Isolation of bacterial extrachromosomal DNA from human dental plaque associated with periodontal disease, using transposon-aided capture (TRACA)

Abstract The human oral cavity is host to a complex microbial community estimated to comprise >700 bacterial species, of which at least half are thought to be not yet cultivablein vitro. To investigate the plasmids present in this community, we used a transposon-aided capture system, which allowe...

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Bibliographic Details
Published in:FEMS microbiology ecology 2011-11, Vol.78 (2), p.349-354
Main Authors: Warburton, Philip J., Allan, Elaine, Hunter, Stephanie, Ward, John, Booth, Veronica, Wade, William G., Mullany, Peter
Format: Article
Language:English
Subjects:
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Bacterial Typing Techniques
Base Sequence
dental plaque
Dental Plaque - microbiology
Deoxyribonucleic acid
DNA
DNA Transposable Elements - genetics
DNA, Bacterial - analysis
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Ecology
Humans
metagenome
Microbiology
Molecular Sequence Data
oral cavity
Oral hygiene
Periodontal Diseases - genetics
Periodontal Diseases - microbiology
plasmid
Plasmids
Plasmids - genetics
Retroelements
TRACA
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Summary:Abstract The human oral cavity is host to a complex microbial community estimated to comprise >700 bacterial species, of which at least half are thought to be not yet cultivablein vitro. To investigate the plasmids present in this community, we used a transposon-aided capture system, which allowed the isolation of plasmids from human oral supra- and subgingival plaque samples. Thirty-two novel plasmids and a circular molecule that could be an integrase-generated circular intermediate were isolated.
ISSN:0168-6496
1574-6941
DOI:10.1111/j.1574-6941.2011.01166.x