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Production-scale purification of the recombinant major house dust mite allergen Der f 2 mutant C8/119S
A WHO position paper states that allergen immunotherapy is an effective treatment for allergic diseases, and well characterized allergens should be used in immunotherapy. The house dust mite is a major cause of allergic disease. However, the biological activity of the mite extracts currently used ca...
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| Published in: | Journal of bioscience and bioengineering 2010-11, Vol.110 (5), p.597-601 |
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| creator | Koyanagi, Satoshi Murakami, Toshio Maeda, Toshihiro Kawatsu, Kenjirou Okamura, Hiroshi Oda, Yoshiaki Miyatsu, Yoshinobu Sugawara, Keishin Mizokami, Hiroshi |
| description | A WHO position paper states that allergen immunotherapy is an effective treatment for allergic diseases, and well characterized allergens should be used in immunotherapy. The house dust mite is a major cause of allergic disease. However, the biological activity of the mite extracts currently used cannot be clearly determined, since these extracts contain various impurities. The use of recombinant allergens can avoid this problem. However, there remains a risk of contamination by other impurities, such as host cell-derived proteins (HCPs). Advanced purification techniques are thus required to remove these contaminants. C8/119S is a mutant of the major house dust mite allergen Der f 2, and is expressed and accumulated as an inclusion body in
Escherichia coli. The C8/119S was refolded and purified through three column chromatography steps. Using this method, we could obtain about 2
g of the purified C8/119S in one purification batch. This amount is equivalent to 100,000 of the maintenance doses required for immunotherapy based on the WHO position paper. The purity of the C8/119S was 99% or more. The antigenicity of HCPs in the C8/119S was examined by passive cutaneous anaphylaxis assays. When the C8/119S was administered at 40
μg/kg, no local anaphylaxis was observed. C8/119S was thus highly purified with an extremely low level of impurities, and our procedure was shown to be an effective advanced production-scale purification process for this Der f 2 mutant. In this study, we established an advanced purification processes for C8/119S, then characterized the purified C8/119S and evaluated its purity. |
| doi_str_mv | 10.1016/j.jbiosc.2010.05.010 |
| format | article |
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Escherichia coli. The C8/119S was refolded and purified through three column chromatography steps. Using this method, we could obtain about 2
g of the purified C8/119S in one purification batch. This amount is equivalent to 100,000 of the maintenance doses required for immunotherapy based on the WHO position paper. The purity of the C8/119S was 99% or more. The antigenicity of HCPs in the C8/119S was examined by passive cutaneous anaphylaxis assays. When the C8/119S was administered at 40
μg/kg, no local anaphylaxis was observed. C8/119S was thus highly purified with an extremely low level of impurities, and our procedure was shown to be an effective advanced production-scale purification process for this Der f 2 mutant. In this study, we established an advanced purification processes for C8/119S, then characterized the purified C8/119S and evaluated its purity.</description><identifier>ISSN: 1389-1723</identifier><identifier>EISSN: 1347-4421</identifier><identifier>DOI: 10.1016/j.jbiosc.2010.05.010</identifier><identifier>PMID: 20547344</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Amino Acid Substitution ; Animals ; Antigenicity ; Antigens, Dermatophagoides - genetics ; Antigens, Dermatophagoides - immunology ; Antigens, Dermatophagoides - isolation & purification ; Arthropod Proteins ; Biological and medical sciences ; Biotechnology ; C8/119S ; Chromatography, High Pressure Liquid ; Der f 2 ; Dermatophagoides ; Dermatophagoides farinae - genetics ; Dermatophagoides farinae - immunology ; Dermatophagoides pteronyssinus ; Desensitization, Immunologic ; Electrophoresis, Polyacrylamide Gel ; Escherichia coli ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Guinea Pigs ; Humans ; Immunotherapy ; Male ; Mass Spectrometry ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Mutation ; Passive Cutaneous Anaphylaxis ; PCA ; Rabbits ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Recombinant Proteins - isolation & purification</subject><ispartof>Journal of bioscience and bioengineering, 2010-11, Vol.110 (5), p.597-601</ispartof><rights>2010 The Society for Biotechnology, Japan</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c627t-6f6eae4067008ad1dae71d0993b4c755c4ded2ac4e47f78ab1878a1614e509e53</citedby><cites>FETCH-LOGICAL-c627t-6f6eae4067008ad1dae71d0993b4c755c4ded2ac4e47f78ab1878a1614e509e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23504926$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20547344$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koyanagi, Satoshi</creatorcontrib><creatorcontrib>Murakami, Toshio</creatorcontrib><creatorcontrib>Maeda, Toshihiro</creatorcontrib><creatorcontrib>Kawatsu, Kenjirou</creatorcontrib><creatorcontrib>Okamura, Hiroshi</creatorcontrib><creatorcontrib>Oda, Yoshiaki</creatorcontrib><creatorcontrib>Miyatsu, Yoshinobu</creatorcontrib><creatorcontrib>Sugawara, Keishin</creatorcontrib><creatorcontrib>Mizokami, Hiroshi</creatorcontrib><title>Production-scale purification of the recombinant major house dust mite allergen Der f 2 mutant C8/119S</title><title>Journal of bioscience and bioengineering</title><addtitle>J Biosci Bioeng</addtitle><description>A WHO position paper states that allergen immunotherapy is an effective treatment for allergic diseases, and well characterized allergens should be used in immunotherapy. The house dust mite is a major cause of allergic disease. However, the biological activity of the mite extracts currently used cannot be clearly determined, since these extracts contain various impurities. The use of recombinant allergens can avoid this problem. However, there remains a risk of contamination by other impurities, such as host cell-derived proteins (HCPs). Advanced purification techniques are thus required to remove these contaminants. C8/119S is a mutant of the major house dust mite allergen Der f 2, and is expressed and accumulated as an inclusion body in
Escherichia coli. The C8/119S was refolded and purified through three column chromatography steps. Using this method, we could obtain about 2
g of the purified C8/119S in one purification batch. This amount is equivalent to 100,000 of the maintenance doses required for immunotherapy based on the WHO position paper. The purity of the C8/119S was 99% or more. The antigenicity of HCPs in the C8/119S was examined by passive cutaneous anaphylaxis assays. When the C8/119S was administered at 40
μg/kg, no local anaphylaxis was observed. C8/119S was thus highly purified with an extremely low level of impurities, and our procedure was shown to be an effective advanced production-scale purification process for this Der f 2 mutant. In this study, we established an advanced purification processes for C8/119S, then characterized the purified C8/119S and evaluated its purity.</description><subject>Amino Acid Sequence</subject><subject>Amino Acid Substitution</subject><subject>Animals</subject><subject>Antigenicity</subject><subject>Antigens, Dermatophagoides - genetics</subject><subject>Antigens, Dermatophagoides - immunology</subject><subject>Antigens, Dermatophagoides - isolation & purification</subject><subject>Arthropod Proteins</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>C8/119S</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Der f 2</subject><subject>Dermatophagoides</subject><subject>Dermatophagoides farinae - genetics</subject><subject>Dermatophagoides farinae - immunology</subject><subject>Dermatophagoides pteronyssinus</subject><subject>Desensitization, Immunologic</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guinea Pigs</subject><subject>Humans</subject><subject>Immunotherapy</subject><subject>Male</subject><subject>Mass Spectrometry</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis, Site-Directed</subject><subject>Mutation</subject><subject>Passive Cutaneous Anaphylaxis</subject><subject>PCA</subject><subject>Rabbits</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - immunology</subject><subject>Recombinant Proteins - isolation & purification</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqNkkuLFDEUhQtRnHH0H4hmI66qJ-_HRpD2CQMK46xDKrk1k6aq0iZVgv_eFNXqznGTEw7ffcC5TfOc4B3BRF4edocupuJ3FFcLi12VB805YVy1nFPycP1r0xJF2VnzpJQDxkRhRR43ZxQLrhjn503_Naew-DmmqS3eDYCOS4599G61UOrRfAcog09jFyc3zWh0h5TRXVoKoLCUasQZkBsGyLcwoXeQUY8oGpd5pff6khBz_bR51LuhwLOTXjQ3H95_239qr758_Lx_e9V6SdXcyl6CA46lwli7QIIDRQI2hnXcKyE8DxCo8xy46pV2HdH1JZJwENiAYBfN663vMafvC5TZjrF4GAY3Qd3YGsGFMViYe0ktjdCUKfEfpFKMSabuJZWQUmqs1558I31OpWTo7THH0eWflmC7xmsPdovXrvFaLGyVWvbiNGDpRgh_in7nWYFXJ8CtafbZTT6WvxwTmBsqK_dy43qXrLvNlbm5rpMYJtpoqtZRbzYCalw_ImRbfITJQ4j1GmYbUvz3rr8APCrMTw</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>Koyanagi, Satoshi</creator><creator>Murakami, Toshio</creator><creator>Maeda, Toshihiro</creator><creator>Kawatsu, Kenjirou</creator><creator>Okamura, Hiroshi</creator><creator>Oda, Yoshiaki</creator><creator>Miyatsu, Yoshinobu</creator><creator>Sugawara, Keishin</creator><creator>Mizokami, Hiroshi</creator><general>Elsevier B.V</general><general>Osaka, Japan: Society for Bioscience and Bioengineering, Japan; Amsterdam, the Netherlands: Distributed outside Japan by Elsevier Science</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20101101</creationdate><title>Production-scale purification of the recombinant major house dust mite allergen Der f 2 mutant C8/119S</title><author>Koyanagi, Satoshi ; Murakami, Toshio ; Maeda, Toshihiro ; Kawatsu, Kenjirou ; Okamura, Hiroshi ; Oda, Yoshiaki ; Miyatsu, Yoshinobu ; Sugawara, Keishin ; Mizokami, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c627t-6f6eae4067008ad1dae71d0993b4c755c4ded2ac4e47f78ab1878a1614e509e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Amino Acid Sequence</topic><topic>Amino Acid Substitution</topic><topic>Animals</topic><topic>Antigenicity</topic><topic>Antigens, Dermatophagoides - genetics</topic><topic>Antigens, Dermatophagoides - immunology</topic><topic>Antigens, Dermatophagoides - isolation & purification</topic><topic>Arthropod Proteins</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>C8/119S</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Der f 2</topic><topic>Dermatophagoides</topic><topic>Dermatophagoides farinae - genetics</topic><topic>Dermatophagoides farinae - immunology</topic><topic>Dermatophagoides pteronyssinus</topic><topic>Desensitization, Immunologic</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guinea Pigs</topic><topic>Humans</topic><topic>Immunotherapy</topic><topic>Male</topic><topic>Mass Spectrometry</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis, Site-Directed</topic><topic>Mutation</topic><topic>Passive Cutaneous Anaphylaxis</topic><topic>PCA</topic><topic>Rabbits</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - immunology</topic><topic>Recombinant Proteins - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koyanagi, Satoshi</creatorcontrib><creatorcontrib>Murakami, Toshio</creatorcontrib><creatorcontrib>Maeda, Toshihiro</creatorcontrib><creatorcontrib>Kawatsu, Kenjirou</creatorcontrib><creatorcontrib>Okamura, Hiroshi</creatorcontrib><creatorcontrib>Oda, Yoshiaki</creatorcontrib><creatorcontrib>Miyatsu, Yoshinobu</creatorcontrib><creatorcontrib>Sugawara, Keishin</creatorcontrib><creatorcontrib>Mizokami, Hiroshi</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koyanagi, Satoshi</au><au>Murakami, Toshio</au><au>Maeda, Toshihiro</au><au>Kawatsu, Kenjirou</au><au>Okamura, Hiroshi</au><au>Oda, Yoshiaki</au><au>Miyatsu, Yoshinobu</au><au>Sugawara, Keishin</au><au>Mizokami, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production-scale purification of the recombinant major house dust mite allergen Der f 2 mutant C8/119S</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2010-11-01</date><risdate>2010</risdate><volume>110</volume><issue>5</issue><spage>597</spage><epage>601</epage><pages>597-601</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><notes>http://dx.doi.org/10.1016/j.jbiosc.2010.05.010</notes><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>ObjectType-Article-2</notes><notes>ObjectType-Feature-1</notes><abstract>A WHO position paper states that allergen immunotherapy is an effective treatment for allergic diseases, and well characterized allergens should be used in immunotherapy. The house dust mite is a major cause of allergic disease. However, the biological activity of the mite extracts currently used cannot be clearly determined, since these extracts contain various impurities. The use of recombinant allergens can avoid this problem. However, there remains a risk of contamination by other impurities, such as host cell-derived proteins (HCPs). Advanced purification techniques are thus required to remove these contaminants. C8/119S is a mutant of the major house dust mite allergen Der f 2, and is expressed and accumulated as an inclusion body in
Escherichia coli. The C8/119S was refolded and purified through three column chromatography steps. Using this method, we could obtain about 2
g of the purified C8/119S in one purification batch. This amount is equivalent to 100,000 of the maintenance doses required for immunotherapy based on the WHO position paper. The purity of the C8/119S was 99% or more. The antigenicity of HCPs in the C8/119S was examined by passive cutaneous anaphylaxis assays. When the C8/119S was administered at 40
μg/kg, no local anaphylaxis was observed. C8/119S was thus highly purified with an extremely low level of impurities, and our procedure was shown to be an effective advanced production-scale purification process for this Der f 2 mutant. In this study, we established an advanced purification processes for C8/119S, then characterized the purified C8/119S and evaluated its purity.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>20547344</pmid><doi>10.1016/j.jbiosc.2010.05.010</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of bioscience and bioengineering, 2010-11, Vol.110 (5), p.597-601 |
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| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Amino Acid Sequence Amino Acid Substitution Animals Antigenicity Antigens, Dermatophagoides - genetics Antigens, Dermatophagoides - immunology Antigens, Dermatophagoides - isolation & purification Arthropod Proteins Biological and medical sciences Biotechnology C8/119S Chromatography, High Pressure Liquid Der f 2 Dermatophagoides Dermatophagoides farinae - genetics Dermatophagoides farinae - immunology Dermatophagoides pteronyssinus Desensitization, Immunologic Electrophoresis, Polyacrylamide Gel Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Guinea Pigs Humans Immunotherapy Male Mass Spectrometry Molecular Sequence Data Mutagenesis, Site-Directed Mutation Passive Cutaneous Anaphylaxis PCA Rabbits Recombinant Proteins - genetics Recombinant Proteins - immunology Recombinant Proteins - isolation & purification |
| title | Production-scale purification of the recombinant major house dust mite allergen Der f 2 mutant C8/119S |
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