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Dormancy of Medicago marina (L.) seed
► Medicago marina seed dormancy is due to the presence of a hard seed coat. ► Seed-coat dormancy is removed by scarification and freezing, but not by heating. ► Seed might differently modulate ABA content in response to stress and germination. ► Seed have complex mechanism to control dormancy and to...
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| Published in: | Environmental and experimental botany 2011-09, Vol.72 (2), p.320-329 |
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| Main Authors: | , , , , , , |
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| cited_by | cdi_FETCH-LOGICAL-c377t-b8bc8c91121fdf40cdfb0de28eba744a26b0704b439543c6900b8eb74365e73c3 |
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| cites | cdi_FETCH-LOGICAL-c377t-b8bc8c91121fdf40cdfb0de28eba744a26b0704b439543c6900b8eb74365e73c3 |
| container_end_page | 329 |
| container_issue | 2 |
| container_start_page | 320 |
| container_title | Environmental and experimental botany |
| container_volume | 72 |
| creator | Scippa, G.S. Petrollini, E. Trupiano, D. Rocco, M. Falco, G. Di Michele, M. Chiatante, D. |
| description | ►
Medicago marina seed dormancy is due to the presence of a hard seed coat. ► Seed-coat dormancy is removed by scarification and freezing, but not by heating. ► Seed might differently modulate ABA content in response to stress and germination. ► Seed have complex mechanism to control dormancy and to protect cellular structures.
Medicago marina (L.) is a Mediterranean species whose seeds show strong dormancy that prevents germination. We used an integrated approach of physiological analyses and proteomics to investigate the mechanisms that control
M. marina dormancy/germination and that underlie stress tolerance. First, we evaluated the effects on dormancy breaking of the following treatments: mechanical scarification, freezing at −20
°C, storage for 4 months and heating at 100
°C for 1
h. Mechanical scarification and freezing were the most effective treatments in overcoming dormancy. The role of abscisic acid (ABA) in
M. marina dormancy was studied by ELISA immuno-enzymatic assay. The ABA content of germinated and non-germinated mature (control) and treated seeds was determined. The level of ABA was higher in treated seeds than in control seeds; the most significant increase occurred in the heated seeds. A comparison of the ABA level in the germinated, control and treated seeds suggests that different mechanisms modulate ABA content in response to different stresses, and that a specific ABA-signalling pathway regulates germination. Proteomic analysis revealed 46 proteins differentially expressed between treated and untreated seeds; 14 of these proteins were subsequently identified by mass spectrometry. Several of the proteins identified are important factors in the stress response, and are involved in such diverse functions as lipid metabolism, protein folding and chromatin protection. Lastly, an analysis of the phosphoproteome maps showed that the function of many proteins in seeds subjected to temperature treatment is modulated through post-translational modifications. |
| doi_str_mv | 10.1016/j.envexpbot.2011.04.005 |
| format | article |
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Medicago marina seed dormancy is due to the presence of a hard seed coat. ► Seed-coat dormancy is removed by scarification and freezing, but not by heating. ► Seed might differently modulate ABA content in response to stress and germination. ► Seed have complex mechanism to control dormancy and to protect cellular structures.
Medicago marina (L.) is a Mediterranean species whose seeds show strong dormancy that prevents germination. We used an integrated approach of physiological analyses and proteomics to investigate the mechanisms that control
M. marina dormancy/germination and that underlie stress tolerance. First, we evaluated the effects on dormancy breaking of the following treatments: mechanical scarification, freezing at −20
°C, storage for 4 months and heating at 100
°C for 1
h. Mechanical scarification and freezing were the most effective treatments in overcoming dormancy. The role of abscisic acid (ABA) in
M. marina dormancy was studied by ELISA immuno-enzymatic assay. The ABA content of germinated and non-germinated mature (control) and treated seeds was determined. The level of ABA was higher in treated seeds than in control seeds; the most significant increase occurred in the heated seeds. A comparison of the ABA level in the germinated, control and treated seeds suggests that different mechanisms modulate ABA content in response to different stresses, and that a specific ABA-signalling pathway regulates germination. Proteomic analysis revealed 46 proteins differentially expressed between treated and untreated seeds; 14 of these proteins were subsequently identified by mass spectrometry. Several of the proteins identified are important factors in the stress response, and are involved in such diverse functions as lipid metabolism, protein folding and chromatin protection. Lastly, an analysis of the phosphoproteome maps showed that the function of many proteins in seeds subjected to temperature treatment is modulated through post-translational modifications.</description><identifier>ISSN: 0098-8472</identifier><identifier>EISSN: 1873-7307</identifier><identifier>DOI: 10.1016/j.envexpbot.2011.04.005</identifier><identifier>CODEN: EEBODM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Abscisic acid ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Germination and dormancy ; Medicago ; Medicago marina ; Physical dormancy ; Plant physiology and development ; Proteomics</subject><ispartof>Environmental and experimental botany, 2011-09, Vol.72 (2), p.320-329</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-b8bc8c91121fdf40cdfb0de28eba744a26b0704b439543c6900b8eb74365e73c3</citedby><cites>FETCH-LOGICAL-c377t-b8bc8c91121fdf40cdfb0de28eba744a26b0704b439543c6900b8eb74365e73c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24299343$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Scippa, G.S.</creatorcontrib><creatorcontrib>Petrollini, E.</creatorcontrib><creatorcontrib>Trupiano, D.</creatorcontrib><creatorcontrib>Rocco, M.</creatorcontrib><creatorcontrib>Falco, G.</creatorcontrib><creatorcontrib>Di Michele, M.</creatorcontrib><creatorcontrib>Chiatante, D.</creatorcontrib><title>Dormancy of Medicago marina (L.) seed</title><title>Environmental and experimental botany</title><description>►
Medicago marina seed dormancy is due to the presence of a hard seed coat. ► Seed-coat dormancy is removed by scarification and freezing, but not by heating. ► Seed might differently modulate ABA content in response to stress and germination. ► Seed have complex mechanism to control dormancy and to protect cellular structures.
Medicago marina (L.) is a Mediterranean species whose seeds show strong dormancy that prevents germination. We used an integrated approach of physiological analyses and proteomics to investigate the mechanisms that control
M. marina dormancy/germination and that underlie stress tolerance. First, we evaluated the effects on dormancy breaking of the following treatments: mechanical scarification, freezing at −20
°C, storage for 4 months and heating at 100
°C for 1
h. Mechanical scarification and freezing were the most effective treatments in overcoming dormancy. The role of abscisic acid (ABA) in
M. marina dormancy was studied by ELISA immuno-enzymatic assay. The ABA content of germinated and non-germinated mature (control) and treated seeds was determined. The level of ABA was higher in treated seeds than in control seeds; the most significant increase occurred in the heated seeds. A comparison of the ABA level in the germinated, control and treated seeds suggests that different mechanisms modulate ABA content in response to different stresses, and that a specific ABA-signalling pathway regulates germination. Proteomic analysis revealed 46 proteins differentially expressed between treated and untreated seeds; 14 of these proteins were subsequently identified by mass spectrometry. Several of the proteins identified are important factors in the stress response, and are involved in such diverse functions as lipid metabolism, protein folding and chromatin protection. Lastly, an analysis of the phosphoproteome maps showed that the function of many proteins in seeds subjected to temperature treatment is modulated through post-translational modifications.</description><subject>Abscisic acid</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Germination and dormancy</subject><subject>Medicago</subject><subject>Medicago marina</subject><subject>Physical dormancy</subject><subject>Plant physiology and development</subject><subject>Proteomics</subject><issn>0098-8472</issn><issn>1873-7307</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqFkM1OwzAQhC0EEqXwDORSAYeEdezGzrHiXyriAmfLdjbIVRoXO63o2-OqVa-c9rAzOzsfIdcUCgq0ul8U2G_wd2X8UJRAaQG8AJiekBGVguWCgTglI4Ba5pKL8pxcxLgAAMFENSKTRx-WurfbzLfZOzbO6m-fLXVwvc5u58VdFhGbS3LW6i7i1WGOydfz0-fDaz7_eHl7mM1zy4QYciONlbamtKRt03KwTWugwVKi0YJzXVYGBHDDWT3lzFY1gEk7wVk1RcEsG5Ob_d1V8D9rjINaumix63SPfh2VFJzKqhaQlGKvtMHHGLBVq-DS21tFQe24qIU6clE7Lgq4SlySc3LI0NHqrg2pvYtHe8nLumacJd1sr8NUeOMwqGgd9jYxCmgH1Xj3b9YfHOx7CA</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Scippa, G.S.</creator><creator>Petrollini, E.</creator><creator>Trupiano, D.</creator><creator>Rocco, M.</creator><creator>Falco, G.</creator><creator>Di Michele, M.</creator><creator>Chiatante, D.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7U6</scope><scope>C1K</scope><scope>SOI</scope></search><sort><creationdate>20110901</creationdate><title>Dormancy of Medicago marina (L.) seed</title><author>Scippa, G.S. ; Petrollini, E. ; Trupiano, D. ; Rocco, M. ; Falco, G. ; Di Michele, M. ; Chiatante, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-b8bc8c91121fdf40cdfb0de28eba744a26b0704b439543c6900b8eb74365e73c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Abscisic acid</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Germination and dormancy</topic><topic>Medicago</topic><topic>Medicago marina</topic><topic>Physical dormancy</topic><topic>Plant physiology and development</topic><topic>Proteomics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Scippa, G.S.</creatorcontrib><creatorcontrib>Petrollini, E.</creatorcontrib><creatorcontrib>Trupiano, D.</creatorcontrib><creatorcontrib>Rocco, M.</creatorcontrib><creatorcontrib>Falco, G.</creatorcontrib><creatorcontrib>Di Michele, M.</creatorcontrib><creatorcontrib>Chiatante, D.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Sustainability Science Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><jtitle>Environmental and experimental botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Scippa, G.S.</au><au>Petrollini, E.</au><au>Trupiano, D.</au><au>Rocco, M.</au><au>Falco, G.</au><au>Di Michele, M.</au><au>Chiatante, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dormancy of Medicago marina (L.) seed</atitle><jtitle>Environmental and experimental botany</jtitle><date>2011-09-01</date><risdate>2011</risdate><volume>72</volume><issue>2</issue><spage>320</spage><epage>329</epage><pages>320-329</pages><issn>0098-8472</issn><eissn>1873-7307</eissn><coden>EEBODM</coden><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>►
Medicago marina seed dormancy is due to the presence of a hard seed coat. ► Seed-coat dormancy is removed by scarification and freezing, but not by heating. ► Seed might differently modulate ABA content in response to stress and germination. ► Seed have complex mechanism to control dormancy and to protect cellular structures.
Medicago marina (L.) is a Mediterranean species whose seeds show strong dormancy that prevents germination. We used an integrated approach of physiological analyses and proteomics to investigate the mechanisms that control
M. marina dormancy/germination and that underlie stress tolerance. First, we evaluated the effects on dormancy breaking of the following treatments: mechanical scarification, freezing at −20
°C, storage for 4 months and heating at 100
°C for 1
h. Mechanical scarification and freezing were the most effective treatments in overcoming dormancy. The role of abscisic acid (ABA) in
M. marina dormancy was studied by ELISA immuno-enzymatic assay. The ABA content of germinated and non-germinated mature (control) and treated seeds was determined. The level of ABA was higher in treated seeds than in control seeds; the most significant increase occurred in the heated seeds. A comparison of the ABA level in the germinated, control and treated seeds suggests that different mechanisms modulate ABA content in response to different stresses, and that a specific ABA-signalling pathway regulates germination. Proteomic analysis revealed 46 proteins differentially expressed between treated and untreated seeds; 14 of these proteins were subsequently identified by mass spectrometry. Several of the proteins identified are important factors in the stress response, and are involved in such diverse functions as lipid metabolism, protein folding and chromatin protection. Lastly, an analysis of the phosphoproteome maps showed that the function of many proteins in seeds subjected to temperature treatment is modulated through post-translational modifications.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.envexpbot.2011.04.005</doi><tpages>10</tpages></addata></record> |
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| ispartof | Environmental and experimental botany, 2011-09, Vol.72 (2), p.320-329 |
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| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Abscisic acid Biological and medical sciences Fundamental and applied biological sciences. Psychology Germination and dormancy Medicago Medicago marina Physical dormancy Plant physiology and development Proteomics |
| title | Dormancy of Medicago marina (L.) seed |
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