells</title><author>Sims, Gary P. ; Ettinger, Rachel ; Shirota, Yuko ; Yarboro, Cheryl H. ; Illei, Gabor G. ; Lipsky, Peter E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c549t-c645cc04cda78833c92f5f389537d4e60c835d627b21389ee8bdf8600a4227263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>B-Cell Activating Factor</topic><topic>B-Lymphocytes - cytology</topic><topic>Biological and medical sciences</topic><topic>Blood Cells</topic><topic>Bone Marrow Cells</topic><topic>Cell Cycle</topic><topic>Cell Survival</topic><topic>Coculture Techniques</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Immunobiology</topic><topic>Immunophenotyping</topic><topic>Interleukin-4 - pharmacology</topic><topic>Lupus Erythematosus, Systemic - blood</topic><topic>Lymphocyte Activation</topic><topic>Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation</topic><topic>Membrane Proteins - pharmacology</topic><topic>Stromal Cells</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sims, Gary P.</creatorcontrib><creatorcontrib>Ettinger, Rachel</creatorcontrib><creatorcontrib>Shirota, Yuko</creatorcontrib><creatorcontrib>Yarboro, Cheryl H.</creatorcontrib><creatorcontrib>Illei, Gabor G.</creatorcontrib><creatorcontrib>Lipsky, Peter E.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sims, Gary P.</au><au>Ettinger, Rachel</au><au>Shirota, Yuko</au><au>Yarboro, Cheryl H.</au><au>Illei, Gabor G.</au><au>Lipsky, Peter E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of circulating human transitional B cells</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>2005-06-01</date><risdate>2005</risdate><volume>105</volume><issue>11</issue><spage>4390</spage><epage>4398</epage><pages>4390-4398</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>Reprints: Peter E. Lipsky, Intramural Research Program Autoimmunity Branch Chief, National Institute of Arthritis and Musculoskeletal and Skin Diseases, 9000 Rockville Pike, Building 10, Room 6D47C, Bethesda, MD 20892-1860; e-mail: lipskyp@mail.nih.gov.</notes><notes>The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked “advertisement” in accordance with 18 U.S.C. section 1734.</notes><notes>Prepublished online as Blood First Edition Paper, February 8, 2005; DOI 10.1182/blood-2004-11-4284.</notes><abstract>Murine B-cell development begins in bone marrow and results in the generation of immature transitional B cells that transit to the spleen to complete their maturation. It remains unclear whether the same developmental pathway takes place in humans. Using markers characteristic of human bone marrow immature B cells, we have identified a population of circulating human B cells with a phenotype most similar to mouse transitional type I (T1) B cells, although these human counterparts express CD5. These cells die rapidly in culture, and B-cell activation factor member of the tumor necrosis factor (TNF) family (BAFF) does not effect their survival regardless of B-cell receptor (BCR) stimulation. In contrast, bone marrow stromal cells or interleukin-4 (IL-4) significantly enhanced their survival. In the presence of T-cell signals provided by IL-4 or CD40 ligation, BCR stimulation can induce progression into cell cycle. Interestingly, circulating B cells that phenotypically and functionally resemble murine T2 B cells are found in cord blood and adult peripheral blood, suggesting that B-cell maturation may not be restricted to the spleen. Notably, increased proportions of T1 B cells were found in blood of patients with systemic lupus erythematosus (SLE), although bone marrow production and selection appeared to be normal.</abstract><cop>Washington, DC</cop><pub>Elsevier Inc</pub><pmid>15701725</pmid><doi>10.1182/blood-2004-11-4284</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Blood, 2005-06, Vol.105 (11), p.4390-4398 |
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| language | eng |
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| source | ScienceDirect Journals |
| subjects | B-Cell Activating Factor B-Lymphocytes - cytology Biological and medical sciences Blood Cells Bone Marrow Cells Cell Cycle Cell Survival Coculture Techniques Flow Cytometry Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunobiology Immunophenotyping Interleukin-4 - pharmacology Lupus Erythematosus, Systemic - blood Lymphocyte Activation Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation Membrane Proteins - pharmacology Stromal Cells Tumor Necrosis Factor-alpha - pharmacology |
| title | Identification and characterization of circulating human transitional B cells |
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