Photoassimilation of acetate and metabolism of carbohydrate in Chlorobium thiosulfatophilum

1. Washed cell suspensions of Chlorobium thiosulfatophilium form large amounts of a polyglucose in the light. Addition of acetate to the cells increases the formation of polysaccharide considerable. During incubation in the dark, polysaccharide decreases with time, and organic acids such as succinic...

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Published in:Archives of microbiology 1975-01, Vol.104 (2), p.105-111
Main Author: Sirev g, Reidun
Format: Article
Language:English
Subjects:
Acetates - metabolism
Bacteria - metabolism
Carbon Dioxide - metabolism
Carbon Radioisotopes
Darkness
Fermentation
Glucose - metabolism
Ketoglutaric Acids - biosynthesis
Light
Photosynthesis
Polysaccharides, Bacterial - biosynthesis
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description 1. Washed cell suspensions of Chlorobium thiosulfatophilium form large amounts of a polyglucose in the light. Addition of acetate to the cells increases the formation of polysaccharide considerable. During incubation in the dark, polysaccharide decreases with time, and organic acids such as succinic and propionic acid are excreted into the medium. 2. Glucose isolated from cells which had photoassimilated 1-, 2-, and U-14C-acetate had a specific activity which lay between 1 and 2 times that of the acetate substrates. 3. To analyse the distribution of radioactivity in the glucose units formed during photoassimilation of 14C-acetate, 2 microbial degradations, with bakers' yeast and Zymomonas mobilis respectively, were used. The results show that acetate gives rise to carbon atoms 1 plus 2 and 5 plus 6 of glucose, wheras carbon atomes 3 plus 4 are not labelled. Further, the results indicate that glucose is not formed via the reductive pentose phosphate cycle when acetate is present.
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Washed cell suspensions of Chlorobium thiosulfatophilium form large amounts of a polyglucose in the light. Addition of acetate to the cells increases the formation of polysaccharide considerable. During incubation in the dark, polysaccharide decreases with time, and organic acids such as succinic and propionic acid are excreted into the medium. 2. Glucose isolated from cells which had photoassimilated 1-, 2-, and U-14C-acetate had a specific activity which lay between 1 and 2 times that of the acetate substrates. 3. To analyse the distribution of radioactivity in the glucose units formed during photoassimilation of 14C-acetate, 2 microbial degradations, with bakers' yeast and Zymomonas mobilis respectively, were used. The results show that acetate gives rise to carbon atoms 1 plus 2 and 5 plus 6 of glucose, wheras carbon atomes 3 plus 4 are not labelled. 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Washed cell suspensions of Chlorobium thiosulfatophilium form large amounts of a polyglucose in the light. Addition of acetate to the cells increases the formation of polysaccharide considerable. During incubation in the dark, polysaccharide decreases with time, and organic acids such as succinic and propionic acid are excreted into the medium. 2. Glucose isolated from cells which had photoassimilated 1-, 2-, and U-14C-acetate had a specific activity which lay between 1 and 2 times that of the acetate substrates. 3. To analyse the distribution of radioactivity in the glucose units formed during photoassimilation of 14C-acetate, 2 microbial degradations, with bakers' yeast and Zymomonas mobilis respectively, were used. The results show that acetate gives rise to carbon atoms 1 plus 2 and 5 plus 6 of glucose, wheras carbon atomes 3 plus 4 are not labelled. 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subjects Acetates - metabolism
Bacteria - metabolism
Carbon Dioxide - metabolism
Carbon Radioisotopes
Darkness
Fermentation
Glucose - metabolism
Ketoglutaric Acids - biosynthesis
Light
Photosynthesis
Polysaccharides, Bacterial - biosynthesis
title Photoassimilation of acetate and metabolism of carbohydrate in Chlorobium thiosulfatophilum
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