Loading…
Cytochrome c release and mitochondria involvement in programmed cell death induced by acetic acid in Saccharomyces cerevisiae
Evidence is presented that mitochondria are implicated in the previously described programmed cell death (PCD) process induced by acetic acid in Saccharomyces cerevisiae. In yeast cells undergoing a PCD process induced by acetic acid, translocation of cytochrome c (CytC) to the cytosol and reactive...
Saved in:
| Published in: | Molecular biology of the cell 2002-08, Vol.13 (8), p.2598-2606 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c455t-5f92009aa8aae95e0bff94c6f8f59ae7ed92283e1e3a24b5bae3d7a32644c3a63 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c455t-5f92009aa8aae95e0bff94c6f8f59ae7ed92283e1e3a24b5bae3d7a32644c3a63 |
| container_end_page | 2606 |
| container_issue | 8 |
| container_start_page | 2598 |
| container_title | Molecular biology of the cell |
| container_volume | 13 |
| creator | Ludovico, Paula Rodrigues, Fernando Almeida, Agostinho Silva, Manuel T Barrientos, Antoni Côrte-Real, Manuela |
| description | Evidence is presented that mitochondria are implicated in the previously described programmed cell death (PCD) process induced by acetic acid in Saccharomyces cerevisiae. In yeast cells undergoing a PCD process induced by acetic acid, translocation of cytochrome c (CytC) to the cytosol and reactive oxygen species production, two events known to be proapoptotic in mammals, were observed. Associated with these events, reduction in oxygen consumption and in mitochondrial membrane potential was found. Enzymatic assays showed that the activity of complex bc(1) was normal, whereas that of cytochrome c oxidase (COX) was strongly decreased. This decrease is in accordance with the observed reduction in the amounts of COX II subunit and of cytochromes a+a(3). The acetic acid-induced PCD process was found to be independent of oxidative phosphorylation because it was not inhibited by oligomycin treatment. The inability of S. cerevisiae mutant strains (lacking mitochondrial DNA, heme lyase, or ATPase) to undergo acetic acid-induced PCD and in the ATPase mutant (knockout in ATP10) the absence of CytC release provides further evidence that the process is mediated by a mitochondria-dependent apoptotic pathway. The understanding of the involvement of a mitochondria-dependent apoptotic pathway in S. cerevisiae PCD process will be most useful in the further elucidation of an ancestral pathway common to PCD in metazoans. |
| doi_str_mv | 10.1091/mbc.E01-12-0161 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_117928</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72012720</sourcerecordid><originalsourceid>FETCH-LOGICAL-c455t-5f92009aa8aae95e0bff94c6f8f59ae7ed92283e1e3a24b5bae3d7a32644c3a63</originalsourceid><addsrcrecordid>eNqFUT2P1DAQtRCIu1uo6ZArutx5_JHEBQVaHRzSSRRAbU2cya1REi92dqUt7r_j6FZ8VDSekd97M_P0GHsD4hqEhZup89e3AiqQlYAanrFLsMpW2rT189ILYyswUl-wq5x_CAFa181LdgESWlBKXrLH7WmJfpfiRNzzRCNhJo5zz6ewAnHuU0Ae5mMcjzTRvJSe71N8SDhN1HNP48h7wmVXgP7gy1d34uhpCb6U0K_8r-j9DsuSk6dcJImOIQekV-zFgGOm1-e6Yd8_3n7b3lX3Xz593n64r7w2ZqnMYKUQFrFFJGtIdMNgta-HdjAWqaHeStkqAlIodWc6JNU3qGSttVdYqw17_zR3f-jK0b7YSDi6fQoTppOLGNy_yBx27iEeHUBjy-QNe3fWp_jzQHlxU8irc5wpHrJrpABZnv8SwdYN2FYW4s0T0aeYc6Lh9zEg3BqtK9E6EuBAujXaonj7t4c__HOW6heLgKQN</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19671982</pqid></control><display><type>article</type><title>Cytochrome c release and mitochondria involvement in programmed cell death induced by acetic acid in Saccharomyces cerevisiae</title><source>PubMed Central</source><creator>Ludovico, Paula ; Rodrigues, Fernando ; Almeida, Agostinho ; Silva, Manuel T ; Barrientos, Antoni ; Côrte-Real, Manuela</creator><contributor>Koshland, Douglas</contributor><creatorcontrib>Ludovico, Paula ; Rodrigues, Fernando ; Almeida, Agostinho ; Silva, Manuel T ; Barrientos, Antoni ; Côrte-Real, Manuela ; Koshland, Douglas</creatorcontrib><description>Evidence is presented that mitochondria are implicated in the previously described programmed cell death (PCD) process induced by acetic acid in Saccharomyces cerevisiae. In yeast cells undergoing a PCD process induced by acetic acid, translocation of cytochrome c (CytC) to the cytosol and reactive oxygen species production, two events known to be proapoptotic in mammals, were observed. Associated with these events, reduction in oxygen consumption and in mitochondrial membrane potential was found. Enzymatic assays showed that the activity of complex bc(1) was normal, whereas that of cytochrome c oxidase (COX) was strongly decreased. This decrease is in accordance with the observed reduction in the amounts of COX II subunit and of cytochromes a+a(3). The acetic acid-induced PCD process was found to be independent of oxidative phosphorylation because it was not inhibited by oligomycin treatment. The inability of S. cerevisiae mutant strains (lacking mitochondrial DNA, heme lyase, or ATPase) to undergo acetic acid-induced PCD and in the ATPase mutant (knockout in ATP10) the absence of CytC release provides further evidence that the process is mediated by a mitochondria-dependent apoptotic pathway. The understanding of the involvement of a mitochondria-dependent apoptotic pathway in S. cerevisiae PCD process will be most useful in the further elucidation of an ancestral pathway common to PCD in metazoans.</description><identifier>ISSN: 1059-1524</identifier><identifier>EISSN: 1939-4586</identifier><identifier>DOI: 10.1091/mbc.E01-12-0161</identifier><identifier>PMID: 12181332</identifier><language>eng</language><publisher>United States: The American Society for Cell Biology</publisher><subject>Acetic Acid - pharmacology ; Apoptosis - drug effects ; Apoptosis - physiology ; Cytochrome c Group - metabolism ; Electron Transport - physiology ; Electron Transport Complex IV - metabolism ; Membrane Potentials - physiology ; Metazoa ; Mitochondria - metabolism ; Oxidative Phosphorylation ; Reactive Oxygen Species - metabolism ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - drug effects</subject><ispartof>Molecular biology of the cell, 2002-08, Vol.13 (8), p.2598-2606</ispartof><rights>Copyright © 2002, The American Society for Cell Biology 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-5f92009aa8aae95e0bff94c6f8f59ae7ed92283e1e3a24b5bae3d7a32644c3a63</citedby><cites>FETCH-LOGICAL-c455t-5f92009aa8aae95e0bff94c6f8f59ae7ed92283e1e3a24b5bae3d7a32644c3a63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC117928/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC117928/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,733,786,790,891,27957,27958,53827,53829</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12181332$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Koshland, Douglas</contributor><creatorcontrib>Ludovico, Paula</creatorcontrib><creatorcontrib>Rodrigues, Fernando</creatorcontrib><creatorcontrib>Almeida, Agostinho</creatorcontrib><creatorcontrib>Silva, Manuel T</creatorcontrib><creatorcontrib>Barrientos, Antoni</creatorcontrib><creatorcontrib>Côrte-Real, Manuela</creatorcontrib><title>Cytochrome c release and mitochondria involvement in programmed cell death induced by acetic acid in Saccharomyces cerevisiae</title><title>Molecular biology of the cell</title><addtitle>Mol Biol Cell</addtitle><description>Evidence is presented that mitochondria are implicated in the previously described programmed cell death (PCD) process induced by acetic acid in Saccharomyces cerevisiae. In yeast cells undergoing a PCD process induced by acetic acid, translocation of cytochrome c (CytC) to the cytosol and reactive oxygen species production, two events known to be proapoptotic in mammals, were observed. Associated with these events, reduction in oxygen consumption and in mitochondrial membrane potential was found. Enzymatic assays showed that the activity of complex bc(1) was normal, whereas that of cytochrome c oxidase (COX) was strongly decreased. This decrease is in accordance with the observed reduction in the amounts of COX II subunit and of cytochromes a+a(3). The acetic acid-induced PCD process was found to be independent of oxidative phosphorylation because it was not inhibited by oligomycin treatment. The inability of S. cerevisiae mutant strains (lacking mitochondrial DNA, heme lyase, or ATPase) to undergo acetic acid-induced PCD and in the ATPase mutant (knockout in ATP10) the absence of CytC release provides further evidence that the process is mediated by a mitochondria-dependent apoptotic pathway. The understanding of the involvement of a mitochondria-dependent apoptotic pathway in S. cerevisiae PCD process will be most useful in the further elucidation of an ancestral pathway common to PCD in metazoans.</description><subject>Acetic Acid - pharmacology</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - physiology</subject><subject>Cytochrome c Group - metabolism</subject><subject>Electron Transport - physiology</subject><subject>Electron Transport Complex IV - metabolism</subject><subject>Membrane Potentials - physiology</subject><subject>Metazoa</subject><subject>Mitochondria - metabolism</subject><subject>Oxidative Phosphorylation</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - drug effects</subject><issn>1059-1524</issn><issn>1939-4586</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqFUT2P1DAQtRCIu1uo6ZArutx5_JHEBQVaHRzSSRRAbU2cya1REi92dqUt7r_j6FZ8VDSekd97M_P0GHsD4hqEhZup89e3AiqQlYAanrFLsMpW2rT189ILYyswUl-wq5x_CAFa181LdgESWlBKXrLH7WmJfpfiRNzzRCNhJo5zz6ewAnHuU0Ae5mMcjzTRvJSe71N8SDhN1HNP48h7wmVXgP7gy1d34uhpCb6U0K_8r-j9DsuSk6dcJImOIQekV-zFgGOm1-e6Yd8_3n7b3lX3Xz593n64r7w2ZqnMYKUQFrFFJGtIdMNgta-HdjAWqaHeStkqAlIodWc6JNU3qGSttVdYqw17_zR3f-jK0b7YSDi6fQoTppOLGNy_yBx27iEeHUBjy-QNe3fWp_jzQHlxU8irc5wpHrJrpABZnv8SwdYN2FYW4s0T0aeYc6Lh9zEg3BqtK9E6EuBAujXaonj7t4c__HOW6heLgKQN</recordid><startdate>200208</startdate><enddate>200208</enddate><creator>Ludovico, Paula</creator><creator>Rodrigues, Fernando</creator><creator>Almeida, Agostinho</creator><creator>Silva, Manuel T</creator><creator>Barrientos, Antoni</creator><creator>Côrte-Real, Manuela</creator><general>The American Society for Cell Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200208</creationdate><title>Cytochrome c release and mitochondria involvement in programmed cell death induced by acetic acid in Saccharomyces cerevisiae</title><author>Ludovico, Paula ; Rodrigues, Fernando ; Almeida, Agostinho ; Silva, Manuel T ; Barrientos, Antoni ; Côrte-Real, Manuela</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-5f92009aa8aae95e0bff94c6f8f59ae7ed92283e1e3a24b5bae3d7a32644c3a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Acetic Acid - pharmacology</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis - physiology</topic><topic>Cytochrome c Group - metabolism</topic><topic>Electron Transport - physiology</topic><topic>Electron Transport Complex IV - metabolism</topic><topic>Membrane Potentials - physiology</topic><topic>Metazoa</topic><topic>Mitochondria - metabolism</topic><topic>Oxidative Phosphorylation</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ludovico, Paula</creatorcontrib><creatorcontrib>Rodrigues, Fernando</creatorcontrib><creatorcontrib>Almeida, Agostinho</creatorcontrib><creatorcontrib>Silva, Manuel T</creatorcontrib><creatorcontrib>Barrientos, Antoni</creatorcontrib><creatorcontrib>Côrte-Real, Manuela</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular biology of the cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ludovico, Paula</au><au>Rodrigues, Fernando</au><au>Almeida, Agostinho</au><au>Silva, Manuel T</au><au>Barrientos, Antoni</au><au>Côrte-Real, Manuela</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytochrome c release and mitochondria involvement in programmed cell death induced by acetic acid in Saccharomyces cerevisiae</atitle><jtitle>Molecular biology of the cell</jtitle><addtitle>Mol Biol Cell</addtitle><date>2002-08</date><risdate>2002</risdate><volume>13</volume><issue>8</issue><spage>2598</spage><epage>2606</epage><pages>2598-2606</pages><issn>1059-1524</issn><eissn>1939-4586</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>Corresponding author. E-mail address: mcortereal@bio.uminho.pt.</notes><abstract>Evidence is presented that mitochondria are implicated in the previously described programmed cell death (PCD) process induced by acetic acid in Saccharomyces cerevisiae. In yeast cells undergoing a PCD process induced by acetic acid, translocation of cytochrome c (CytC) to the cytosol and reactive oxygen species production, two events known to be proapoptotic in mammals, were observed. Associated with these events, reduction in oxygen consumption and in mitochondrial membrane potential was found. Enzymatic assays showed that the activity of complex bc(1) was normal, whereas that of cytochrome c oxidase (COX) was strongly decreased. This decrease is in accordance with the observed reduction in the amounts of COX II subunit and of cytochromes a+a(3). The acetic acid-induced PCD process was found to be independent of oxidative phosphorylation because it was not inhibited by oligomycin treatment. The inability of S. cerevisiae mutant strains (lacking mitochondrial DNA, heme lyase, or ATPase) to undergo acetic acid-induced PCD and in the ATPase mutant (knockout in ATP10) the absence of CytC release provides further evidence that the process is mediated by a mitochondria-dependent apoptotic pathway. The understanding of the involvement of a mitochondria-dependent apoptotic pathway in S. cerevisiae PCD process will be most useful in the further elucidation of an ancestral pathway common to PCD in metazoans.</abstract><cop>United States</cop><pub>The American Society for Cell Biology</pub><pmid>12181332</pmid><doi>10.1091/mbc.E01-12-0161</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1059-1524 |
| ispartof | Molecular biology of the cell, 2002-08, Vol.13 (8), p.2598-2606 |
| issn | 1059-1524 1939-4586 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_117928 |
| source | PubMed Central |
| subjects | Acetic Acid - pharmacology Apoptosis - drug effects Apoptosis - physiology Cytochrome c Group - metabolism Electron Transport - physiology Electron Transport Complex IV - metabolism Membrane Potentials - physiology Metazoa Mitochondria - metabolism Oxidative Phosphorylation Reactive Oxygen Species - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - drug effects |
| title | Cytochrome c release and mitochondria involvement in programmed cell death induced by acetic acid in Saccharomyces cerevisiae |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-21T15%3A38%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cytochrome%20c%20release%20and%20mitochondria%20involvement%20in%20programmed%20cell%20death%20induced%20by%20acetic%20acid%20in%20Saccharomyces%20cerevisiae&rft.jtitle=Molecular%20biology%20of%20the%20cell&rft.au=Ludovico,%20Paula&rft.date=2002-08&rft.volume=13&rft.issue=8&rft.spage=2598&rft.epage=2606&rft.pages=2598-2606&rft.issn=1059-1524&rft.eissn=1939-4586&rft_id=info:doi/10.1091/mbc.E01-12-0161&rft_dat=%3Cproquest_pubme%3E72012720%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c455t-5f92009aa8aae95e0bff94c6f8f59ae7ed92283e1e3a24b5bae3d7a32644c3a63%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=19671982&rft_id=info:pmid/12181332&rfr_iscdi=true |