Somatic embryogenesis and organogenesis in Dendrocalamus hamiltonii

In this study, mature zygotic embryos, plant growth regulators, and various media were tested with the aim of developing an efficient regeneration system for plantlets of the bamboo species Dendrocalamus hamiltonii. Callus formation was induced in explants cultured in Murashige and Skoog (MS) medium...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2010-12, Vol.103 (3), p.325-332
Main Authors: Zhang, Ning, Fang, Wei, Shi, Yan, Liu, Qianqian, Yang, Haiyun, Gui, Renyi, Lin, Xinchun
Format: Article
Language:English
Subjects:
Bamboo
Benzyladenine
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Butyric acid
Callus
Culture media
Dendrocalamus
Dendrocalamus hamiltonii
Dichlorophenoxyacetic acid
Differentiation
Embryonic growth stage
Embryos
Explants
Fundamental and applied biological sciences. Psychology
Growth regulators
Indole-3-butyric acid
Kinetin
Life Sciences
Naphthaleneacetic acid
Organogenesis
Original Paper
Plant Genetics and Genomics
Plant growth
Plant Pathology
Plant Physiology
Plant Sciences
Plantlets
Regeneration
Somatic embryogenesis
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Summary:In this study, mature zygotic embryos, plant growth regulators, and various media were tested with the aim of developing an efficient regeneration system for plantlets of the bamboo species Dendrocalamus hamiltonii. Callus formation was induced in explants cultured in Murashige and Skoog (MS) medium supplemented with 1.0–3.0 mg/l 2,4-dichlorophenoxyacetic acid. Optimal shoot differentiation and subsequent shoot growth were also obtained in MS medium supplemented with 2 mg/l benzyladenine, 1 mg/l kinetin, and 1 mg/l naphthaleneacetic acid. Root induction was enhanced by the addition of 5 mg/l indole-3-butyric acid to the culture medium. Histological analysis revealed that both somatic embryogenesis and organogenesis were induced during callus initiation, shoot differentiation, and the development of plantlets from the mature zygotic embryos. Our data provide a useful basis for developing culture protocols for the regeneration of bamboo plants.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-010-9783-8