Kinetics of relaxation by cGMP/cGKI signaling in fundus smooth muscle

cGMP-dependent kinase I (cGKI) is a major mediator of smooth muscle relaxation and exists in two isoforms, α and β. Both isoforms are supposed to mediate their effects via different intracellular signaling pathways. To verify this concept, the kinetics of relaxation mediated by either isoform was an...

Full description

Saved in:
Bibliographic Details
Published in:European journal of pharmacology 2011-11, Vol.670 (1), p.266-271
Main Authors: Ertl, Claudia, Lukowski, Robert, Sigl, Katja, Schlossmann, Jens, Hofmann, Franz, Wegener, Jörg W.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
cGMP
Cyclic GMP - analogs & derivatives
Cyclic GMP - metabolism
Cyclic GMP - pharmacology
Electric Stimulation
Female
Fundus
Gastric Fundus
Gene Expression Regulation - drug effects
In Vitro Techniques
Intracellular Signaling Peptides and Proteins - metabolism
Kinetics
Male
Medical sciences
Mice
Muscle Relaxation - drug effects
Muscle, Smooth - cytology
Muscle, Smooth - drug effects
Muscle, Smooth - metabolism
Muscle, Smooth - physiology
NANC
Neurons - drug effects
Neurons - metabolism
Nitric oxide
Pharmacology. Drug treatments
Protein Isoforms - metabolism
Relaxation
Signal Transduction - drug effects
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c391t-a1d01cab99a3f8b99d0873de8508822826f938db080942852c56192ad15d29af3
cites cdi_FETCH-LOGICAL-c391t-a1d01cab99a3f8b99d0873de8508822826f938db080942852c56192ad15d29af3
container_end_page 271
container_issue 1
container_start_page 266
container_title European journal of pharmacology
container_volume 670
creator Ertl, Claudia
Lukowski, Robert
Sigl, Katja
Schlossmann, Jens
Hofmann, Franz
Wegener, Jörg W.
description cGMP-dependent kinase I (cGKI) is a major mediator of smooth muscle relaxation and exists in two isoforms, α and β. Both isoforms are supposed to mediate their effects via different intracellular signaling pathways. To verify this concept, the kinetics of relaxation mediated by either isoform was analyzed in gastric fundus smooth muscle from mice. Muscles from mice that express selectively the Iα or Iβ isoform of cGKI in smooth muscle (sm-cGKIα or sm-cGKIβ mice) were compared to muscles from conventional cGKI−/− mice. Fundus muscles were contracted by carbachol and then relaxed by 8-Br-cGMP or by electrical field stimulation (EFS). The time course of relaxation by 8-Br-cGMP was not different between muscles from sm-cGKIα and sm-cGKIβ mice. EFS induced a fast transient relaxation in muscles from sm-cGKIα and sm-cGKIβ mice that was blocked by the NO synthase inhibitor L-NAME. Recovery from this relaxation was about 4-times slower in muscles from sm-cGKIα mice than in muscles from sm-cGKIβ mice. The different kinetic of recovery from relaxation after EFS in sm-cGKIα and sm-cGKIβ mice suggests that different signaling pathways exist for each cGKI isoform in vivo in fundus muscles.
doi_str_mv 10.1016/j.ejphar.2011.07.048
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_898504140</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0014299911008879</els_id><sourcerecordid>898504140</sourcerecordid><originalsourceid>FETCH-LOGICAL-c391t-a1d01cab99a3f8b99d0873de8508822826f938db080942852c56192ad15d29af3</originalsourceid><addsrcrecordid>eNp9kD1PwzAQhi0EgvLxDxDygpgS7pyksRckVEFBFMEAs-XaDrhKnGIniP57UrXAxi23PO97p4eQU4QUAceXi9Qulu8qpAwQUyhTyPkOGSEvRQIlsl0yAsA8YUKIA3IY4wIACsGKfXLAUGA-zIjcPDhvO6cjbSsabK2-VOdaT-crqqePz5d6-nBPo3vzqnb-jTpPq96bPtLYtG33Tps-6toek71K1dGebPcReb29eZncJbOn6f3kepboTGCXKDSAWs2FUFnFh2WAl5mxvADOGeNsXImMmzlwEDnjBdPFGAVTBgvDhKqyI3Kx6V2G9qO3sZONi9rWtfK27aPkYqjKMYeBzDekDm2MwVZyGVyjwkoiyLU_uZAbf3LtT0IpB39D7Gx7oJ831vyGfoQNwPkWUFGrugrKaxf_uHyclRzX3NWGs4OOT2eDjNpZr61xwepOmtb9_8k3yneOPg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>898504140</pqid></control><display><type>article</type><title>Kinetics of relaxation by cGMP/cGKI signaling in fundus smooth muscle</title><source>ScienceDirect Journals</source><creator>Ertl, Claudia ; Lukowski, Robert ; Sigl, Katja ; Schlossmann, Jens ; Hofmann, Franz ; Wegener, Jörg W.</creator><creatorcontrib>Ertl, Claudia ; Lukowski, Robert ; Sigl, Katja ; Schlossmann, Jens ; Hofmann, Franz ; Wegener, Jörg W.</creatorcontrib><description>cGMP-dependent kinase I (cGKI) is a major mediator of smooth muscle relaxation and exists in two isoforms, α and β. Both isoforms are supposed to mediate their effects via different intracellular signaling pathways. To verify this concept, the kinetics of relaxation mediated by either isoform was analyzed in gastric fundus smooth muscle from mice. Muscles from mice that express selectively the Iα or Iβ isoform of cGKI in smooth muscle (sm-cGKIα or sm-cGKIβ mice) were compared to muscles from conventional cGKI−/− mice. Fundus muscles were contracted by carbachol and then relaxed by 8-Br-cGMP or by electrical field stimulation (EFS). The time course of relaxation by 8-Br-cGMP was not different between muscles from sm-cGKIα and sm-cGKIβ mice. EFS induced a fast transient relaxation in muscles from sm-cGKIα and sm-cGKIβ mice that was blocked by the NO synthase inhibitor L-NAME. Recovery from this relaxation was about 4-times slower in muscles from sm-cGKIα mice than in muscles from sm-cGKIβ mice. The different kinetic of recovery from relaxation after EFS in sm-cGKIα and sm-cGKIβ mice suggests that different signaling pathways exist for each cGKI isoform in vivo in fundus muscles.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/j.ejphar.2011.07.048</identifier><identifier>PMID: 21914444</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; cGMP ; Cyclic GMP - analogs &amp; derivatives ; Cyclic GMP - metabolism ; Cyclic GMP - pharmacology ; Electric Stimulation ; Female ; Fundus ; Gastric Fundus ; Gene Expression Regulation - drug effects ; In Vitro Techniques ; Intracellular Signaling Peptides and Proteins - metabolism ; Kinetics ; Male ; Medical sciences ; Mice ; Muscle Relaxation - drug effects ; Muscle, Smooth - cytology ; Muscle, Smooth - drug effects ; Muscle, Smooth - metabolism ; Muscle, Smooth - physiology ; NANC ; Neurons - drug effects ; Neurons - metabolism ; Nitric oxide ; Pharmacology. Drug treatments ; Protein Isoforms - metabolism ; Relaxation ; Signal Transduction - drug effects</subject><ispartof>European journal of pharmacology, 2011-11, Vol.670 (1), p.266-271</ispartof><rights>2011</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-a1d01cab99a3f8b99d0873de8508822826f938db080942852c56192ad15d29af3</citedby><cites>FETCH-LOGICAL-c391t-a1d01cab99a3f8b99d0873de8508822826f938db080942852c56192ad15d29af3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,783,787,27936,27937</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=24637814$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21914444$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ertl, Claudia</creatorcontrib><creatorcontrib>Lukowski, Robert</creatorcontrib><creatorcontrib>Sigl, Katja</creatorcontrib><creatorcontrib>Schlossmann, Jens</creatorcontrib><creatorcontrib>Hofmann, Franz</creatorcontrib><creatorcontrib>Wegener, Jörg W.</creatorcontrib><title>Kinetics of relaxation by cGMP/cGKI signaling in fundus smooth muscle</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>cGMP-dependent kinase I (cGKI) is a major mediator of smooth muscle relaxation and exists in two isoforms, α and β. Both isoforms are supposed to mediate their effects via different intracellular signaling pathways. To verify this concept, the kinetics of relaxation mediated by either isoform was analyzed in gastric fundus smooth muscle from mice. Muscles from mice that express selectively the Iα or Iβ isoform of cGKI in smooth muscle (sm-cGKIα or sm-cGKIβ mice) were compared to muscles from conventional cGKI−/− mice. Fundus muscles were contracted by carbachol and then relaxed by 8-Br-cGMP or by electrical field stimulation (EFS). The time course of relaxation by 8-Br-cGMP was not different between muscles from sm-cGKIα and sm-cGKIβ mice. EFS induced a fast transient relaxation in muscles from sm-cGKIα and sm-cGKIβ mice that was blocked by the NO synthase inhibitor L-NAME. Recovery from this relaxation was about 4-times slower in muscles from sm-cGKIα mice than in muscles from sm-cGKIβ mice. The different kinetic of recovery from relaxation after EFS in sm-cGKIα and sm-cGKIβ mice suggests that different signaling pathways exist for each cGKI isoform in vivo in fundus muscles.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>cGMP</subject><subject>Cyclic GMP - analogs &amp; derivatives</subject><subject>Cyclic GMP - metabolism</subject><subject>Cyclic GMP - pharmacology</subject><subject>Electric Stimulation</subject><subject>Female</subject><subject>Fundus</subject><subject>Gastric Fundus</subject><subject>Gene Expression Regulation - drug effects</subject><subject>In Vitro Techniques</subject><subject>Intracellular Signaling Peptides and Proteins - metabolism</subject><subject>Kinetics</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Muscle Relaxation - drug effects</subject><subject>Muscle, Smooth - cytology</subject><subject>Muscle, Smooth - drug effects</subject><subject>Muscle, Smooth - metabolism</subject><subject>Muscle, Smooth - physiology</subject><subject>NANC</subject><subject>Neurons - drug effects</subject><subject>Neurons - metabolism</subject><subject>Nitric oxide</subject><subject>Pharmacology. Drug treatments</subject><subject>Protein Isoforms - metabolism</subject><subject>Relaxation</subject><subject>Signal Transduction - drug effects</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp9kD1PwzAQhi0EgvLxDxDygpgS7pyksRckVEFBFMEAs-XaDrhKnGIniP57UrXAxi23PO97p4eQU4QUAceXi9Qulu8qpAwQUyhTyPkOGSEvRQIlsl0yAsA8YUKIA3IY4wIACsGKfXLAUGA-zIjcPDhvO6cjbSsabK2-VOdaT-crqqePz5d6-nBPo3vzqnb-jTpPq96bPtLYtG33Tps-6toek71K1dGebPcReb29eZncJbOn6f3kepboTGCXKDSAWs2FUFnFh2WAl5mxvADOGeNsXImMmzlwEDnjBdPFGAVTBgvDhKqyI3Kx6V2G9qO3sZONi9rWtfK27aPkYqjKMYeBzDekDm2MwVZyGVyjwkoiyLU_uZAbf3LtT0IpB39D7Gx7oJ831vyGfoQNwPkWUFGrugrKaxf_uHyclRzX3NWGs4OOT2eDjNpZr61xwepOmtb9_8k3yneOPg</recordid><startdate>20111116</startdate><enddate>20111116</enddate><creator>Ertl, Claudia</creator><creator>Lukowski, Robert</creator><creator>Sigl, Katja</creator><creator>Schlossmann, Jens</creator><creator>Hofmann, Franz</creator><creator>Wegener, Jörg W.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20111116</creationdate><title>Kinetics of relaxation by cGMP/cGKI signaling in fundus smooth muscle</title><author>Ertl, Claudia ; Lukowski, Robert ; Sigl, Katja ; Schlossmann, Jens ; Hofmann, Franz ; Wegener, Jörg W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-a1d01cab99a3f8b99d0873de8508822826f938db080942852c56192ad15d29af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>cGMP</topic><topic>Cyclic GMP - analogs &amp; derivatives</topic><topic>Cyclic GMP - metabolism</topic><topic>Cyclic GMP - pharmacology</topic><topic>Electric Stimulation</topic><topic>Female</topic><topic>Fundus</topic><topic>Gastric Fundus</topic><topic>Gene Expression Regulation - drug effects</topic><topic>In Vitro Techniques</topic><topic>Intracellular Signaling Peptides and Proteins - metabolism</topic><topic>Kinetics</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Muscle Relaxation - drug effects</topic><topic>Muscle, Smooth - cytology</topic><topic>Muscle, Smooth - drug effects</topic><topic>Muscle, Smooth - metabolism</topic><topic>Muscle, Smooth - physiology</topic><topic>NANC</topic><topic>Neurons - drug effects</topic><topic>Neurons - metabolism</topic><topic>Nitric oxide</topic><topic>Pharmacology. Drug treatments</topic><topic>Protein Isoforms - metabolism</topic><topic>Relaxation</topic><topic>Signal Transduction - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ertl, Claudia</creatorcontrib><creatorcontrib>Lukowski, Robert</creatorcontrib><creatorcontrib>Sigl, Katja</creatorcontrib><creatorcontrib>Schlossmann, Jens</creatorcontrib><creatorcontrib>Hofmann, Franz</creatorcontrib><creatorcontrib>Wegener, Jörg W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ertl, Claudia</au><au>Lukowski, Robert</au><au>Sigl, Katja</au><au>Schlossmann, Jens</au><au>Hofmann, Franz</au><au>Wegener, Jörg W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kinetics of relaxation by cGMP/cGKI signaling in fundus smooth muscle</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>2011-11-16</date><risdate>2011</risdate><volume>670</volume><issue>1</issue><spage>266</spage><epage>271</epage><pages>266-271</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>cGMP-dependent kinase I (cGKI) is a major mediator of smooth muscle relaxation and exists in two isoforms, α and β. Both isoforms are supposed to mediate their effects via different intracellular signaling pathways. To verify this concept, the kinetics of relaxation mediated by either isoform was analyzed in gastric fundus smooth muscle from mice. Muscles from mice that express selectively the Iα or Iβ isoform of cGKI in smooth muscle (sm-cGKIα or sm-cGKIβ mice) were compared to muscles from conventional cGKI−/− mice. Fundus muscles were contracted by carbachol and then relaxed by 8-Br-cGMP or by electrical field stimulation (EFS). The time course of relaxation by 8-Br-cGMP was not different between muscles from sm-cGKIα and sm-cGKIβ mice. EFS induced a fast transient relaxation in muscles from sm-cGKIα and sm-cGKIβ mice that was blocked by the NO synthase inhibitor L-NAME. Recovery from this relaxation was about 4-times slower in muscles from sm-cGKIα mice than in muscles from sm-cGKIβ mice. The different kinetic of recovery from relaxation after EFS in sm-cGKIα and sm-cGKIβ mice suggests that different signaling pathways exist for each cGKI isoform in vivo in fundus muscles.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>21914444</pmid><doi>10.1016/j.ejphar.2011.07.048</doi><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0014-2999
ispartof European journal of pharmacology, 2011-11, Vol.670 (1), p.266-271
issn 0014-2999
1879-0712
language eng
recordid cdi_proquest_miscellaneous_898504140
source ScienceDirect Journals
subjects Animals
Biological and medical sciences
cGMP
Cyclic GMP - analogs & derivatives
Cyclic GMP - metabolism
Cyclic GMP - pharmacology
Electric Stimulation
Female
Fundus
Gastric Fundus
Gene Expression Regulation - drug effects
In Vitro Techniques
Intracellular Signaling Peptides and Proteins - metabolism
Kinetics
Male
Medical sciences
Mice
Muscle Relaxation - drug effects
Muscle, Smooth - cytology
Muscle, Smooth - drug effects
Muscle, Smooth - metabolism
Muscle, Smooth - physiology
NANC
Neurons - drug effects
Neurons - metabolism
Nitric oxide
Pharmacology. Drug treatments
Protein Isoforms - metabolism
Relaxation
Signal Transduction - drug effects
title Kinetics of relaxation by cGMP/cGKI signaling in fundus smooth muscle
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-11-13T15%3A18%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Kinetics%20of%20relaxation%20by%20cGMP/cGKI%20signaling%20in%20fundus%20smooth%20muscle&rft.jtitle=European%20journal%20of%20pharmacology&rft.au=Ertl,%20Claudia&rft.date=2011-11-16&rft.volume=670&rft.issue=1&rft.spage=266&rft.epage=271&rft.pages=266-271&rft.issn=0014-2999&rft.eissn=1879-0712&rft.coden=EJPHAZ&rft_id=info:doi/10.1016/j.ejphar.2011.07.048&rft_dat=%3Cproquest_cross%3E898504140%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c391t-a1d01cab99a3f8b99d0873de8508822826f938db080942852c56192ad15d29af3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=898504140&rft_id=info:pmid/21914444&rfr_iscdi=true