Release of hepatocyte growth factor from mechanically stretched skeletal muscle satellite cells and role of pH and nitric oxide

Application of mechanical stretch to cultured adult rat muscle satellite cells results in release of hepatocyte growth factor (HGF) and accelerated entry into the cell cycle. Stretch activation of cultured rat muscle satellite cells was observed only when medium pH was between 7.1 and 7.5, even thou...

Full description

Saved in:
Bibliographic Details
Published in:Molecular biology of the cell 2002-08, Vol.13 (8), p.2909-2918
Main Authors: Tatsumi, Ryuichi, Hattori, Akihito, Ikeuchi, Yoshihide, Anderson, Judy E, Allen, Ronald E
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Culture Media, Conditioned
Culture Media, Serum-Free
Enzyme Inhibitors - metabolism
Hepatocyte Growth Factor - metabolism
Humans
Hydrogen-Ion Concentration
Male
Muscle, Skeletal - cytology
Muscle, Skeletal - metabolism
Nitric Oxide - metabolism
Nitric Oxide Synthase - antagonists & inhibitors
Nitric Oxide Synthase - metabolism
Protein Binding
Proto-Oncogene Proteins c-met - metabolism
Rats
Rats, Sprague-Dawley
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Stress, Mechanical
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Application of mechanical stretch to cultured adult rat muscle satellite cells results in release of hepatocyte growth factor (HGF) and accelerated entry into the cell cycle. Stretch activation of cultured rat muscle satellite cells was observed only when medium pH was between 7.1 and 7.5, even though activation of satellite cells was accelerated by exogenous HGF over a pH range from 6.9 to 7.8. Furthermore, HGF was only released in stretched cultures when the pH of the medium was between 7.1 and 7.4. Conditioned medium from stretched satellite cell cultures stimulated activation of unstretched satellite cells, and the addition of anti-HGF neutralizing antibodies to stretch-conditioned medium inhibited the stretch activation response. Conditioned medium from satellite cells that were stretched in the presence of nitric-oxide synthase (NOS) inhibitor N(omega)-nitro-L-arginine methyl ester hydrochloride did not accelerate activation of unstretched control satellite cells, and HGF was not released into the medium. Conditioned medium from unstretched cells that were treated with a nitric oxide donor, sodium nitroprusside dihydrate, was able to accelerate the activation of satellite cells in vitro, and HGF was found in the conditioned medium. Immunoblot analysis indicated that both neuronal and endothelial NOS isoforms were present in satellite cell cultures. Furthermore, assays of NOS activity in stretched satellite cell cultures demonstrated that NOS is stimulated when satellite cells are stretched in vitro. These experiments indicate that stretch triggers an intracellular cascade of events, including nitric oxide synthesis, which results in HGF release and satellite cell activation.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.E02-01-0062