Molecular analysis of recombination events in Drosophila

The locations of crossover junctions and gene conversion tracts, isolated in the rosy gene of Drosophila melanogaster, were determined using DNA sequencing and denaturing gradient gel electrophoresis. Frequent DNA sequence polymorphisms between the parental genes served as unselected genetic markers...

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Bibliographic Details
Published in:Genetics (Austin) 1989-07, Vol.122 (3), p.653-661
Main Authors: Curtis, D. (Harvard Medical School, Boston, MA), Clark, S.H, Chovnick, A, Bender, W
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Chromosome Mapping
Crossing Over, Genetic
Diptera
DNA - genetics
DROSOPHILA
Drosophila melanogaster
Drosophila melanogaster - genetics
Drosophilidae
Fundamental and applied biological sciences. Psychology
GENE
Gene Conversion
Gene Expression Regulation
GENES
Genes, Regulator
Genetic Variation
GENETICA
GENETIQUE
Genic rearrangement. Recombination. Transposable element
Investigations
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Polymorphism, Genetic
RECOMBINACION
RECOMBINAISON
Recombination, Genetic
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Summary:The locations of crossover junctions and gene conversion tracts, isolated in the rosy gene of Drosophila melanogaster, were determined using DNA sequencing and denaturing gradient gel electrophoresis. Frequent DNA sequence polymorphisms between the parental genes served as unselected genetic markers. All conversion tracts were continuous, and half of the reciprocal crossover events had conversion tracts at the crossover junction. These experiments have also identified the sequence polymorphisms responsible for altered gene expression in two naturally occurring rosy variants
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/122.3.653