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Distribution and transferability of tetracycline resistance determinants in Escherichia coli isolated from meat and meat products
Escherichia coli is used to assess the hygienic quality of food products and the dissemination of antimicrobial resistance. In particular, tetracycline-resistant E. coli can be chosen as an indicator of antibiotic resistant bacteria because it has a high frequency of occurrence. The purpose of this...
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| Published in: | International journal of food microbiology 2011-02, Vol.145 (2), p.407-413 |
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| creator | Koo, Hyon-Ji Woo, Gun-Jo |
| description | Escherichia coli is used to assess the hygienic quality of food products and the dissemination of antimicrobial resistance. In particular, tetracycline-resistant
E. coli can be chosen as an indicator of antibiotic resistant bacteria because it has a high frequency of occurrence. The purpose of this study was to investigate the distribution and transfer of tetracycline resistance determinants in meatborne
E. coli. A total of 121 tetracycline-resistant
E. coli isolates were collected from meat and meat products (raw meat, fish, and processed foods) from 2004 to 2006 in Korea. Among these isolates,
tet(A) (52.4%) was the most frequent tetracycline resistance determinant, followed by
tet(B) (41.3%), whereas
tet(C) (1.7%) and
tet(D) (0.8%) were less frequently identified. Two isolates (1.6%) contained two
tet genes simultaneously,
tet(A) and
tet(B). Minimal inhibitory concentrations (MICs) to tetracycline family antibiotics, such as tetracycline, minocycline, doxycycline, oxytetracycline, and chlortetracycline were higher for isolates carrying the
tet(B) gene compared to isolates carrying
tet(A) (
P
<
0.0001). Conjugation experiments were performed by the broth mating method; 119 isolates (98.3%) containing at least one of the
tet genes were shown to transfer tetracycline resistance to recipient
E. coli J53. Also, we observed high diversity of tetracycline-resistant
E. coli isolates in meat and meat products in Korea by using
XbaI pulsed-field gel electrophoresis (PFGE) typing. This study suggests that the high prevalence of tetracycline-resistant
E. coli in meat may be due to the high transferability of
tet determinants.
► We investigate the distribution and transfer of
tet genes in meatborne
E. coli.
► tet(A) was the most frequent, followed by
tet(B),
tet(C) and
tet(D). ► MIC to tetracyclines were higher for
E. coli carrying
tetB than
E. coli carrying
tetA. ► Most
tet genes found in our study were located on transferable elements. |
| doi_str_mv | 10.1016/j.ijfoodmicro.2011.01.003 |
| format | article |
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E. coli can be chosen as an indicator of antibiotic resistant bacteria because it has a high frequency of occurrence. The purpose of this study was to investigate the distribution and transfer of tetracycline resistance determinants in meatborne
E. coli. A total of 121 tetracycline-resistant
E. coli isolates were collected from meat and meat products (raw meat, fish, and processed foods) from 2004 to 2006 in Korea. Among these isolates,
tet(A) (52.4%) was the most frequent tetracycline resistance determinant, followed by
tet(B) (41.3%), whereas
tet(C) (1.7%) and
tet(D) (0.8%) were less frequently identified. Two isolates (1.6%) contained two
tet genes simultaneously,
tet(A) and
tet(B). Minimal inhibitory concentrations (MICs) to tetracycline family antibiotics, such as tetracycline, minocycline, doxycycline, oxytetracycline, and chlortetracycline were higher for isolates carrying the
tet(B) gene compared to isolates carrying
tet(A) (
P
<
0.0001). Conjugation experiments were performed by the broth mating method; 119 isolates (98.3%) containing at least one of the
tet genes were shown to transfer tetracycline resistance to recipient
E. coli J53. Also, we observed high diversity of tetracycline-resistant
E. coli isolates in meat and meat products in Korea by using
XbaI pulsed-field gel electrophoresis (PFGE) typing. This study suggests that the high prevalence of tetracycline-resistant
E. coli in meat may be due to the high transferability of
tet determinants.
► We investigate the distribution and transfer of
tet genes in meatborne
E. coli.
► tet(A) was the most frequent, followed by
tet(B),
tet(C) and
tet(D). ► MIC to tetracyclines were higher for
E. coli carrying
tetB than
E. coli carrying
tetA. ► Most
tet genes found in our study were located on transferable elements.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2011.01.003</identifier><identifier>PMID: 21324543</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Anti-Bacterial Agents - pharmacology ; Antimicrobial resistance ; Conjugation, Genetic ; E. coli ; Electrophoresis, Gel, Pulsed-Field ; Escherichia coli ; Escherichia coli - drug effects ; Escherichia coli - genetics ; Escherichia coli - isolation & purification ; Escherichia coli Proteins - genetics ; Genes, Bacterial ; Meat - microbiology ; Meat Products - microbiology ; Microbial Sensitivity Tests ; PFGE ; Phenotype ; Republic of Korea ; Seafood - microbiology ; tet ; Tetracycline resistance ; Tetracycline Resistance - genetics ; Tetracyclines - pharmacology</subject><ispartof>International journal of food microbiology, 2011-02, Vol.145 (2), p.407-413</ispartof><rights>2011 Elsevier B.V.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-1d1879d48bc69dd4befa36d5fb1f02bfec47f88a620bc1001a1ec184a2764f9c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,783,787,27936,27937</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21324543$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koo, Hyon-Ji</creatorcontrib><creatorcontrib>Woo, Gun-Jo</creatorcontrib><title>Distribution and transferability of tetracycline resistance determinants in Escherichia coli isolated from meat and meat products</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>Escherichia coli is used to assess the hygienic quality of food products and the dissemination of antimicrobial resistance. In particular, tetracycline-resistant
E. coli can be chosen as an indicator of antibiotic resistant bacteria because it has a high frequency of occurrence. The purpose of this study was to investigate the distribution and transfer of tetracycline resistance determinants in meatborne
E. coli. A total of 121 tetracycline-resistant
E. coli isolates were collected from meat and meat products (raw meat, fish, and processed foods) from 2004 to 2006 in Korea. Among these isolates,
tet(A) (52.4%) was the most frequent tetracycline resistance determinant, followed by
tet(B) (41.3%), whereas
tet(C) (1.7%) and
tet(D) (0.8%) were less frequently identified. Two isolates (1.6%) contained two
tet genes simultaneously,
tet(A) and
tet(B). Minimal inhibitory concentrations (MICs) to tetracycline family antibiotics, such as tetracycline, minocycline, doxycycline, oxytetracycline, and chlortetracycline were higher for isolates carrying the
tet(B) gene compared to isolates carrying
tet(A) (
P
<
0.0001). Conjugation experiments were performed by the broth mating method; 119 isolates (98.3%) containing at least one of the
tet genes were shown to transfer tetracycline resistance to recipient
E. coli J53. Also, we observed high diversity of tetracycline-resistant
E. coli isolates in meat and meat products in Korea by using
XbaI pulsed-field gel electrophoresis (PFGE) typing. This study suggests that the high prevalence of tetracycline-resistant
E. coli in meat may be due to the high transferability of
tet determinants.
► We investigate the distribution and transfer of
tet genes in meatborne
E. coli.
► tet(A) was the most frequent, followed by
tet(B),
tet(C) and
tet(D). ► MIC to tetracyclines were higher for
E. coli carrying
tetB than
E. coli carrying
tetA. ► Most
tet genes found in our study were located on transferable elements.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antimicrobial resistance</subject><subject>Conjugation, Genetic</subject><subject>E. coli</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Escherichia coli</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - isolation & purification</subject><subject>Escherichia coli Proteins - genetics</subject><subject>Genes, Bacterial</subject><subject>Meat - microbiology</subject><subject>Meat Products - microbiology</subject><subject>Microbial Sensitivity Tests</subject><subject>PFGE</subject><subject>Phenotype</subject><subject>Republic of Korea</subject><subject>Seafood - microbiology</subject><subject>tet</subject><subject>Tetracycline resistance</subject><subject>Tetracycline Resistance - genetics</subject><subject>Tetracyclines - pharmacology</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqNkU2PFCEQhonRuLOrf0Hx5KnHoqG_jmZcV5NNPOieCQ2FW5PuZgV6kzn6z2V2VuNNk0og5Kl6gYexNwK2AkT7br-lvQ_BzWRj2NYgxBZKgXzCNqLvhkqqFp6yTWH7SrTQnLHzlPYA0EgJz9lZLWStGiU37OcHSjnSuGYKCzeL4zmaJXmMZqSJ8oEHzzOWQ3uwEy3II6bSYhaL3GHGONNilpw4Lfwy2VuMZG_JcBsm4pTCZDI67mOY-YwmP0Q8bO5icKvN6QV75s2U8OXjesFuPl5-232qrr9cfd69v66sGvpcCXd8mVP9aNvBOTWiN7J1jR-Fh3r0aFXn-960NYxWAAgj0IpembprlR-svGBvT3NL8I8VU9YzJYvTZBYMa9J9W3e9HFT7b7JpBhiGVhVyOJHFQ0oRvb6LNJt40AL0UZXe679U6aMqDaVAlt5XjynrOKP70_nbTQFenwBvgjbfIyV987VMkCCGRnQCCrE7EVj-7Z4w6mQJixlHEW3WLtB_XOQXxpu4Cg</recordid><startdate>20110228</startdate><enddate>20110228</enddate><creator>Koo, Hyon-Ji</creator><creator>Woo, Gun-Jo</creator><general>Elsevier B.V</general><general>[Amsterdam; New York, NY]: Elsevier Science</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T2</scope><scope>7T7</scope><scope>7U2</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20110228</creationdate><title>Distribution and transferability of tetracycline resistance determinants in Escherichia coli isolated from meat and meat products</title><author>Koo, Hyon-Ji ; Woo, Gun-Jo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c498t-1d1879d48bc69dd4befa36d5fb1f02bfec47f88a620bc1001a1ec184a2764f9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antimicrobial resistance</topic><topic>Conjugation, Genetic</topic><topic>E. coli</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Escherichia coli</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - isolation & purification</topic><topic>Escherichia coli Proteins - genetics</topic><topic>Genes, Bacterial</topic><topic>Meat - microbiology</topic><topic>Meat Products - microbiology</topic><topic>Microbial Sensitivity Tests</topic><topic>PFGE</topic><topic>Phenotype</topic><topic>Republic of Korea</topic><topic>Seafood - microbiology</topic><topic>tet</topic><topic>Tetracycline resistance</topic><topic>Tetracycline Resistance - genetics</topic><topic>Tetracyclines - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koo, Hyon-Ji</creatorcontrib><creatorcontrib>Woo, Gun-Jo</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Safety Science and Risk</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koo, Hyon-Ji</au><au>Woo, Gun-Jo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution and transferability of tetracycline resistance determinants in Escherichia coli isolated from meat and meat products</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2011-02-28</date><risdate>2011</risdate><volume>145</volume><issue>2</issue><spage>407</spage><epage>413</epage><pages>407-413</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><abstract>Escherichia coli is used to assess the hygienic quality of food products and the dissemination of antimicrobial resistance. In particular, tetracycline-resistant
E. coli can be chosen as an indicator of antibiotic resistant bacteria because it has a high frequency of occurrence. The purpose of this study was to investigate the distribution and transfer of tetracycline resistance determinants in meatborne
E. coli. A total of 121 tetracycline-resistant
E. coli isolates were collected from meat and meat products (raw meat, fish, and processed foods) from 2004 to 2006 in Korea. Among these isolates,
tet(A) (52.4%) was the most frequent tetracycline resistance determinant, followed by
tet(B) (41.3%), whereas
tet(C) (1.7%) and
tet(D) (0.8%) were less frequently identified. Two isolates (1.6%) contained two
tet genes simultaneously,
tet(A) and
tet(B). Minimal inhibitory concentrations (MICs) to tetracycline family antibiotics, such as tetracycline, minocycline, doxycycline, oxytetracycline, and chlortetracycline were higher for isolates carrying the
tet(B) gene compared to isolates carrying
tet(A) (
P
<
0.0001). Conjugation experiments were performed by the broth mating method; 119 isolates (98.3%) containing at least one of the
tet genes were shown to transfer tetracycline resistance to recipient
E. coli J53. Also, we observed high diversity of tetracycline-resistant
E. coli isolates in meat and meat products in Korea by using
XbaI pulsed-field gel electrophoresis (PFGE) typing. This study suggests that the high prevalence of tetracycline-resistant
E. coli in meat may be due to the high transferability of
tet determinants.
► We investigate the distribution and transfer of
tet genes in meatborne
E. coli.
► tet(A) was the most frequent, followed by
tet(B),
tet(C) and
tet(D). ► MIC to tetracyclines were higher for
E. coli carrying
tetB than
E. coli carrying
tetA. ► Most
tet genes found in our study were located on transferable elements.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>21324543</pmid><doi>10.1016/j.ijfoodmicro.2011.01.003</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1605 |
| ispartof | International journal of food microbiology, 2011-02, Vol.145 (2), p.407-413 |
| issn | 0168-1605 1879-3460 |
| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Anti-Bacterial Agents - pharmacology Antimicrobial resistance Conjugation, Genetic E. coli Electrophoresis, Gel, Pulsed-Field Escherichia coli Escherichia coli - drug effects Escherichia coli - genetics Escherichia coli - isolation & purification Escherichia coli Proteins - genetics Genes, Bacterial Meat - microbiology Meat Products - microbiology Microbial Sensitivity Tests PFGE Phenotype Republic of Korea Seafood - microbiology tet Tetracycline resistance Tetracycline Resistance - genetics Tetracyclines - pharmacology |
| title | Distribution and transferability of tetracycline resistance determinants in Escherichia coli isolated from meat and meat products |
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