Loading…
Quantification of HIV-RNA from dried blood spots using the Siemens VERSANT® HIV-1 RNA (kPCR) assay
Objectives Simplified methods for virological monitoring in resource-limited settings are increasingly needed. We evaluated the performance of the VERSANT® HIV-1 RNA (kPCR) assay for the determination of HIV-1 viral load from dried blood spots (DBS). Assay sensitivity and correlation with plasma qua...
Saved in:
| Published in: | Journal of antimicrobial chemotherapy 2011-12, Vol.66 (12), p.2823-2826 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c409t-3a6a54945ca0dd0ca0925103d942805b8aecb0dd10988713c5885bfefeba04fc3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c409t-3a6a54945ca0dd0ca0925103d942805b8aecb0dd10988713c5885bfefeba04fc3 |
| container_end_page | 2826 |
| container_issue | 12 |
| container_start_page | 2823 |
| container_title | Journal of antimicrobial chemotherapy |
| container_volume | 66 |
| creator | Pirillo, Maria Franca Recordon-Pinson, Patricia Andreotti, Mauro Mancini, Maria Grazia Amici, Roberta Giuliano, Marina |
| description | Objectives
Simplified methods for virological monitoring in resource-limited settings are increasingly needed. We evaluated the performance of the VERSANT® HIV-1 RNA (kPCR) assay for the determination of HIV-1 viral load from dried blood spots (DBS). Assay sensitivity and correlation with plasma quantification values were assessed.
Methods
A total of 98 DBS were prepared from fresh blood samples of HIV-infected patients. DBS were kept at room temperature for 6 weeks or 7 months before processing while the corresponding plasma samples were stored at −80°C. DBS were first pre-treated in a special DBS buffer. The DBS extracts and the plasma samples were then purified and amplified using the VERSANT assay reagents.
Results
In the first series of tests, performed after 6 weeks of storage, there was good correlation between quantification of viral load in plasma and in DBS (r = 0.95, P 1000 copies/mL. The sensitivity and specificity of the DBS assay were 88.2% [95% confidence interval (CI) 79.4-93.6] and 69.2% (95% CI 42.0-87.4), respectively. Using the 5000 copies/mL threshold (defining virological failure in resource-limited settings), both positive and negative predictive values were high (95.2% and 87.5%, respectively). After 7 months of storage there was a modest decrease in the detection rate and less significant correlations for samples with HIV-RNA |
| doi_str_mv | 10.1093/jac/dkr383 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_904222458</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/jac/dkr383</oup_id><sourcerecordid>2516229621</sourcerecordid><originalsourceid>FETCH-LOGICAL-c409t-3a6a54945ca0dd0ca0925103d942805b8aecb0dd10988713c5885bfefeba04fc3</originalsourceid><addsrcrecordid>eNp90Mtu1DAUBmCrAtGhsOEBkIVUcZFCj2-JvRyNCq1UlXZ62UaOY7eeJvFgJ4u-FA_Bk-EyA5W66MZenO8cH_8IvSPwlYBiByttDtq7yCTbQTPCSygoKPICzYCBKCou2C56ndIKAEpRyldolxKVSxWdIXM-6WH0zhs9-jDg4PDR8XWxPJ1jF0OP2-hti5suhBandRgTnpIfbvB4a_GFt70dEr4-XF7MTy9___rbSvBD86e7s8XyM9Yp6fs36KXTXbJvt_ceuvp2eLk4Kk5-fD9ezE8Kw0GNBdOlFlxxYTS0LeRTUUGAtYpTCaKR2pomV_KfpawIM0JK0TjrbKOBO8P20MfN3HUMPyebxrr3ydiu04MNU6oVcEopFzLLD0_kKkxxyMtlJEABCJLRlw0yMaQUravX0fc63tcE6ofg6xx8vQk-4_fbiVPT2_Y__Zd0BvtboJPRnYt6MD49Ol5VwGj56MK0fu7BP0mDlcY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>905090051</pqid></control><display><type>article</type><title>Quantification of HIV-RNA from dried blood spots using the Siemens VERSANT® HIV-1 RNA (kPCR) assay</title><source>Oxford Journals</source><creator>Pirillo, Maria Franca ; Recordon-Pinson, Patricia ; Andreotti, Mauro ; Mancini, Maria Grazia ; Amici, Roberta ; Giuliano, Marina</creator><creatorcontrib>Pirillo, Maria Franca ; Recordon-Pinson, Patricia ; Andreotti, Mauro ; Mancini, Maria Grazia ; Amici, Roberta ; Giuliano, Marina</creatorcontrib><description>Objectives
Simplified methods for virological monitoring in resource-limited settings are increasingly needed. We evaluated the performance of the VERSANT® HIV-1 RNA (kPCR) assay for the determination of HIV-1 viral load from dried blood spots (DBS). Assay sensitivity and correlation with plasma quantification values were assessed.
Methods
A total of 98 DBS were prepared from fresh blood samples of HIV-infected patients. DBS were kept at room temperature for 6 weeks or 7 months before processing while the corresponding plasma samples were stored at −80°C. DBS were first pre-treated in a special DBS buffer. The DBS extracts and the plasma samples were then purified and amplified using the VERSANT assay reagents.
Results
In the first series of tests, performed after 6 weeks of storage, there was good correlation between quantification of viral load in plasma and in DBS (r = 0.95, P < 0.001). The detection rate in DBS was 100% when plasma levels were >1000 copies/mL. The sensitivity and specificity of the DBS assay were 88.2% [95% confidence interval (CI) 79.4-93.6] and 69.2% (95% CI 42.0-87.4), respectively. Using the 5000 copies/mL threshold (defining virological failure in resource-limited settings), both positive and negative predictive values were high (95.2% and 87.5%, respectively). After 7 months of storage there was a modest decrease in the detection rate and less significant correlations for samples with HIV-RNA <5000 copies/mL.
Conclusions
Quantification of HIV-RNA from DBS by the VERSANT automated sample preparation and detection method can be used to diagnose virological failure in HIV-positive patients.</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/dkr383</identifier><identifier>PMID: 21930572</identifier><identifier>CODEN: JACHDX</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents ; Automation - methods ; Biological and medical sciences ; Blood - virology ; Correlation analysis ; Desiccation - methods ; HIV ; HIV Infections - virology ; HIV-1 - isolation & purification ; Human immunodeficiency virus ; Human viral diseases ; Humans ; Immunodeficiencies ; Immunodeficiencies. Immunoglobulinopathies ; Immunopathology ; Infectious diseases ; Medical sciences ; Molecular Diagnostic Techniques - methods ; Pharmacology. Drug treatments ; Plasma ; Reagent Kits, Diagnostic ; Ribonucleic acid ; RNA ; RNA, Viral - blood ; Sensitivity and Specificity ; Specimen Handling - methods ; Viral diseases ; Viral diseases of the lymphoid tissue and the blood. Aids ; Viral Load ; Virology ; Virology - methods</subject><ispartof>Journal of antimicrobial chemotherapy, 2011-12, Vol.66 (12), p.2823-2826</ispartof><rights>The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com 2011</rights><rights>2015 INIST-CNRS</rights><rights>Copyright Oxford Publishing Limited(England) Dec 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-3a6a54945ca0dd0ca0925103d942805b8aecb0dd10988713c5885bfefeba04fc3</citedby><cites>FETCH-LOGICAL-c409t-3a6a54945ca0dd0ca0925103d942805b8aecb0dd10988713c5885bfefeba04fc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,1591,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24770326$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21930572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pirillo, Maria Franca</creatorcontrib><creatorcontrib>Recordon-Pinson, Patricia</creatorcontrib><creatorcontrib>Andreotti, Mauro</creatorcontrib><creatorcontrib>Mancini, Maria Grazia</creatorcontrib><creatorcontrib>Amici, Roberta</creatorcontrib><creatorcontrib>Giuliano, Marina</creatorcontrib><title>Quantification of HIV-RNA from dried blood spots using the Siemens VERSANT® HIV-1 RNA (kPCR) assay</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J Antimicrob Chemother</addtitle><description>Objectives
Simplified methods for virological monitoring in resource-limited settings are increasingly needed. We evaluated the performance of the VERSANT® HIV-1 RNA (kPCR) assay for the determination of HIV-1 viral load from dried blood spots (DBS). Assay sensitivity and correlation with plasma quantification values were assessed.
Methods
A total of 98 DBS were prepared from fresh blood samples of HIV-infected patients. DBS were kept at room temperature for 6 weeks or 7 months before processing while the corresponding plasma samples were stored at −80°C. DBS were first pre-treated in a special DBS buffer. The DBS extracts and the plasma samples were then purified and amplified using the VERSANT assay reagents.
Results
In the first series of tests, performed after 6 weeks of storage, there was good correlation between quantification of viral load in plasma and in DBS (r = 0.95, P < 0.001). The detection rate in DBS was 100% when plasma levels were >1000 copies/mL. The sensitivity and specificity of the DBS assay were 88.2% [95% confidence interval (CI) 79.4-93.6] and 69.2% (95% CI 42.0-87.4), respectively. Using the 5000 copies/mL threshold (defining virological failure in resource-limited settings), both positive and negative predictive values were high (95.2% and 87.5%, respectively). After 7 months of storage there was a modest decrease in the detection rate and less significant correlations for samples with HIV-RNA <5000 copies/mL.
Conclusions
Quantification of HIV-RNA from DBS by the VERSANT automated sample preparation and detection method can be used to diagnose virological failure in HIV-positive patients.</description><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Automation - methods</subject><subject>Biological and medical sciences</subject><subject>Blood - virology</subject><subject>Correlation analysis</subject><subject>Desiccation - methods</subject><subject>HIV</subject><subject>HIV Infections - virology</subject><subject>HIV-1 - isolation & purification</subject><subject>Human immunodeficiency virus</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Immunodeficiencies</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunopathology</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Molecular Diagnostic Techniques - methods</subject><subject>Pharmacology. Drug treatments</subject><subject>Plasma</subject><subject>Reagent Kits, Diagnostic</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Viral - blood</subject><subject>Sensitivity and Specificity</subject><subject>Specimen Handling - methods</subject><subject>Viral diseases</subject><subject>Viral diseases of the lymphoid tissue and the blood. Aids</subject><subject>Viral Load</subject><subject>Virology</subject><subject>Virology - methods</subject><issn>0305-7453</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp90Mtu1DAUBmCrAtGhsOEBkIVUcZFCj2-JvRyNCq1UlXZ62UaOY7eeJvFgJ4u-FA_Bk-EyA5W66MZenO8cH_8IvSPwlYBiByttDtq7yCTbQTPCSygoKPICzYCBKCou2C56ndIKAEpRyldolxKVSxWdIXM-6WH0zhs9-jDg4PDR8XWxPJ1jF0OP2-hti5suhBandRgTnpIfbvB4a_GFt70dEr4-XF7MTy9___rbSvBD86e7s8XyM9Yp6fs36KXTXbJvt_ceuvp2eLk4Kk5-fD9ezE8Kw0GNBdOlFlxxYTS0LeRTUUGAtYpTCaKR2pomV_KfpawIM0JK0TjrbKOBO8P20MfN3HUMPyebxrr3ydiu04MNU6oVcEopFzLLD0_kKkxxyMtlJEABCJLRlw0yMaQUravX0fc63tcE6ofg6xx8vQk-4_fbiVPT2_Y__Zd0BvtboJPRnYt6MD49Ol5VwGj56MK0fu7BP0mDlcY</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Pirillo, Maria Franca</creator><creator>Recordon-Pinson, Patricia</creator><creator>Andreotti, Mauro</creator><creator>Mancini, Maria Grazia</creator><creator>Amici, Roberta</creator><creator>Giuliano, Marina</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20111201</creationdate><title>Quantification of HIV-RNA from dried blood spots using the Siemens VERSANT® HIV-1 RNA (kPCR) assay</title><author>Pirillo, Maria Franca ; Recordon-Pinson, Patricia ; Andreotti, Mauro ; Mancini, Maria Grazia ; Amici, Roberta ; Giuliano, Marina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-3a6a54945ca0dd0ca0925103d942805b8aecb0dd10988713c5885bfefeba04fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Automation - methods</topic><topic>Biological and medical sciences</topic><topic>Blood - virology</topic><topic>Correlation analysis</topic><topic>Desiccation - methods</topic><topic>HIV</topic><topic>HIV Infections - virology</topic><topic>HIV-1 - isolation & purification</topic><topic>Human immunodeficiency virus</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Immunodeficiencies</topic><topic>Immunodeficiencies. Immunoglobulinopathies</topic><topic>Immunopathology</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Molecular Diagnostic Techniques - methods</topic><topic>Pharmacology. Drug treatments</topic><topic>Plasma</topic><topic>Reagent Kits, Diagnostic</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Viral - blood</topic><topic>Sensitivity and Specificity</topic><topic>Specimen Handling - methods</topic><topic>Viral diseases</topic><topic>Viral diseases of the lymphoid tissue and the blood. Aids</topic><topic>Viral Load</topic><topic>Virology</topic><topic>Virology - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pirillo, Maria Franca</creatorcontrib><creatorcontrib>Recordon-Pinson, Patricia</creatorcontrib><creatorcontrib>Andreotti, Mauro</creatorcontrib><creatorcontrib>Mancini, Maria Grazia</creatorcontrib><creatorcontrib>Amici, Roberta</creatorcontrib><creatorcontrib>Giuliano, Marina</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pirillo, Maria Franca</au><au>Recordon-Pinson, Patricia</au><au>Andreotti, Mauro</au><au>Mancini, Maria Grazia</au><au>Amici, Roberta</au><au>Giuliano, Marina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of HIV-RNA from dried blood spots using the Siemens VERSANT® HIV-1 RNA (kPCR) assay</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J Antimicrob Chemother</addtitle><date>2011-12-01</date><risdate>2011</risdate><volume>66</volume><issue>12</issue><spage>2823</spage><epage>2826</epage><pages>2823-2826</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><coden>JACHDX</coden><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Objectives
Simplified methods for virological monitoring in resource-limited settings are increasingly needed. We evaluated the performance of the VERSANT® HIV-1 RNA (kPCR) assay for the determination of HIV-1 viral load from dried blood spots (DBS). Assay sensitivity and correlation with plasma quantification values were assessed.
Methods
A total of 98 DBS were prepared from fresh blood samples of HIV-infected patients. DBS were kept at room temperature for 6 weeks or 7 months before processing while the corresponding plasma samples were stored at −80°C. DBS were first pre-treated in a special DBS buffer. The DBS extracts and the plasma samples were then purified and amplified using the VERSANT assay reagents.
Results
In the first series of tests, performed after 6 weeks of storage, there was good correlation between quantification of viral load in plasma and in DBS (r = 0.95, P < 0.001). The detection rate in DBS was 100% when plasma levels were >1000 copies/mL. The sensitivity and specificity of the DBS assay were 88.2% [95% confidence interval (CI) 79.4-93.6] and 69.2% (95% CI 42.0-87.4), respectively. Using the 5000 copies/mL threshold (defining virological failure in resource-limited settings), both positive and negative predictive values were high (95.2% and 87.5%, respectively). After 7 months of storage there was a modest decrease in the detection rate and less significant correlations for samples with HIV-RNA <5000 copies/mL.
Conclusions
Quantification of HIV-RNA from DBS by the VERSANT automated sample preparation and detection method can be used to diagnose virological failure in HIV-positive patients.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>21930572</pmid><doi>10.1093/jac/dkr383</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-7453 |
| ispartof | Journal of antimicrobial chemotherapy, 2011-12, Vol.66 (12), p.2823-2826 |
| issn | 0305-7453 1460-2091 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_904222458 |
| source | Oxford Journals |
| subjects | Antibiotics. Antiinfectious agents. Antiparasitic agents Automation - methods Biological and medical sciences Blood - virology Correlation analysis Desiccation - methods HIV HIV Infections - virology HIV-1 - isolation & purification Human immunodeficiency virus Human viral diseases Humans Immunodeficiencies Immunodeficiencies. Immunoglobulinopathies Immunopathology Infectious diseases Medical sciences Molecular Diagnostic Techniques - methods Pharmacology. Drug treatments Plasma Reagent Kits, Diagnostic Ribonucleic acid RNA RNA, Viral - blood Sensitivity and Specificity Specimen Handling - methods Viral diseases Viral diseases of the lymphoid tissue and the blood. Aids Viral Load Virology Virology - methods |
| title | Quantification of HIV-RNA from dried blood spots using the Siemens VERSANT® HIV-1 RNA (kPCR) assay |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-22T21%3A25%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantification%20of%20HIV-RNA%20from%20dried%20blood%20spots%20using%20the%20Siemens%20VERSANT%C2%AE%20HIV-1%20RNA%20(kPCR)%20assay&rft.jtitle=Journal%20of%20antimicrobial%20chemotherapy&rft.au=Pirillo,%20Maria%20Franca&rft.date=2011-12-01&rft.volume=66&rft.issue=12&rft.spage=2823&rft.epage=2826&rft.pages=2823-2826&rft.issn=0305-7453&rft.eissn=1460-2091&rft.coden=JACHDX&rft_id=info:doi/10.1093/jac/dkr383&rft_dat=%3Cproquest_cross%3E2516229621%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c409t-3a6a54945ca0dd0ca0925103d942805b8aecb0dd10988713c5885bfefeba04fc3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=905090051&rft_id=info:pmid/21930572&rft_oup_id=10.1093/jac/dkr383&rfr_iscdi=true |