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Chitooligosaccharide ameliorates diet-induced obesity in mice and affects adipose gene expression involved in adipogenesis and inflammation
Abstract Chitooligosaccharide (CO) has been reported to have potential antiobestic effects in a few studies, but the antiobesity properties of CO and its related mechanisms in models of dietary obesity remain unclear. We investigated the effect of CO on body weight gain, size of adipocytes, adipokin...
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| Published in: | Nutrition research (New York, N.Y.) N.Y.), 2012-03, Vol.32 (3), p.218-228 |
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| description | Abstract Chitooligosaccharide (CO) has been reported to have potential antiobestic effects in a few studies, but the antiobesity properties of CO and its related mechanisms in models of dietary obesity remain unclear. We investigated the effect of CO on body weight gain, size of adipocytes, adipokines, and lipid profiles in high-fat (HF) diet-induced obese mice and on the gene expression in adipose tissue using a complementary DNA microarray approach to test the hypothesis that CO supplementation would alleviate HF diet-induced obesity by the alteration of adipose tissue-specific gene expression. Male C57BL/6N mice were fed a normal diet (control), HF diet, or CO-supplemented HF diet (1% or 3%) for 5 months. Compared with the HF diet mice, mice fed the 3% CO-supplemented diet gained 15% less weight but did not display any change in food and energy intake. Chitooligosaccharide supplementation markedly improved serum and hepatic lipid profiles. Histologic examination showed that epididymal adipocyte size was smaller in mice fed the HF + 3% CO. Microarray analysis showed that dietary CO supplementation modulated adipogenesis-related genes such as matrix metallopeptidases 3 , 12 , 13 , and 14 ; tissue inhibitor of metalloproteinase 1 ; and cathepsin k in the adipose tissues. Twenty-five percent of the CO-responsive genes identified are involved in immune responses including the inflammatory response and cytokine production. These results suggest that CO supplementation may help ameliorate HF diet-induced weight gain and improve serum and liver lipid profile abnormalities, which are associated, at least in part, with altered adipose tissue gene expression involved in adipogenesis and inflammation. |
| doi_str_mv | 10.1016/j.nutres.2012.02.004 |
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We investigated the effect of CO on body weight gain, size of adipocytes, adipokines, and lipid profiles in high-fat (HF) diet-induced obese mice and on the gene expression in adipose tissue using a complementary DNA microarray approach to test the hypothesis that CO supplementation would alleviate HF diet-induced obesity by the alteration of adipose tissue-specific gene expression. Male C57BL/6N mice were fed a normal diet (control), HF diet, or CO-supplemented HF diet (1% or 3%) for 5 months. Compared with the HF diet mice, mice fed the 3% CO-supplemented diet gained 15% less weight but did not display any change in food and energy intake. Chitooligosaccharide supplementation markedly improved serum and hepatic lipid profiles. Histologic examination showed that epididymal adipocyte size was smaller in mice fed the HF + 3% CO. Microarray analysis showed that dietary CO supplementation modulated adipogenesis-related genes such as matrix metallopeptidases 3 , 12 , 13 , and 14 ; tissue inhibitor of metalloproteinase 1 ; and cathepsin k in the adipose tissues. Twenty-five percent of the CO-responsive genes identified are involved in immune responses including the inflammatory response and cytokine production. These results suggest that CO supplementation may help ameliorate HF diet-induced weight gain and improve serum and liver lipid profile abnormalities, which are associated, at least in part, with altered adipose tissue gene expression involved in adipogenesis and inflammation.</description><identifier>ISSN: 0271-5317</identifier><identifier>EISSN: 1879-0739</identifier><identifier>DOI: 10.1016/j.nutres.2012.02.004</identifier><identifier>PMID: 22464809</identifier><identifier>CODEN: NTRSDC</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adipocytes - drug effects ; Adipocytes - metabolism ; Adipocytes - pathology ; Adipogenesis ; Adipogenesis - drug effects ; Adipogenesis - genetics ; Animals ; Anti-Obesity Agents - pharmacology ; Anti-Obesity Agents - therapeutic use ; Biological and medical sciences ; Chitooligosaccharide ; Chitosan - pharmacology ; Chitosan - therapeutic use ; Cytokines - genetics ; Cytokines - metabolism ; Diet, High-Fat - adverse effects ; Dietary Fats - adverse effects ; Dietary Supplements ; Energy Intake - drug effects ; Feeding. Feeding behavior ; Fundamental and applied biological sciences. Psychology ; Gastroenterology and Hepatology ; Gene expression ; Gene Expression - drug effects ; High-fat diet ; Inflammation ; Inflammation - drug therapy ; Inflammation - genetics ; Inflammation - metabolism ; Lipid Metabolism - drug effects ; Lipid Metabolism - genetics ; Lipids - blood ; Liver - metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Obese ; Microarray Analysis ; Obesity ; Obesity - drug therapy ; Obesity - genetics ; Obesity - metabolism ; Oligosaccharides - pharmacology ; Oligosaccharides - therapeutic use ; Vertebrates: anatomy and physiology, studies on body, several organs or systems ; Weight Gain - drug effects ; Weight Gain - genetics</subject><ispartof>Nutrition research (New York, N.Y.), 2012-03, Vol.32 (3), p.218-228</ispartof><rights>Elsevier Inc.</rights><rights>2012 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-589c81d6e389e90a5df4c8390a766c009ed0838d3533a5255d1e26ba44fa13663</citedby><cites>FETCH-LOGICAL-c446t-589c81d6e389e90a5df4c8390a766c009ed0838d3533a5255d1e26ba44fa13663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25784448$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22464809$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Choi, Eun Hye</creatorcontrib><creatorcontrib>Yang, Hyun Pil</creatorcontrib><creatorcontrib>Chun, Hyang Sook</creatorcontrib><title>Chitooligosaccharide ameliorates diet-induced obesity in mice and affects adipose gene expression involved in adipogenesis and inflammation</title><title>Nutrition research (New York, N.Y.)</title><addtitle>Nutr Res</addtitle><description>Abstract Chitooligosaccharide (CO) has been reported to have potential antiobestic effects in a few studies, but the antiobesity properties of CO and its related mechanisms in models of dietary obesity remain unclear. We investigated the effect of CO on body weight gain, size of adipocytes, adipokines, and lipid profiles in high-fat (HF) diet-induced obese mice and on the gene expression in adipose tissue using a complementary DNA microarray approach to test the hypothesis that CO supplementation would alleviate HF diet-induced obesity by the alteration of adipose tissue-specific gene expression. Male C57BL/6N mice were fed a normal diet (control), HF diet, or CO-supplemented HF diet (1% or 3%) for 5 months. Compared with the HF diet mice, mice fed the 3% CO-supplemented diet gained 15% less weight but did not display any change in food and energy intake. Chitooligosaccharide supplementation markedly improved serum and hepatic lipid profiles. Histologic examination showed that epididymal adipocyte size was smaller in mice fed the HF + 3% CO. Microarray analysis showed that dietary CO supplementation modulated adipogenesis-related genes such as matrix metallopeptidases 3 , 12 , 13 , and 14 ; tissue inhibitor of metalloproteinase 1 ; and cathepsin k in the adipose tissues. Twenty-five percent of the CO-responsive genes identified are involved in immune responses including the inflammatory response and cytokine production. These results suggest that CO supplementation may help ameliorate HF diet-induced weight gain and improve serum and liver lipid profile abnormalities, which are associated, at least in part, with altered adipose tissue gene expression involved in adipogenesis and inflammation.</description><subject>Adipocytes - drug effects</subject><subject>Adipocytes - metabolism</subject><subject>Adipocytes - pathology</subject><subject>Adipogenesis</subject><subject>Adipogenesis - drug effects</subject><subject>Adipogenesis - genetics</subject><subject>Animals</subject><subject>Anti-Obesity Agents - pharmacology</subject><subject>Anti-Obesity Agents - therapeutic use</subject><subject>Biological and medical sciences</subject><subject>Chitooligosaccharide</subject><subject>Chitosan - pharmacology</subject><subject>Chitosan - therapeutic use</subject><subject>Cytokines - genetics</subject><subject>Cytokines - metabolism</subject><subject>Diet, High-Fat - adverse effects</subject><subject>Dietary Fats - adverse effects</subject><subject>Dietary Supplements</subject><subject>Energy Intake - drug effects</subject><subject>Feeding. Feeding behavior</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastroenterology and Hepatology</subject><subject>Gene expression</subject><subject>Gene Expression - drug effects</subject><subject>High-fat diet</subject><subject>Inflammation</subject><subject>Inflammation - drug therapy</subject><subject>Inflammation - genetics</subject><subject>Inflammation - metabolism</subject><subject>Lipid Metabolism - drug effects</subject><subject>Lipid Metabolism - genetics</subject><subject>Lipids - blood</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Obese</subject><subject>Microarray Analysis</subject><subject>Obesity</subject><subject>Obesity - drug therapy</subject><subject>Obesity - genetics</subject><subject>Obesity - metabolism</subject><subject>Oligosaccharides - pharmacology</subject><subject>Oligosaccharides - therapeutic use</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>Weight Gain - drug effects</subject><subject>Weight Gain - genetics</subject><issn>0271-5317</issn><issn>1879-0739</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqFksGO0zAQhiMEYsvCGyCUC-KUYseO41yQULWwSCtxAM6Wa092pyR28STV9hl4aZxtAYkL0kj24ftnRv8_RfGSszVnXL3drcM8JaB1zXi9ZrmYfFSsuG67irWie1ysWN3yqhG8vSieEe0Y4y0X4mlxUddSSc26VfFzc4dTjAPeRrLO3dmEHko7woAx2Qmo9AhThcHPDnwZt0A4HUsM5Ygug8GXtu_BTVRaj_tIUN5CgBLu93k3whgye4jDIYuz6IFZAEJ6EGPoBzuOdsrk8-JJbweCF-f3svj24err5rq6-fzx0-b9TeWkVFPV6M5p7hUI3UHHbON76bTIv1Ypx1gHnmmhvWiEsE3dNJ5DrbZWyt5yoZS4LN6c-u5T_DEDTWZEcjAMNkCcyXRKaMGZlpmUJ9KlSJSgN_uEo01Hw5lZUjA7c0rBLCkYlostslfnAfN2BP9H9Nv2DLw-A5acHfpkg0P6yzWtllLqzL07cZDtOCAkQw4h5CQwZc-Nj_i_Tf5t4AYMmGd-hyPQLs4pZKsNN5QF5styMcvB8Jpled2IX9gevz4</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Choi, Eun Hye</creator><creator>Yang, Hyun Pil</creator><creator>Chun, Hyang Sook</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120301</creationdate><title>Chitooligosaccharide ameliorates diet-induced obesity in mice and affects adipose gene expression involved in adipogenesis and inflammation</title><author>Choi, Eun Hye ; Yang, Hyun Pil ; Chun, Hyang Sook</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-589c81d6e389e90a5df4c8390a766c009ed0838d3533a5255d1e26ba44fa13663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adipocytes - drug effects</topic><topic>Adipocytes - metabolism</topic><topic>Adipocytes - pathology</topic><topic>Adipogenesis</topic><topic>Adipogenesis - drug effects</topic><topic>Adipogenesis - genetics</topic><topic>Animals</topic><topic>Anti-Obesity Agents - pharmacology</topic><topic>Anti-Obesity Agents - therapeutic use</topic><topic>Biological and medical sciences</topic><topic>Chitooligosaccharide</topic><topic>Chitosan - pharmacology</topic><topic>Chitosan - therapeutic use</topic><topic>Cytokines - genetics</topic><topic>Cytokines - metabolism</topic><topic>Diet, High-Fat - adverse effects</topic><topic>Dietary Fats - adverse effects</topic><topic>Dietary Supplements</topic><topic>Energy Intake - drug effects</topic><topic>Feeding. Feeding behavior</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastroenterology and Hepatology</topic><topic>Gene expression</topic><topic>Gene Expression - drug effects</topic><topic>High-fat diet</topic><topic>Inflammation</topic><topic>Inflammation - drug therapy</topic><topic>Inflammation - genetics</topic><topic>Inflammation - metabolism</topic><topic>Lipid Metabolism - drug effects</topic><topic>Lipid Metabolism - genetics</topic><topic>Lipids - blood</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Obese</topic><topic>Microarray Analysis</topic><topic>Obesity</topic><topic>Obesity - drug therapy</topic><topic>Obesity - genetics</topic><topic>Obesity - metabolism</topic><topic>Oligosaccharides - pharmacology</topic><topic>Oligosaccharides - therapeutic use</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>Weight Gain - drug effects</topic><topic>Weight Gain - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choi, Eun Hye</creatorcontrib><creatorcontrib>Yang, Hyun Pil</creatorcontrib><creatorcontrib>Chun, Hyang Sook</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nutrition research (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choi, Eun Hye</au><au>Yang, Hyun Pil</au><au>Chun, Hyang Sook</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chitooligosaccharide ameliorates diet-induced obesity in mice and affects adipose gene expression involved in adipogenesis and inflammation</atitle><jtitle>Nutrition research (New York, N.Y.)</jtitle><addtitle>Nutr Res</addtitle><date>2012-03-01</date><risdate>2012</risdate><volume>32</volume><issue>3</issue><spage>218</spage><epage>228</epage><pages>218-228</pages><issn>0271-5317</issn><eissn>1879-0739</eissn><coden>NTRSDC</coden><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Abstract Chitooligosaccharide (CO) has been reported to have potential antiobestic effects in a few studies, but the antiobesity properties of CO and its related mechanisms in models of dietary obesity remain unclear. We investigated the effect of CO on body weight gain, size of adipocytes, adipokines, and lipid profiles in high-fat (HF) diet-induced obese mice and on the gene expression in adipose tissue using a complementary DNA microarray approach to test the hypothesis that CO supplementation would alleviate HF diet-induced obesity by the alteration of adipose tissue-specific gene expression. Male C57BL/6N mice were fed a normal diet (control), HF diet, or CO-supplemented HF diet (1% or 3%) for 5 months. Compared with the HF diet mice, mice fed the 3% CO-supplemented diet gained 15% less weight but did not display any change in food and energy intake. Chitooligosaccharide supplementation markedly improved serum and hepatic lipid profiles. Histologic examination showed that epididymal adipocyte size was smaller in mice fed the HF + 3% CO. Microarray analysis showed that dietary CO supplementation modulated adipogenesis-related genes such as matrix metallopeptidases 3 , 12 , 13 , and 14 ; tissue inhibitor of metalloproteinase 1 ; and cathepsin k in the adipose tissues. Twenty-five percent of the CO-responsive genes identified are involved in immune responses including the inflammatory response and cytokine production. These results suggest that CO supplementation may help ameliorate HF diet-induced weight gain and improve serum and liver lipid profile abnormalities, which are associated, at least in part, with altered adipose tissue gene expression involved in adipogenesis and inflammation.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>22464809</pmid><doi>10.1016/j.nutres.2012.02.004</doi><tpages>11</tpages></addata></record> |
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| subjects | Adipocytes - drug effects Adipocytes - metabolism Adipocytes - pathology Adipogenesis Adipogenesis - drug effects Adipogenesis - genetics Animals Anti-Obesity Agents - pharmacology Anti-Obesity Agents - therapeutic use Biological and medical sciences Chitooligosaccharide Chitosan - pharmacology Chitosan - therapeutic use Cytokines - genetics Cytokines - metabolism Diet, High-Fat - adverse effects Dietary Fats - adverse effects Dietary Supplements Energy Intake - drug effects Feeding. Feeding behavior Fundamental and applied biological sciences. Psychology Gastroenterology and Hepatology Gene expression Gene Expression - drug effects High-fat diet Inflammation Inflammation - drug therapy Inflammation - genetics Inflammation - metabolism Lipid Metabolism - drug effects Lipid Metabolism - genetics Lipids - blood Liver - metabolism Male Mice Mice, Inbred C57BL Mice, Obese Microarray Analysis Obesity Obesity - drug therapy Obesity - genetics Obesity - metabolism Oligosaccharides - pharmacology Oligosaccharides - therapeutic use Vertebrates: anatomy and physiology, studies on body, several organs or systems Weight Gain - drug effects Weight Gain - genetics |
| title | Chitooligosaccharide ameliorates diet-induced obesity in mice and affects adipose gene expression involved in adipogenesis and inflammation |
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