Loading…
Metabolic dormancy in Chlamydia trachomatis treated with different antibiotics
Diseases caused by spp. are often associated with persistent infections. Chlamydial persistence is commonly associated with a unique non-infectious intracellular developmental form, termed an aberrant form. Although infectious chlamydiae can be cultured consistently in cells stressed to aberrancy, t...
Saved in:
| Published in: | Infection and immunity 2024-02, Vol.92 (2), p.e0033923 |
|---|---|
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-a419t-c7a37b99cceb4fa25bddb3fb58c92ba09685dd58005c32e3553ecae27b07512a3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-a419t-c7a37b99cceb4fa25bddb3fb58c92ba09685dd58005c32e3553ecae27b07512a3 |
| container_end_page | |
| container_issue | 2 |
| container_start_page | e0033923 |
| container_title | Infection and immunity |
| container_volume | 92 |
| creator | Rockey, Daniel D Wang, Xisheng Debrine, Abigail Grieshaber, Nicole Grieshaber, Scott S |
| description | Diseases caused by
spp. are often associated with persistent infections. Chlamydial persistence is commonly associated with a unique non-infectious intracellular developmental form, termed an aberrant form. Although infectious chlamydiae can be cultured consistently in cells stressed to aberrancy, their role in persistence is not clear. Recovery from antibiotic stress was explored as a model to determine how survival of non-aberrant chlamydiae, in the presence of fully inhibitory drug concentrations, may participate in persistence. Assays included incubation in quinolones, tetracyclines, or chloramphenicol for differing lengths of time, followed by an extended recovery period in antibiotic-free media. Culturable elementary bodies were not detected during treatment with each antibiotic, but viable and culturable
emerged after the drug was removed. Time-lapse imaging of live, antibiotic-treated infected cells identified metabolically dormant developmental forms within cells that emerged to form typical productive inclusions. The effects of the increasing concentration of most tested antibiotics led to predictable inhibitory activity, in which the survival rate decreased with increasing drug concentration. In contrast, in fluoroquinolone-treated cells, there was a paradoxical increase in productive development that was directly correlated with drug concentration and inversely associated with aberrant form production. This model system uncovers a unique chlamydial persistence pathway that does not involve the chlamydial aberrant form. The association between productive latency and metabolic dormancy is consistent with models for many bacterial species and may lead to a different interpretation of mechanisms of chlamydial persistence in patients.IMPORTANCEThe life history of most pathogens within the genus
a relies on lengthy persistence in the host. The most generally accepted model for
spp. persistence involves an unusual developmental stage, termed the aberrant form, which arises during conditions that mimic a stressful host environment. In this work, we provide an alternate model for chlamydial persistence in the face of antibiotic stress. This model may be relevant to antibiotic treatment failures in patients infected with
. |
| doi_str_mv | 10.1128/iai.00339-23 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10863404</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2916409969</sourcerecordid><originalsourceid>FETCH-LOGICAL-a419t-c7a37b99cceb4fa25bddb3fb58c92ba09685dd58005c32e3553ecae27b07512a3</originalsourceid><addsrcrecordid>eNp1kctPGzEQxq0KVNLAredqjyB1Uz937VOFIlqQgF7gbI0f2xjtrlPbAeW_Z9sEVA6cZkbz0zePD6HPBC8IofJbgLDAmDFVU_YBzQhWshaC0gM0w5ioWommPUKfcn6YSs65_IiOmKSECyxn6PbGFzCxD7ZyMQ0w2m0Vxmq56mHYugBVSWBXcYAS8pR7KN5VT6GsKhe6zic_lgrGEkyIJdh8jA476LM_2cc5uv9xcbe8rK9__bxanl_XwIkqtW2BtUYpa73hHVBhnDOsM0JaRQ1g1UjhnJAYC8uoZ0Iwb8HT1uBWEApsjr7vdNcbM3hnpzUS9HqdwgBpqyME_bYzhpX-HR81wbJhHPNJ4XSvkOKfjc9FDyFb3_cw-rjJmirScKxUoyb06w61KeacfPc6h2D91wM9eaD_eaApm_CzHQ55oPohbtI4veI99sv_d7wKvxjEngEVC5G5</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2916409969</pqid></control><display><type>article</type><title>Metabolic dormancy in Chlamydia trachomatis treated with different antibiotics</title><source>American Society for Microbiology Journals</source><source>PubMed Central</source><creator>Rockey, Daniel D ; Wang, Xisheng ; Debrine, Abigail ; Grieshaber, Nicole ; Grieshaber, Scott S</creator><contributor>Monack, Denise M.</contributor><creatorcontrib>Rockey, Daniel D ; Wang, Xisheng ; Debrine, Abigail ; Grieshaber, Nicole ; Grieshaber, Scott S ; Monack, Denise M.</creatorcontrib><description>Diseases caused by
spp. are often associated with persistent infections. Chlamydial persistence is commonly associated with a unique non-infectious intracellular developmental form, termed an aberrant form. Although infectious chlamydiae can be cultured consistently in cells stressed to aberrancy, their role in persistence is not clear. Recovery from antibiotic stress was explored as a model to determine how survival of non-aberrant chlamydiae, in the presence of fully inhibitory drug concentrations, may participate in persistence. Assays included incubation in quinolones, tetracyclines, or chloramphenicol for differing lengths of time, followed by an extended recovery period in antibiotic-free media. Culturable elementary bodies were not detected during treatment with each antibiotic, but viable and culturable
emerged after the drug was removed. Time-lapse imaging of live, antibiotic-treated infected cells identified metabolically dormant developmental forms within cells that emerged to form typical productive inclusions. The effects of the increasing concentration of most tested antibiotics led to predictable inhibitory activity, in which the survival rate decreased with increasing drug concentration. In contrast, in fluoroquinolone-treated cells, there was a paradoxical increase in productive development that was directly correlated with drug concentration and inversely associated with aberrant form production. This model system uncovers a unique chlamydial persistence pathway that does not involve the chlamydial aberrant form. The association between productive latency and metabolic dormancy is consistent with models for many bacterial species and may lead to a different interpretation of mechanisms of chlamydial persistence in patients.IMPORTANCEThe life history of most pathogens within the genus
a relies on lengthy persistence in the host. The most generally accepted model for
spp. persistence involves an unusual developmental stage, termed the aberrant form, which arises during conditions that mimic a stressful host environment. In this work, we provide an alternate model for chlamydial persistence in the face of antibiotic stress. This model may be relevant to antibiotic treatment failures in patients infected with
.</description><identifier>ISSN: 0019-9567</identifier><identifier>ISSN: 1098-5522</identifier><identifier>EISSN: 1098-5522</identifier><identifier>DOI: 10.1128/iai.00339-23</identifier><identifier>PMID: 38214508</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Anti-Bacterial Agents - metabolism ; Anti-Bacterial Agents - pharmacology ; Bacterial Infections ; Bacteriology ; Chlamydia Infections - drug therapy ; Chlamydia Infections - microbiology ; Chlamydia trachomatis ; Humans</subject><ispartof>Infection and immunity, 2024-02, Vol.92 (2), p.e0033923</ispartof><rights>Copyright © 2024 American Society for Microbiology.</rights><rights>Copyright © 2024 American Society for Microbiology. 2024 American Society for Microbiology.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a419t-c7a37b99cceb4fa25bddb3fb58c92ba09685dd58005c32e3553ecae27b07512a3</citedby><cites>FETCH-LOGICAL-a419t-c7a37b99cceb4fa25bddb3fb58c92ba09685dd58005c32e3553ecae27b07512a3</cites><orcidid>0000-0003-0663-9916 ; 0000-0002-6570-8291</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.asm.org/doi/pdf/10.1128/iai.00339-23$$EPDF$$P50$$Gasm2$$H</linktopdf><linktohtml>$$Uhttps://journals.asm.org/doi/full/10.1128/iai.00339-23$$EHTML$$P50$$Gasm2$$H</linktohtml><link.rule.ids>230,315,733,786,790,891,3207,27957,27958,52786,52787,52788,53827,53829</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38214508$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Monack, Denise M.</contributor><creatorcontrib>Rockey, Daniel D</creatorcontrib><creatorcontrib>Wang, Xisheng</creatorcontrib><creatorcontrib>Debrine, Abigail</creatorcontrib><creatorcontrib>Grieshaber, Nicole</creatorcontrib><creatorcontrib>Grieshaber, Scott S</creatorcontrib><title>Metabolic dormancy in Chlamydia trachomatis treated with different antibiotics</title><title>Infection and immunity</title><addtitle>Infect Immun</addtitle><addtitle>Infect Immun</addtitle><description>Diseases caused by
spp. are often associated with persistent infections. Chlamydial persistence is commonly associated with a unique non-infectious intracellular developmental form, termed an aberrant form. Although infectious chlamydiae can be cultured consistently in cells stressed to aberrancy, their role in persistence is not clear. Recovery from antibiotic stress was explored as a model to determine how survival of non-aberrant chlamydiae, in the presence of fully inhibitory drug concentrations, may participate in persistence. Assays included incubation in quinolones, tetracyclines, or chloramphenicol for differing lengths of time, followed by an extended recovery period in antibiotic-free media. Culturable elementary bodies were not detected during treatment with each antibiotic, but viable and culturable
emerged after the drug was removed. Time-lapse imaging of live, antibiotic-treated infected cells identified metabolically dormant developmental forms within cells that emerged to form typical productive inclusions. The effects of the increasing concentration of most tested antibiotics led to predictable inhibitory activity, in which the survival rate decreased with increasing drug concentration. In contrast, in fluoroquinolone-treated cells, there was a paradoxical increase in productive development that was directly correlated with drug concentration and inversely associated with aberrant form production. This model system uncovers a unique chlamydial persistence pathway that does not involve the chlamydial aberrant form. The association between productive latency and metabolic dormancy is consistent with models for many bacterial species and may lead to a different interpretation of mechanisms of chlamydial persistence in patients.IMPORTANCEThe life history of most pathogens within the genus
a relies on lengthy persistence in the host. The most generally accepted model for
spp. persistence involves an unusual developmental stage, termed the aberrant form, which arises during conditions that mimic a stressful host environment. In this work, we provide an alternate model for chlamydial persistence in the face of antibiotic stress. This model may be relevant to antibiotic treatment failures in patients infected with
.</description><subject>Anti-Bacterial Agents - metabolism</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacterial Infections</subject><subject>Bacteriology</subject><subject>Chlamydia Infections - drug therapy</subject><subject>Chlamydia Infections - microbiology</subject><subject>Chlamydia trachomatis</subject><subject>Humans</subject><issn>0019-9567</issn><issn>1098-5522</issn><issn>1098-5522</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp1kctPGzEQxq0KVNLAredqjyB1Uz937VOFIlqQgF7gbI0f2xjtrlPbAeW_Z9sEVA6cZkbz0zePD6HPBC8IofJbgLDAmDFVU_YBzQhWshaC0gM0w5ioWommPUKfcn6YSs65_IiOmKSECyxn6PbGFzCxD7ZyMQ0w2m0Vxmq56mHYugBVSWBXcYAS8pR7KN5VT6GsKhe6zic_lgrGEkyIJdh8jA476LM_2cc5uv9xcbe8rK9__bxanl_XwIkqtW2BtUYpa73hHVBhnDOsM0JaRQ1g1UjhnJAYC8uoZ0Iwb8HT1uBWEApsjr7vdNcbM3hnpzUS9HqdwgBpqyME_bYzhpX-HR81wbJhHPNJ4XSvkOKfjc9FDyFb3_cw-rjJmirScKxUoyb06w61KeacfPc6h2D91wM9eaD_eaApm_CzHQ55oPohbtI4veI99sv_d7wKvxjEngEVC5G5</recordid><startdate>20240213</startdate><enddate>20240213</enddate><creator>Rockey, Daniel D</creator><creator>Wang, Xisheng</creator><creator>Debrine, Abigail</creator><creator>Grieshaber, Nicole</creator><creator>Grieshaber, Scott S</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0663-9916</orcidid><orcidid>https://orcid.org/0000-0002-6570-8291</orcidid></search><sort><creationdate>20240213</creationdate><title>Metabolic dormancy in Chlamydia trachomatis treated with different antibiotics</title><author>Rockey, Daniel D ; Wang, Xisheng ; Debrine, Abigail ; Grieshaber, Nicole ; Grieshaber, Scott S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a419t-c7a37b99cceb4fa25bddb3fb58c92ba09685dd58005c32e3553ecae27b07512a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Anti-Bacterial Agents - metabolism</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacterial Infections</topic><topic>Bacteriology</topic><topic>Chlamydia Infections - drug therapy</topic><topic>Chlamydia Infections - microbiology</topic><topic>Chlamydia trachomatis</topic><topic>Humans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rockey, Daniel D</creatorcontrib><creatorcontrib>Wang, Xisheng</creatorcontrib><creatorcontrib>Debrine, Abigail</creatorcontrib><creatorcontrib>Grieshaber, Nicole</creatorcontrib><creatorcontrib>Grieshaber, Scott S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Infection and immunity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rockey, Daniel D</au><au>Wang, Xisheng</au><au>Debrine, Abigail</au><au>Grieshaber, Nicole</au><au>Grieshaber, Scott S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolic dormancy in Chlamydia trachomatis treated with different antibiotics</atitle><jtitle>Infection and immunity</jtitle><stitle>Infect Immun</stitle><addtitle>Infect Immun</addtitle><date>2024-02-13</date><risdate>2024</risdate><volume>92</volume><issue>2</issue><spage>e0033923</spage><pages>e0033923-</pages><issn>0019-9567</issn><issn>1098-5522</issn><eissn>1098-5522</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>The authors declare no conflict of interest.</notes><abstract>Diseases caused by
spp. are often associated with persistent infections. Chlamydial persistence is commonly associated with a unique non-infectious intracellular developmental form, termed an aberrant form. Although infectious chlamydiae can be cultured consistently in cells stressed to aberrancy, their role in persistence is not clear. Recovery from antibiotic stress was explored as a model to determine how survival of non-aberrant chlamydiae, in the presence of fully inhibitory drug concentrations, may participate in persistence. Assays included incubation in quinolones, tetracyclines, or chloramphenicol for differing lengths of time, followed by an extended recovery period in antibiotic-free media. Culturable elementary bodies were not detected during treatment with each antibiotic, but viable and culturable
emerged after the drug was removed. Time-lapse imaging of live, antibiotic-treated infected cells identified metabolically dormant developmental forms within cells that emerged to form typical productive inclusions. The effects of the increasing concentration of most tested antibiotics led to predictable inhibitory activity, in which the survival rate decreased with increasing drug concentration. In contrast, in fluoroquinolone-treated cells, there was a paradoxical increase in productive development that was directly correlated with drug concentration and inversely associated with aberrant form production. This model system uncovers a unique chlamydial persistence pathway that does not involve the chlamydial aberrant form. The association between productive latency and metabolic dormancy is consistent with models for many bacterial species and may lead to a different interpretation of mechanisms of chlamydial persistence in patients.IMPORTANCEThe life history of most pathogens within the genus
a relies on lengthy persistence in the host. The most generally accepted model for
spp. persistence involves an unusual developmental stage, termed the aberrant form, which arises during conditions that mimic a stressful host environment. In this work, we provide an alternate model for chlamydial persistence in the face of antibiotic stress. This model may be relevant to antibiotic treatment failures in patients infected with
.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>38214508</pmid><doi>10.1128/iai.00339-23</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0003-0663-9916</orcidid><orcidid>https://orcid.org/0000-0002-6570-8291</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0019-9567 |
| ispartof | Infection and immunity, 2024-02, Vol.92 (2), p.e0033923 |
| issn | 0019-9567 1098-5522 1098-5522 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10863404 |
| source | American Society for Microbiology Journals; PubMed Central |
| subjects | Anti-Bacterial Agents - metabolism Anti-Bacterial Agents - pharmacology Bacterial Infections Bacteriology Chlamydia Infections - drug therapy Chlamydia Infections - microbiology Chlamydia trachomatis Humans |
| title | Metabolic dormancy in Chlamydia trachomatis treated with different antibiotics |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-21T07%3A32%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Metabolic%20dormancy%20in%20Chlamydia%20trachomatis%20treated%20with%20different%20antibiotics&rft.jtitle=Infection%20and%20immunity&rft.au=Rockey,%20Daniel%20D&rft.date=2024-02-13&rft.volume=92&rft.issue=2&rft.spage=e0033923&rft.pages=e0033923-&rft.issn=0019-9567&rft.eissn=1098-5522&rft_id=info:doi/10.1128/iai.00339-23&rft_dat=%3Cproquest_pubme%3E2916409969%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-a419t-c7a37b99cceb4fa25bddb3fb58c92ba09685dd58005c32e3553ecae27b07512a3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2916409969&rft_id=info:pmid/38214508&rfr_iscdi=true |