ERK MAP KINASE IS REQUIRED IN 1,25(OH)2D3-INDUCED DIFFERENTIATION IN HUMAN OSTEOBLASTS

ABSTRACT Expression of alkaline phosphatase(ALP)activity represents a key event during the differentiation processes of osteoblasts, and the level of ALP activity has been routinely used as a relative measure of differentiation stages of osteoblasts. In human osteoblasts, we showed that vitamin D3 a...

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Published in:Immunopharmacology and immunotoxicology 2002, Vol.24 (1), p.31-41
Main Authors: Chae, H. J., Jeong, B. J., Ha, M. S., Lee, J. K., Byun, J. O., Jung, W. Y., Yun, Y. G., Lee, D. G., Oh, S. H., Chae, S. W., Kwak, Y. G., Kim, H. H., Lee, Z. H., Kim, H. R.
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - metabolism
Biological and medical sciences
Bones, joints and connective tissue. Antiinflammatory agents
Calcitriol - pharmacology
Calcium Channel Agonists - pharmacology
Cell Differentiation - drug effects
Enzyme Inhibitors - pharmacology
Flavonoids - pharmacology
Humans
Imidazoles - pharmacology
Medical sciences
Mitogen-Activated Protein Kinases - antagonists & inhibitors
Mitogen-Activated Protein Kinases - immunology
Mitogen-Activated Protein Kinases - metabolism
Osteoblasts - cytology
Osteoblasts - enzymology
Pharmacology. Drug treatments
Precipitin Tests
Pyridines - pharmacology
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Summary:ABSTRACT Expression of alkaline phosphatase(ALP)activity represents a key event during the differentiation processes of osteoblasts, and the level of ALP activity has been routinely used as a relative measure of differentiation stages of osteoblasts. In human osteoblasts, we showed that vitamin D3 analogue, 1,25(OH)2D3, had a stimulatory effect on ALP activity after 3 days, compared with control. The treatment of PD098059, an ERK MAP Kinase inhibitor, had a reducing effect on ALP activity, a differentiation marker in 1,25(OH)2D3-treated primary human osteoblasts. However, SB203580, a potent p38 MAP Kinase inhibitor, had no effect on the differentiation in this system. This indicates that ERK, not p38, is directly related to 1,25(OH)2D3 -stimulated ALP activity in primary human osteoblasts. These results also show that the vitamin D3 analogue stimulates ERK1 activation in primary human osteoblasts. This finding provides one of signaling pathways for differentiation in primary human osteoblasts.
ISSN:0892-3973
1532-2513
DOI:10.1081/IPH-120003401