Generation of reactive oxygen species from porous silicon microparticles in cell culture medium

Nanostructured (porous) silicon is a promising biodegradable biomaterial, which is being intensively researched as a tissue engineering scaffold and drug‐delivery vehicle. Here, we tested the biocompatibility of non‐treated and thermally‐oxidized porous silicon particles using an indirect cell viabi...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2010-06, Vol.93A (3), p.1124-1131
Main Authors: Low, Suet Peng, Williams, Keryn A., Canham, Leigh T., Voelcker, Nicolas H.
Format: Article
Language:English
Subjects:
Biocompatibility
biodegradable materials
Biological and medical sciences
Biomedical materials
Cell Survival - drug effects
Cells, Cultured
Culture
Culture Media - pharmacology
Epithelial Cells - cytology
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Fluoresceins - metabolism
Humans
Lens, Crystalline - cytology
Medical sciences
Membranes, Artificial
Microparticles
Nanoparticles - chemistry
Porosity - drug effects
Porous silicon
reactive oxygen species
Reactive Oxygen Species - metabolism
Silicon - pharmacology
Spectroscopy, Fourier Transform Infrared
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Surgical implants
Technology. Biomaterials. Equipments
Toxic
Viability
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Summary:Nanostructured (porous) silicon is a promising biodegradable biomaterial, which is being intensively researched as a tissue engineering scaffold and drug‐delivery vehicle. Here, we tested the biocompatibility of non‐treated and thermally‐oxidized porous silicon particles using an indirect cell viability assay. Initial direct cell culture on porous silicon determined that human lens epithelial cells only poorly adhered to non‐treated porous silicon. Using an indirect cell culture assay, we found that non‐treated microparticles caused complete cell death, indicating that these particles generated a toxic product in cell culture medium. In contrast, thermally‐oxidized microparticles did not reduce cell viability significantly. We found evidence for the generation of reactive oxygen species (ROS) by means of the fluorescent probe 2′,7′‐dichlorofluorescin. Our results suggest that non‐treated porous silicon microparticles produced ROS, which interacted with the components of the cell culture medium, leading to the formation of cytotoxic species. Oxidation of porous silicon microparticles not only mitigated, but also abolished the toxic effects. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
ISSN:1549-3296
1552-4965
1552-4965
DOI:10.1002/jbm.a.32610