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Tissue- and time-dependent upregulation of cytokine mRNA in a murine model for the multiple organ dysfunction syndrome
We sought to quantitate the course of specific cytokine mRNA expression in tissues that exhibit increasing histopathological changes in time in an animal model for the multiple organ dysfunction syndrome (MODS). The development of treatment protocols for MODS requires elucidation of the mechanisms a...
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| Published in: | Annals of surgery 2004-07, Vol.240 (1), p.142-150 |
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| creator | VOLMAN, Thomas J. H GORIS, R. Jan A VAN DER MEER, Jos W. M HENDRIKS, Thijs |
| description | We sought to quantitate the course of specific cytokine mRNA expression in tissues that exhibit increasing histopathological changes in time in an animal model for the multiple organ dysfunction syndrome (MODS).
The development of treatment protocols for MODS requires elucidation of the mechanisms and mediators involved. To devise logical interventions, it is necessary to collect data on cytokine expression at tissue level during the development of MODS.
Ninety-four C57BL/6 mice were given an intraperitoneal injection of 40 microg of lipopolysaccharide (LPS), followed by zymosan at a dose of 0.8 mg/g body weight 6 days later (day 0). Six additional animals did not receive zymosan and acted as controls. At several time points after zymosan injection, 6 randomly assigned, zymosan-treated animals were killed, and their livers, lungs, spleens, and kidneys were collected. mRNA expression of tumor necrosis factor-alpha, interleukin (IL)-1beta, IL-6, macrophage migration inhibiting factor, IL-12, interferon-gamma, and IL-10 was measured using a real-time reverse transcription-polymerase chain reaction assay.
The injection of zymosan induced an acute peritonitis, followed by an apparent recovery. From approximately day 6 onwards, animals started to display MODS-like symptoms. During the peritonitis phase, up-regulation of cytokine mRNA was limited. During the period of apparent recovery, cytokine mRNA expression strongly increased, mostly reaching its maximum at day 9 when deterioration of the clinical condition had already set in. The up-regulation of tumor necrosis factor-alpha mRNA was most pronounced, especially in the lungs and liver.
Interventions should preferentially be targeted against multiple cytokines and, at least in this model, there may be a treatment window well after the initial challenge. |
| doi_str_mv | 10.1097/01.sla.0000130725.52373.e7 |
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The development of treatment protocols for MODS requires elucidation of the mechanisms and mediators involved. To devise logical interventions, it is necessary to collect data on cytokine expression at tissue level during the development of MODS.
Ninety-four C57BL/6 mice were given an intraperitoneal injection of 40 microg of lipopolysaccharide (LPS), followed by zymosan at a dose of 0.8 mg/g body weight 6 days later (day 0). Six additional animals did not receive zymosan and acted as controls. At several time points after zymosan injection, 6 randomly assigned, zymosan-treated animals were killed, and their livers, lungs, spleens, and kidneys were collected. mRNA expression of tumor necrosis factor-alpha, interleukin (IL)-1beta, IL-6, macrophage migration inhibiting factor, IL-12, interferon-gamma, and IL-10 was measured using a real-time reverse transcription-polymerase chain reaction assay.
The injection of zymosan induced an acute peritonitis, followed by an apparent recovery. From approximately day 6 onwards, animals started to display MODS-like symptoms. During the peritonitis phase, up-regulation of cytokine mRNA was limited. During the period of apparent recovery, cytokine mRNA expression strongly increased, mostly reaching its maximum at day 9 when deterioration of the clinical condition had already set in. The up-regulation of tumor necrosis factor-alpha mRNA was most pronounced, especially in the lungs and liver.
Interventions should preferentially be targeted against multiple cytokines and, at least in this model, there may be a treatment window well after the initial challenge.</description><identifier>ISSN: 0003-4932</identifier><identifier>EISSN: 1528-1140</identifier><identifier>DOI: 10.1097/01.sla.0000130725.52373.e7</identifier><identifier>PMID: 15213630</identifier><identifier>CODEN: ANSUA5</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott</publisher><subject>Animals ; Biological and medical sciences ; Cytokines - genetics ; Cytokines - metabolism ; Escherichia coli ; General aspects ; Interferon-gamma - genetics ; Interferon-gamma - metabolism ; Interleukins - genetics ; Interleukins - metabolism ; Kidney - metabolism ; Lipopolysaccharides ; Liver - metabolism ; Lung - metabolism ; Medical sciences ; Mice ; Mice, Inbred C57BL ; Multiple Organ Failure - metabolism ; Original ; Peritonitis - chemically induced ; Peritonitis - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Spleen - metabolism ; Time Factors ; Tumor Necrosis Factor-alpha - metabolism ; Up-Regulation ; Zymosan</subject><ispartof>Annals of surgery, 2004-07, Vol.240 (1), p.142-150</ispartof><rights>2004 INIST-CNRS</rights><rights>2004 Lippincott Williams & Wilkins, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-74ea2cc013579354e6bbc86b80ae8e8b95dc621e2dc99eb46ca3c29d66b99f303</citedby><cites>FETCH-LOGICAL-c452t-74ea2cc013579354e6bbc86b80ae8e8b95dc621e2dc99eb46ca3c29d66b99f303</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1356386/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1356386/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,733,786,790,891,27957,27958,53827,53829</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15913063$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15213630$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>VOLMAN, Thomas J. H</creatorcontrib><creatorcontrib>GORIS, R. Jan A</creatorcontrib><creatorcontrib>VAN DER MEER, Jos W. M</creatorcontrib><creatorcontrib>HENDRIKS, Thijs</creatorcontrib><title>Tissue- and time-dependent upregulation of cytokine mRNA in a murine model for the multiple organ dysfunction syndrome</title><title>Annals of surgery</title><addtitle>Ann Surg</addtitle><description>We sought to quantitate the course of specific cytokine mRNA expression in tissues that exhibit increasing histopathological changes in time in an animal model for the multiple organ dysfunction syndrome (MODS).
The development of treatment protocols for MODS requires elucidation of the mechanisms and mediators involved. To devise logical interventions, it is necessary to collect data on cytokine expression at tissue level during the development of MODS.
Ninety-four C57BL/6 mice were given an intraperitoneal injection of 40 microg of lipopolysaccharide (LPS), followed by zymosan at a dose of 0.8 mg/g body weight 6 days later (day 0). Six additional animals did not receive zymosan and acted as controls. At several time points after zymosan injection, 6 randomly assigned, zymosan-treated animals were killed, and their livers, lungs, spleens, and kidneys were collected. mRNA expression of tumor necrosis factor-alpha, interleukin (IL)-1beta, IL-6, macrophage migration inhibiting factor, IL-12, interferon-gamma, and IL-10 was measured using a real-time reverse transcription-polymerase chain reaction assay.
The injection of zymosan induced an acute peritonitis, followed by an apparent recovery. From approximately day 6 onwards, animals started to display MODS-like symptoms. During the peritonitis phase, up-regulation of cytokine mRNA was limited. During the period of apparent recovery, cytokine mRNA expression strongly increased, mostly reaching its maximum at day 9 when deterioration of the clinical condition had already set in. The up-regulation of tumor necrosis factor-alpha mRNA was most pronounced, especially in the lungs and liver.
Interventions should preferentially be targeted against multiple cytokines and, at least in this model, there may be a treatment window well after the initial challenge.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cytokines - genetics</subject><subject>Cytokines - metabolism</subject><subject>Escherichia coli</subject><subject>General aspects</subject><subject>Interferon-gamma - genetics</subject><subject>Interferon-gamma - metabolism</subject><subject>Interleukins - genetics</subject><subject>Interleukins - metabolism</subject><subject>Kidney - metabolism</subject><subject>Lipopolysaccharides</subject><subject>Liver - metabolism</subject><subject>Lung - metabolism</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Multiple Organ Failure - metabolism</subject><subject>Original</subject><subject>Peritonitis - chemically induced</subject><subject>Peritonitis - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Spleen - metabolism</subject><subject>Time Factors</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>Up-Regulation</subject><subject>Zymosan</subject><issn>0003-4932</issn><issn>1528-1140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNpVkd1q3DAQhUVoaTZJXyGIQi_t6seWrV4UwtI2hZBCSK6FLI13ldqSkezAvn2VzZJNdCM0-s4ZZg5CXygpKZHNN0LLNOiS5EM5aVhd1ow3vITmBK1ozdqC0op8QKsM8KKSnJ2is5QeM161pPmETjNEueBkhZ7uXUoLFFh7i2c3QmFhAm_Bz3iZImyWQc8ueBx6bHZz-Oc84PHu9go7jzUel7gvBAsD7kPE8za_lmF20wA4xI322O5Sv3izd0k7b2MY4QJ97PWQ4PPhPkcPv37er6-Lm7-__6yvbgpT1Wwumgo0MyaPWTeS1xWIrjOt6FqioYW2k7U1glFg1kgJXSWM5oZJK0QnZc8JP0c_XnynpRvBmjxW1IOaoht13KmgnXr_491WbcKTyh0Fb0U2-P5iYGJIKUL_qqVEPaehCFU5DXVMQ-3TUNBk8eXb7kfpYf0Z-HoAdDJ66KP2xqU3nMyWgvP__p-YBw</recordid><startdate>20040701</startdate><enddate>20040701</enddate><creator>VOLMAN, Thomas J. H</creator><creator>GORIS, R. Jan A</creator><creator>VAN DER MEER, Jos W. M</creator><creator>HENDRIKS, Thijs</creator><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20040701</creationdate><title>Tissue- and time-dependent upregulation of cytokine mRNA in a murine model for the multiple organ dysfunction syndrome</title><author>VOLMAN, Thomas J. H ; GORIS, R. Jan A ; VAN DER MEER, Jos W. M ; HENDRIKS, Thijs</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-74ea2cc013579354e6bbc86b80ae8e8b95dc621e2dc99eb46ca3c29d66b99f303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cytokines - genetics</topic><topic>Cytokines - metabolism</topic><topic>Escherichia coli</topic><topic>General aspects</topic><topic>Interferon-gamma - genetics</topic><topic>Interferon-gamma - metabolism</topic><topic>Interleukins - genetics</topic><topic>Interleukins - metabolism</topic><topic>Kidney - metabolism</topic><topic>Lipopolysaccharides</topic><topic>Liver - metabolism</topic><topic>Lung - metabolism</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Multiple Organ Failure - metabolism</topic><topic>Original</topic><topic>Peritonitis - chemically induced</topic><topic>Peritonitis - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Spleen - metabolism</topic><topic>Time Factors</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><topic>Up-Regulation</topic><topic>Zymosan</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>VOLMAN, Thomas J. H</creatorcontrib><creatorcontrib>GORIS, R. Jan A</creatorcontrib><creatorcontrib>VAN DER MEER, Jos W. M</creatorcontrib><creatorcontrib>HENDRIKS, Thijs</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Annals of surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>VOLMAN, Thomas J. H</au><au>GORIS, R. Jan A</au><au>VAN DER MEER, Jos W. M</au><au>HENDRIKS, Thijs</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tissue- and time-dependent upregulation of cytokine mRNA in a murine model for the multiple organ dysfunction syndrome</atitle><jtitle>Annals of surgery</jtitle><addtitle>Ann Surg</addtitle><date>2004-07-01</date><risdate>2004</risdate><volume>240</volume><issue>1</issue><spage>142</spage><epage>150</epage><pages>142-150</pages><issn>0003-4932</issn><eissn>1528-1140</eissn><coden>ANSUA5</coden><abstract>We sought to quantitate the course of specific cytokine mRNA expression in tissues that exhibit increasing histopathological changes in time in an animal model for the multiple organ dysfunction syndrome (MODS).
The development of treatment protocols for MODS requires elucidation of the mechanisms and mediators involved. To devise logical interventions, it is necessary to collect data on cytokine expression at tissue level during the development of MODS.
Ninety-four C57BL/6 mice were given an intraperitoneal injection of 40 microg of lipopolysaccharide (LPS), followed by zymosan at a dose of 0.8 mg/g body weight 6 days later (day 0). Six additional animals did not receive zymosan and acted as controls. At several time points after zymosan injection, 6 randomly assigned, zymosan-treated animals were killed, and their livers, lungs, spleens, and kidneys were collected. mRNA expression of tumor necrosis factor-alpha, interleukin (IL)-1beta, IL-6, macrophage migration inhibiting factor, IL-12, interferon-gamma, and IL-10 was measured using a real-time reverse transcription-polymerase chain reaction assay.
The injection of zymosan induced an acute peritonitis, followed by an apparent recovery. From approximately day 6 onwards, animals started to display MODS-like symptoms. During the peritonitis phase, up-regulation of cytokine mRNA was limited. During the period of apparent recovery, cytokine mRNA expression strongly increased, mostly reaching its maximum at day 9 when deterioration of the clinical condition had already set in. The up-regulation of tumor necrosis factor-alpha mRNA was most pronounced, especially in the lungs and liver.
Interventions should preferentially be targeted against multiple cytokines and, at least in this model, there may be a treatment window well after the initial challenge.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott</pub><pmid>15213630</pmid><doi>10.1097/01.sla.0000130725.52373.e7</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Annals of surgery, 2004-07, Vol.240 (1), p.142-150 |
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| source | Open Access: PubMed Central |
| subjects | Animals Biological and medical sciences Cytokines - genetics Cytokines - metabolism Escherichia coli General aspects Interferon-gamma - genetics Interferon-gamma - metabolism Interleukins - genetics Interleukins - metabolism Kidney - metabolism Lipopolysaccharides Liver - metabolism Lung - metabolism Medical sciences Mice Mice, Inbred C57BL Multiple Organ Failure - metabolism Original Peritonitis - chemically induced Peritonitis - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Spleen - metabolism Time Factors Tumor Necrosis Factor-alpha - metabolism Up-Regulation Zymosan |
| title | Tissue- and time-dependent upregulation of cytokine mRNA in a murine model for the multiple organ dysfunction syndrome |
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