Capillary photoionization: interface for low flow rate liquid chromatography-mass spectrometry

This is the first report on capillary photoionization (CPI) interfacing a liquid chromatograph (LC) and mass spectrometer (MS). A new heated CPI ion source was developed, including a heated transfer capillary, a wide oval-shaped and low-depth ionization chamber with a vacuum ultraviolet (VUV) transp...

Full description

Saved in:
Bibliographic Details
Published in:Analyst (London) 2019-05, Vol.144 (9), p.2867-2871
Main Authors: Pöhö, Päivi, Vaikkinen, Anu, Haapala, Markus, Kylli, Petri, Kostiainen, Risto
Format: Article
Language:English
Subjects:
Feasibility studies
Flow velocity
Ion sources
Ionization chambers
Ions
Light transmittance
Linearity
Liquid chromatography
Magnesium fluorides
Mass spectrometry
Parameter sensitivity
Photoionization
Quantitative analysis
Sensitivity analysis
Steroids
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This is the first report on capillary photoionization (CPI) interfacing a liquid chromatograph (LC) and mass spectrometer (MS). A new heated CPI ion source was developed, including a heated transfer capillary, a wide oval-shaped and low-depth ionization chamber with a vacuum ultraviolet (VUV) transparent magnesium fluoride (MgF 2 ) window to increase the photoionization efficiency and thus the sensitivity. As both analytes and eluent are first vaporized and then photoionized inside the CPI ion source between the atmosphere and the vacuum of MS, the ion transfer efficiency into the MS and thus the sensitivity is improved. The effect of the most important operation parameters, the eluent flow rate and temperature of the CPI source, on the signal intensity was studied with selected steroids. The feasibility of LC-CPI-MS/MS for the quantitative analysis of steroids was also studied in terms of linearity, repeatability, and limits of detection. The method showed good quantitative performance and sensitivity down to the low femto-mole level. The first report on capillary photoionization interfacing a liquid chromatograph and mass spectrometer.
ISSN:0003-2654
1364-5528
DOI:10.1039/c9an00258h