Chromosome 11q23.1 is an unstable region in B-cell tumor cell lines

Abstract Chromosome 11q23 region is a frequent target of chromosome aberrations in B-cell lymphoid tumors. Here, we present the cytogenetic and molecular characterization of an amplification affecting 11q23.1 in four cell lines derived from B-cell lymphoid tumors. A minimal common region of amplific...

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Bibliographic Details
Published in:Leukemia research 2011-06, Vol.35 (6), p.808-813
Main Authors: Poretti, Giulia, Kwee, Ivo, Bernasconi, Barbara, Rancoita, Paola M.V, Rinaldi, Andrea, Capella, Carlo, Zucca, Emanuele, Neri, Antonino, Tibiletti, Maria Grazia, Bertoni, Francesco
Format: Article
Language:English
Subjects:
B-Lymphocytes - metabolism
B-Lymphocytes - pathology
Cell Line, Tumor
Chromosome Aberrations
Chromosomes, Human, Pair 11 - genetics
FISH, array
Gene Amplification
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Hematology, Oncology and Palliative Medicine
Humans
In Situ Hybridization, Fluorescence
Lymphoma
Neoplasms - genetics
Neoplasms - pathology
Oligonucleotide Array Sequence Analysis
POUAF1
PPP2R1B
Protein Phosphatase 2 - genetics
Protein-Serine-Threonine Kinases - genetics
Reverse Transcriptase Polymerase Chain Reaction
SIK2
Tiling
Trans-Activators - genetics
Transcriptome
Translocation
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Summary:Abstract Chromosome 11q23 region is a frequent target of chromosome aberrations in B-cell lymphoid tumors. Here, we present the cytogenetic and molecular characterization of an amplification affecting 11q23.1 in four cell lines derived from B-cell lymphoid tumors. A minimal common region of amplification of 330 kb was identified in three cell lines using Affymetrix Human Mapping 250K arrays. When analyzed with three BAC clones, the amplifications appeared different at cytogenetic level in each cell line. Possibly affected transcripts were evaluated using tiling arrays, and validated by real time PCR. Since no effect of the amplification at the local transcription level was observed, it is possible that 11q23 amplification might mainly represent the effect of unstable chromosomal region.
ISSN:0145-2126
1873-5835
DOI:10.1016/j.leukres.2010.10.032