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Physiological optimization underlies growth rate-independent chlorophyll-specific gross and net primary production
Characterization of physiological variability in phytoplankton photosynthetic efficiencies is one of the greatest challenges in assessing ocean net primary production (NPP) from remote sensing of surface chlorophyll (Chl). Nutrient limitation strongly influences phytoplankton intracellular pigmentat...
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Published in: | Photosynthesis research 2010-02, Vol.103 (2), p.125-137 |
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description | Characterization of physiological variability in phytoplankton photosynthetic efficiencies is one of the greatest challenges in assessing ocean net primary production (NPP) from remote sensing of surface chlorophyll (Chl). Nutrient limitation strongly influences phytoplankton intracellular pigmentation, but its impact on Chl-specific NPP (NPP *) is debated. We monitored six indices of photosynthetic activity in steady-state Dunaliella tertiolecta cultures over a range of nitrate-limited growth rates (μ), including photosynthetic efficiency of PSII (F v/F m), O₂-based gross and net production, 20 min and 24 h carbon assimilation, and carbon- and μ-based NPP. Across all growth rates, O₂-based Chl-specific gross primary production ( [graphic removed] ), NPP *, and F v/F m were constant. [graphic removed] was 3.3 times greater than NPP *. In stark contrast, Chl-specific short-term C fixation showed clear linear dependence on μ, reflecting differential allocation of photosynthate between short-lived C products and longer-term storage products. Indeed, ¹⁴C incorporation into carbohydrates was five times greater in cells growing at 1.2 day⁻¹ than 0.12 day⁻¹. These storage products are catabolized for ATP and reductant generation within the period of a cell cycle. The relationship between Chl-specific gross and net O₂ production, short-term ¹⁴C-uptake, NPP *, and growth rate reflects cellular-level regulation of fundamental metabolic pathways in response to nutrient limitation. We conclude that growth rate-dependent photosynthate metabolism bridges the gap between gross and net production and resolves a controversial question regarding nutrient limitation effects on primary production measures. |
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Nutrient limitation strongly influences phytoplankton intracellular pigmentation, but its impact on Chl-specific NPP (NPP *) is debated. We monitored six indices of photosynthetic activity in steady-state Dunaliella tertiolecta cultures over a range of nitrate-limited growth rates (μ), including photosynthetic efficiency of PSII (F v/F m), O₂-based gross and net production, 20 min and 24 h carbon assimilation, and carbon- and μ-based NPP. Across all growth rates, O₂-based Chl-specific gross primary production ( [graphic removed] ), NPP *, and F v/F m were constant. [graphic removed] was 3.3 times greater than NPP *. In stark contrast, Chl-specific short-term C fixation showed clear linear dependence on μ, reflecting differential allocation of photosynthate between short-lived C products and longer-term storage products. Indeed, ¹⁴C incorporation into carbohydrates was five times greater in cells growing at 1.2 day⁻¹ than 0.12 day⁻¹. These storage products are catabolized for ATP and reductant generation within the period of a cell cycle. The relationship between Chl-specific gross and net O₂ production, short-term ¹⁴C-uptake, NPP *, and growth rate reflects cellular-level regulation of fundamental metabolic pathways in response to nutrient limitation. We conclude that growth rate-dependent photosynthate metabolism bridges the gap between gross and net production and resolves a controversial question regarding nutrient limitation effects on primary production measures.</description><identifier>ISSN: 0166-8595</identifier><identifier>EISSN: 1573-5079</identifier><identifier>DOI: 10.1007/s11120-009-9526-z</identifier><identifier>PMID: 20066494</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Carbon - metabolism ; Chlorophyll ; Chlorophyll - metabolism ; Kinetics ; Life Sciences ; Light ; Nitrates - metabolism ; Oxygen - metabolism ; Photosynthesis ; Photosynthesis - physiology ; Photosynthesis - radiation effects ; Physiological aspects ; Phytoplankton - growth & development ; Phytoplankton - metabolism ; Phytoplankton - radiation effects ; Plant Genetics and Genomics ; Plant Physiology ; Plant Sciences ; Regular Paper ; Remote sensing ; Time Factors ; Toy industry</subject><ispartof>Photosynthesis research, 2010-02, Vol.103 (2), p.125-137</ispartof><rights>Springer Science+Business Media B.V. 2010</rights><rights>COPYRIGHT 2010 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c565t-7bced1209aaf16568a76303e801098074a8f847f6d4fd0ddf03cc5e44bdadc7f3</citedby><cites>FETCH-LOGICAL-c565t-7bced1209aaf16568a76303e801098074a8f847f6d4fd0ddf03cc5e44bdadc7f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20066494$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Halsey, Kimberly H</creatorcontrib><creatorcontrib>Milligan, Allen J</creatorcontrib><creatorcontrib>Behrenfeld, Michael J</creatorcontrib><title>Physiological optimization underlies growth rate-independent chlorophyll-specific gross and net primary production</title><title>Photosynthesis research</title><addtitle>Photosynth Res</addtitle><addtitle>Photosynth Res</addtitle><description>Characterization of physiological variability in phytoplankton photosynthetic efficiencies is one of the greatest challenges in assessing ocean net primary production (NPP) from remote sensing of surface chlorophyll (Chl). Nutrient limitation strongly influences phytoplankton intracellular pigmentation, but its impact on Chl-specific NPP (NPP *) is debated. We monitored six indices of photosynthetic activity in steady-state Dunaliella tertiolecta cultures over a range of nitrate-limited growth rates (μ), including photosynthetic efficiency of PSII (F v/F m), O₂-based gross and net production, 20 min and 24 h carbon assimilation, and carbon- and μ-based NPP. Across all growth rates, O₂-based Chl-specific gross primary production ( [graphic removed] ), NPP *, and F v/F m were constant. [graphic removed] was 3.3 times greater than NPP *. In stark contrast, Chl-specific short-term C fixation showed clear linear dependence on μ, reflecting differential allocation of photosynthate between short-lived C products and longer-term storage products. Indeed, ¹⁴C incorporation into carbohydrates was five times greater in cells growing at 1.2 day⁻¹ than 0.12 day⁻¹. These storage products are catabolized for ATP and reductant generation within the period of a cell cycle. The relationship between Chl-specific gross and net O₂ production, short-term ¹⁴C-uptake, NPP *, and growth rate reflects cellular-level regulation of fundamental metabolic pathways in response to nutrient limitation. We conclude that growth rate-dependent photosynthate metabolism bridges the gap between gross and net production and resolves a controversial question regarding nutrient limitation effects on primary production measures.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Carbon - metabolism</subject><subject>Chlorophyll</subject><subject>Chlorophyll - metabolism</subject><subject>Kinetics</subject><subject>Life Sciences</subject><subject>Light</subject><subject>Nitrates - metabolism</subject><subject>Oxygen - metabolism</subject><subject>Photosynthesis</subject><subject>Photosynthesis - physiology</subject><subject>Photosynthesis - radiation effects</subject><subject>Physiological aspects</subject><subject>Phytoplankton - growth & development</subject><subject>Phytoplankton - metabolism</subject><subject>Phytoplankton - radiation effects</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Regular Paper</subject><subject>Remote sensing</subject><subject>Time Factors</subject><subject>Toy industry</subject><issn>0166-8595</issn><issn>1573-5079</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFkstu1DAUhiMEokPhAdhAxAaxSDmOb8myqrhUqgSidG15fMm4SuJgOyozT49DBlBZgCzZ0vH3n5v-oniO4AwB8LcRIVRDBdBWLa1ZdXhQbBDluKLA24fFBhBjVUNbelI8ifEWABqG8OPipAZgjLRkU4TPu310vvedU7Iv_ZTc4A4yOT-W86hN6J2JZRf8XdqVQSZTuRydTL7GVKpd74Ofdvu-r-JklLNOLXCMpRx1OZpUTsENMuzz6_WslrxPi0dW9tE8O76nxc37d18vPlZXnz5cXpxfVYoymiq-VUbn8VopLWKUNZIzDNg0gKBtgBPZ2IZwyzSxGrS2gJWihpCtllpxi0-L12veXPrbbGISg4vK9L0cjZ-jyLugbc0I_i_JCatzUcYz-eov8tbPYcxjiDq3mvtEdYbOVqiTvRFutD4FqfLRZnDKj8a6HD_HrMak4T8Fb-4JMpPM99TJOUZxef3lPotWVi1rDsaK44YFArG4QqyuENkVYnGFOGTNi2PX83Yw-rfilw0yUK9AzF9jZ8Kfsf6V9eUqstIL2QUXxc11DQgD4i2hgPEPLLjNZw</recordid><startdate>20100201</startdate><enddate>20100201</enddate><creator>Halsey, Kimberly H</creator><creator>Milligan, Allen J</creator><creator>Behrenfeld, Michael J</creator><general>Dordrecht : Springer Netherlands</general><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QP</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20100201</creationdate><title>Physiological optimization underlies growth rate-independent chlorophyll-specific gross and net primary production</title><author>Halsey, Kimberly H ; Milligan, Allen J ; Behrenfeld, Michael J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c565t-7bced1209aaf16568a76303e801098074a8f847f6d4fd0ddf03cc5e44bdadc7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Carbon - metabolism</topic><topic>Chlorophyll</topic><topic>Chlorophyll - metabolism</topic><topic>Kinetics</topic><topic>Life Sciences</topic><topic>Light</topic><topic>Nitrates - metabolism</topic><topic>Oxygen - metabolism</topic><topic>Photosynthesis</topic><topic>Photosynthesis - physiology</topic><topic>Photosynthesis - radiation effects</topic><topic>Physiological aspects</topic><topic>Phytoplankton - growth & development</topic><topic>Phytoplankton - metabolism</topic><topic>Phytoplankton - radiation effects</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Regular Paper</topic><topic>Remote sensing</topic><topic>Time Factors</topic><topic>Toy industry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Halsey, Kimberly H</creatorcontrib><creatorcontrib>Milligan, Allen J</creatorcontrib><creatorcontrib>Behrenfeld, Michael J</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database (ProQuest)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Photosynthesis research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Halsey, Kimberly H</au><au>Milligan, Allen J</au><au>Behrenfeld, Michael J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Physiological optimization underlies growth rate-independent chlorophyll-specific gross and net primary production</atitle><jtitle>Photosynthesis research</jtitle><stitle>Photosynth Res</stitle><addtitle>Photosynth Res</addtitle><date>2010-02-01</date><risdate>2010</risdate><volume>103</volume><issue>2</issue><spage>125</spage><epage>137</epage><pages>125-137</pages><issn>0166-8595</issn><eissn>1573-5079</eissn><notes>http://dx.doi.org/10.1007/s11120-009-9526-z</notes><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Characterization of physiological variability in phytoplankton photosynthetic efficiencies is one of the greatest challenges in assessing ocean net primary production (NPP) from remote sensing of surface chlorophyll (Chl). Nutrient limitation strongly influences phytoplankton intracellular pigmentation, but its impact on Chl-specific NPP (NPP *) is debated. We monitored six indices of photosynthetic activity in steady-state Dunaliella tertiolecta cultures over a range of nitrate-limited growth rates (μ), including photosynthetic efficiency of PSII (F v/F m), O₂-based gross and net production, 20 min and 24 h carbon assimilation, and carbon- and μ-based NPP. Across all growth rates, O₂-based Chl-specific gross primary production ( [graphic removed] ), NPP *, and F v/F m were constant. [graphic removed] was 3.3 times greater than NPP *. In stark contrast, Chl-specific short-term C fixation showed clear linear dependence on μ, reflecting differential allocation of photosynthate between short-lived C products and longer-term storage products. Indeed, ¹⁴C incorporation into carbohydrates was five times greater in cells growing at 1.2 day⁻¹ than 0.12 day⁻¹. These storage products are catabolized for ATP and reductant generation within the period of a cell cycle. The relationship between Chl-specific gross and net O₂ production, short-term ¹⁴C-uptake, NPP *, and growth rate reflects cellular-level regulation of fundamental metabolic pathways in response to nutrient limitation. We conclude that growth rate-dependent photosynthate metabolism bridges the gap between gross and net production and resolves a controversial question regarding nutrient limitation effects on primary production measures.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><pmid>20066494</pmid><doi>10.1007/s11120-009-9526-z</doi><tpages>13</tpages></addata></record> |
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subjects | Biochemistry Biomedical and Life Sciences Carbon - metabolism Chlorophyll Chlorophyll - metabolism Kinetics Life Sciences Light Nitrates - metabolism Oxygen - metabolism Photosynthesis Photosynthesis - physiology Photosynthesis - radiation effects Physiological aspects Phytoplankton - growth & development Phytoplankton - metabolism Phytoplankton - radiation effects Plant Genetics and Genomics Plant Physiology Plant Sciences Regular Paper Remote sensing Time Factors Toy industry |
title | Physiological optimization underlies growth rate-independent chlorophyll-specific gross and net primary production |
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