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Development of new molecular markers for the Colletotrichum genus using RetroCl1 sequences
A nonautonomous element of 624 bp, called RetroCl1 (Retroelement Colletotrichum lindemuthianum 1), was identified in the plant pathogenic fungus Colletotrichum lindemuthianum . RetroCl1 contains terminal direct repeats (223 bp) that are surrounded by CTAGT sequences. It has a short internal domain o...
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Published in: | World journal of microbiology & biotechnology 2012-03, Vol.28 (3), p.1087-1095 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | A nonautonomous element of 624 bp, called
RetroCl1
(Retroelement
Colletotrichum lindemuthianum
1), was identified in the plant pathogenic fungus
Colletotrichum lindemuthianum
.
RetroCl1
contains terminal direct repeats (223 bp) that are surrounded by CTAGT sequences. It has a short internal domain of 178 bp and shows characteristics of terminal-repeat retrotransposon in miniature (TRIM) family. We used
RetroCl1
sequence to develop molecular markers for the
Colletotrichum
genus. IRAP (Inter-Retrotransposon Amplified Polymorphism) and REMAP (Retrotransposon-Microsatellite Amplified Polymorphism) markers were used to analyze the genetic diversity of
C
.
lindemuthianum
. Fifty-four isolates belonging to different races were used. A total of 45 loci were amplified. The Nei index showed significant differences among the populations divided according to race, indicating that they are structured according to pathotype. No clear correlation between IRAP and REMAP markers with pathogenic characterization was found.
C
.
lindemuthianum
has high genetic diversity, and the analysis of molecular variance showed that 51% of variability is found among the populations of different races. The markers were also tested in different
Colletotrichum
species. In every case, multiple bands were amplified, indicating that these markers can be successfully used in different species belonging to the
Colletotrichum
genus. |
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ISSN: | 0959-3993 1573-0972 |
DOI: | 10.1007/s11274-011-0909-x |