Design of a test system based on magnetic particles with immobilized monoclonal antibodies for selective Bacillus anthracis spore concentration

Magnetic immunosorbents (MIS) have been designed using antianthrax monoclonal antibodies immobilized on aluminum silicate—iron oxide matrix activated by sodium perchlorate. MIS allows us to concentrate the analyzed microorganism and to decontaminate culture from concomitant microflora. Diagnostic te...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2011-12, Vol.47 (7), p.700-706
Main Authors: Khlyntseva, A. E, Belova, E. V, Zharnikova, I. V, Tyumentseva, I. S, Kulichenko, A. N, Dyatlov, I. A, Shemyakin, I. G
Format: Article
Language:English
Subjects:
aluminum
Atoms & subatomic particles
Bacillus anthracis
Bacteria
Biochemistry
Biomedical and Life Sciences
iron oxides
Life Sciences
Magnetic fields
Medical Microbiology
Microbiology
microorganisms
Monoclonal antibodies
sodium
spores
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Summary:Magnetic immunosorbents (MIS) have been designed using antianthrax monoclonal antibodies immobilized on aluminum silicate—iron oxide matrix activated by sodium perchlorate. MIS allows us to concentrate the analyzed microorganism and to decontaminate culture from concomitant microflora. Diagnostic test systems constructed on the basis of MIS were tested on pure Bacillus anthracis cultures and in the model experiments. The results testify to the high specificity and sensitivity (102–103 spore/ml) of the designed test systems.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683811070052