Comparison of chromosomal DNA composing timeless in Drosophila melanogaster and D. virilis suggests a new conserved structure for the TIMELESS protein

Two proteins, TIM and PER, physically interact to control circadian cycles of tim and per transcription in Drosophila melanogaster. In the present study the structure of TIM protein expressed by D. virilis was determined by isolation and sequence analysis of genomic DNA (gDNA) corresponding to the D...

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Bibliographic Details
Published in:Nucleic acids research 1997-12, Vol.25 (23), p.4710-4714
Main Authors: Myers, M.P, Rothenfluh, A, Chang, M, Young, M.W
Format: Article
Language:English
Subjects:
5'-leader sequence
Amino Acid Sequence
amino acid sequences
animal proteins
Animals
aug codon
Base Sequence
binding proteins
Biological Clocks
Circadian Rhythm
cloning
Codon, Initiator
codons
complementary DNA
DNA
Drosophila
Drosophila melanogaster
Drosophila Proteins
Drosophila virilis
exons
initiation codons
Insect Proteins - chemistry
Insect Proteins - genetics
interactions
introns
loci
messenger RNA
Molecular Sequence Data
Nuclear Proteins - chemistry
Nuclear Proteins - genetics
nucleotide sequences
per protein
Period Circadian Proteins
Restriction Mapping
Sequence Alignment
species differences
strain differences
tim locus
tim protein
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Summary:Two proteins, TIM and PER, physically interact to control circadian cycles of tim and per transcription in Drosophila melanogaster. In the present study the structure of TIM protein expressed by D. virilis was determined by isolation and sequence analysis of genomic DNA (gDNA) corresponding to the D. virilis tim locus (v tim ). Comparison of v tim and m tim gDNA revealed high conservation of the TIM protein. This contrasts with poor sequence conservation previously observed for the TIM partner protein PER in these species. Inspection of the v tim sequence suggests an alternative structure for most TIM proteins. Sequences forming an intron in a previously characterized D. melanogaster tim cDNA appear to be most often translated to produce a longer TIM protein in both species. The N-terminal sequence of vTIM and sequence analysis of genomic DNA from several strains of D. melanogaster suggest that only one of two possible translation initiation sites found in tim mRNA is sufficient to generate circadian rhythms in D. melanogaster. TIM translation may be affected by multiple AUG codons that appear to have been conserved in sequences composing the 5'-untranslated tim mRNA leader.
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/25.23.4710