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Detection of anti-tetanus toxoid antibody on modified polyacrylonitrile fibers
Accurate determination of concentration of immunoglobulin (IgG) to tetanus toxoid is important in order to evaluate the immunogenicity of tetanus toxoid vaccines, immune competence in individual patients and to measure the prevalence of immunity in populations. Surface modified polyacrylonitrile (PA...
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| Published in: | Talanta (Oxford) 2010-10, Vol.82 (5), p.1876-1883 |
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| container_end_page | 1883 |
| container_issue | 5 |
| container_start_page | 1876 |
| container_title | Talanta (Oxford) |
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| creator | Jain, Swati Chattopadhyay, Sruti Jackeray, Richa Zainul Abid, C.K.V. Kumar, Manoj Singh, Harpal |
| description | Accurate determination of concentration of immunoglobulin (IgG) to tetanus toxoid is important in order to evaluate the immunogenicity of tetanus toxoid vaccines, immune competence in individual patients and to measure the prevalence of immunity in populations. Surface modified polyacrylonitrile (PAN) fibers were evaluated as a matrix to develop highly sensitive method for the detection of anti-tetanus antibody in a sandwich ELISA format. In the proposed method tetanus toxoid immobilized on modified PAN fibers was used to detect anti-tetanus antibody (raised in horse hence represented as horse anti-tetanus toxoid or HAT-Ab) with horse raddish peroxidase enzyme conjugated with Rabbit anti-Horse IgG (RAH-HRP) as the label within 2.5
h. A sigmoidal pattern for the detection of different concentration of antibody ranging from 1.0 to 0.0001
IU
mL
−1 was validated. The immunoassay recorded a very high sensitivity as concentration as low as 0.0005
IU
mL
−1 of HAT-Ab was detected. The intra- and inter-assay precision for 3 parallel measurements of 0.01 and for 0.001
IU
mL
−1 of antibody varied from 5.4% to 11% and 5.7% to 20% respectively. PAN fibers were also used to qualitatively access the presence of different level of anti-tetanus antibody spiked in human blood. Seroepidemiological studies to measure the immunity against tetanus were conducted with twenty-five human beings belonging to various age groups using modified PAN-ELISA. The sensitivity, specificity and the reproducibility of the developed immunoassay indicate the potential application of modified PAN fibers in the field of immunodiagnostics. |
| doi_str_mv | 10.1016/j.talanta.2010.08.003 |
| format | article |
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h. A sigmoidal pattern for the detection of different concentration of antibody ranging from 1.0 to 0.0001
IU
mL
−1 was validated. The immunoassay recorded a very high sensitivity as concentration as low as 0.0005
IU
mL
−1 of HAT-Ab was detected. The intra- and inter-assay precision for 3 parallel measurements of 0.01 and for 0.001
IU
mL
−1 of antibody varied from 5.4% to 11% and 5.7% to 20% respectively. PAN fibers were also used to qualitatively access the presence of different level of anti-tetanus antibody spiked in human blood. Seroepidemiological studies to measure the immunity against tetanus were conducted with twenty-five human beings belonging to various age groups using modified PAN-ELISA. The sensitivity, specificity and the reproducibility of the developed immunoassay indicate the potential application of modified PAN fibers in the field of immunodiagnostics.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2010.08.003</identifier><identifier>PMID: 20875591</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Acrylic Resins - chemistry ; Age ; Analytical chemistry ; Animals ; Antibodies, Bacterial - blood ; Chemistry ; Enzyme-Linked Immunosorbent Assay - instrumentation ; Enzyme-Linked Immunosorbent Assay - methods ; Enzymes, Immobilized - chemistry ; Exact sciences and technology ; Horseradish Peroxidase - chemistry ; Humans ; Immunoglobulin G - blood ; Immunoglobulin G - chemistry ; Microscopy, Electron, Scanning ; Miscellaneous ; Polyacrylonitrile fiber ; Rabbits ; Reproducibility of Results ; Sandwich enzyme linked immunosorbent assay ; Sensitivity ; Surface Properties ; Tetanus toxoid ; Tetanus Toxoid - chemistry ; Tetanus Toxoid - immunology ; Toxoid immobilization</subject><ispartof>Talanta (Oxford), 2010-10, Vol.82 (5), p.1876-1883</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-4faec044ff7a9a515b24ae94be6512a69bf11823bb79db51fd2e50376bd81a693</citedby><cites>FETCH-LOGICAL-c426t-4faec044ff7a9a515b24ae94be6512a69bf11823bb79db51fd2e50376bd81a693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23307252$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20875591$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jain, Swati</creatorcontrib><creatorcontrib>Chattopadhyay, Sruti</creatorcontrib><creatorcontrib>Jackeray, Richa</creatorcontrib><creatorcontrib>Zainul Abid, C.K.V.</creatorcontrib><creatorcontrib>Kumar, Manoj</creatorcontrib><creatorcontrib>Singh, Harpal</creatorcontrib><title>Detection of anti-tetanus toxoid antibody on modified polyacrylonitrile fibers</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>Accurate determination of concentration of immunoglobulin (IgG) to tetanus toxoid is important in order to evaluate the immunogenicity of tetanus toxoid vaccines, immune competence in individual patients and to measure the prevalence of immunity in populations. Surface modified polyacrylonitrile (PAN) fibers were evaluated as a matrix to develop highly sensitive method for the detection of anti-tetanus antibody in a sandwich ELISA format. In the proposed method tetanus toxoid immobilized on modified PAN fibers was used to detect anti-tetanus antibody (raised in horse hence represented as horse anti-tetanus toxoid or HAT-Ab) with horse raddish peroxidase enzyme conjugated with Rabbit anti-Horse IgG (RAH-HRP) as the label within 2.5
h. A sigmoidal pattern for the detection of different concentration of antibody ranging from 1.0 to 0.0001
IU
mL
−1 was validated. The immunoassay recorded a very high sensitivity as concentration as low as 0.0005
IU
mL
−1 of HAT-Ab was detected. The intra- and inter-assay precision for 3 parallel measurements of 0.01 and for 0.001
IU
mL
−1 of antibody varied from 5.4% to 11% and 5.7% to 20% respectively. PAN fibers were also used to qualitatively access the presence of different level of anti-tetanus antibody spiked in human blood. Seroepidemiological studies to measure the immunity against tetanus were conducted with twenty-five human beings belonging to various age groups using modified PAN-ELISA. The sensitivity, specificity and the reproducibility of the developed immunoassay indicate the potential application of modified PAN fibers in the field of immunodiagnostics.</description><subject>Acrylic Resins - chemistry</subject><subject>Age</subject><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>Chemistry</subject><subject>Enzyme-Linked Immunosorbent Assay - instrumentation</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Exact sciences and technology</subject><subject>Horseradish Peroxidase - chemistry</subject><subject>Humans</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin G - chemistry</subject><subject>Microscopy, Electron, Scanning</subject><subject>Miscellaneous</subject><subject>Polyacrylonitrile fiber</subject><subject>Rabbits</subject><subject>Reproducibility of Results</subject><subject>Sandwich enzyme linked immunosorbent assay</subject><subject>Sensitivity</subject><subject>Surface Properties</subject><subject>Tetanus toxoid</subject><subject>Tetanus Toxoid - chemistry</subject><subject>Tetanus Toxoid - immunology</subject><subject>Toxoid immobilization</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFkUtPGzEURq2qVUmhPwE0m4rVpNf2eMazQhWltBIqG1hbflxLjibjYDtV8-_rNAGWrCx9Pvehcwk5p7CkQPuvq2XRk56LXjKoGcglAH9HFlQOvOVi4O_JoiZjO9IOTsinnFcAwDjwj-SEgRyEGOmC_P6OBW0JcW6ib2q_0BYset7mpsS_Mbj_mYlu11RkHV3wAV2zidNO27Sb4hxKChM2PhhM-Yx88HrK-Pn4npLHHzcP1z_bu_vbX9ff7lrbsb60nddooeu8H_SoBRWGdRrHzmAvKNP9aDylknFjhtEZQb1jKIAPvXGS1m9-Si4PfTcpPm0xF7UO2eJUjWDcZiWpEB1Q2b9J7j30UoyykuJA2hRzTujVJoW1TjtFQe2dq5U6Old75wqkqoZr3cVxwtas0b1UPUuuwJcjoLPVk096tiG_cpzDwASr3NWBw2ruT8Cksg04W3Qh1RspF8Mbq_wD5m6i6Q</recordid><startdate>20101015</startdate><enddate>20101015</enddate><creator>Jain, Swati</creator><creator>Chattopadhyay, Sruti</creator><creator>Jackeray, Richa</creator><creator>Zainul Abid, C.K.V.</creator><creator>Kumar, Manoj</creator><creator>Singh, Harpal</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20101015</creationdate><title>Detection of anti-tetanus toxoid antibody on modified polyacrylonitrile fibers</title><author>Jain, Swati ; Chattopadhyay, Sruti ; Jackeray, Richa ; Zainul Abid, C.K.V. ; Kumar, Manoj ; Singh, Harpal</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-4faec044ff7a9a515b24ae94be6512a69bf11823bb79db51fd2e50376bd81a693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acrylic Resins - chemistry</topic><topic>Age</topic><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Antibodies, Bacterial - blood</topic><topic>Chemistry</topic><topic>Enzyme-Linked Immunosorbent Assay - instrumentation</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Exact sciences and technology</topic><topic>Horseradish Peroxidase - chemistry</topic><topic>Humans</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulin G - chemistry</topic><topic>Microscopy, Electron, Scanning</topic><topic>Miscellaneous</topic><topic>Polyacrylonitrile fiber</topic><topic>Rabbits</topic><topic>Reproducibility of Results</topic><topic>Sandwich enzyme linked immunosorbent assay</topic><topic>Sensitivity</topic><topic>Surface Properties</topic><topic>Tetanus toxoid</topic><topic>Tetanus Toxoid - chemistry</topic><topic>Tetanus Toxoid - immunology</topic><topic>Toxoid immobilization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jain, Swati</creatorcontrib><creatorcontrib>Chattopadhyay, Sruti</creatorcontrib><creatorcontrib>Jackeray, Richa</creatorcontrib><creatorcontrib>Zainul Abid, C.K.V.</creatorcontrib><creatorcontrib>Kumar, Manoj</creatorcontrib><creatorcontrib>Singh, Harpal</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jain, Swati</au><au>Chattopadhyay, Sruti</au><au>Jackeray, Richa</au><au>Zainul Abid, C.K.V.</au><au>Kumar, Manoj</au><au>Singh, Harpal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of anti-tetanus toxoid antibody on modified polyacrylonitrile fibers</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2010-10-15</date><risdate>2010</risdate><volume>82</volume><issue>5</issue><spage>1876</spage><epage>1883</epage><pages>1876-1883</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>ObjectType-Article-2</notes><notes>ObjectType-Feature-1</notes><abstract>Accurate determination of concentration of immunoglobulin (IgG) to tetanus toxoid is important in order to evaluate the immunogenicity of tetanus toxoid vaccines, immune competence in individual patients and to measure the prevalence of immunity in populations. Surface modified polyacrylonitrile (PAN) fibers were evaluated as a matrix to develop highly sensitive method for the detection of anti-tetanus antibody in a sandwich ELISA format. In the proposed method tetanus toxoid immobilized on modified PAN fibers was used to detect anti-tetanus antibody (raised in horse hence represented as horse anti-tetanus toxoid or HAT-Ab) with horse raddish peroxidase enzyme conjugated with Rabbit anti-Horse IgG (RAH-HRP) as the label within 2.5
h. A sigmoidal pattern for the detection of different concentration of antibody ranging from 1.0 to 0.0001
IU
mL
−1 was validated. The immunoassay recorded a very high sensitivity as concentration as low as 0.0005
IU
mL
−1 of HAT-Ab was detected. The intra- and inter-assay precision for 3 parallel measurements of 0.01 and for 0.001
IU
mL
−1 of antibody varied from 5.4% to 11% and 5.7% to 20% respectively. PAN fibers were also used to qualitatively access the presence of different level of anti-tetanus antibody spiked in human blood. Seroepidemiological studies to measure the immunity against tetanus were conducted with twenty-five human beings belonging to various age groups using modified PAN-ELISA. The sensitivity, specificity and the reproducibility of the developed immunoassay indicate the potential application of modified PAN fibers in the field of immunodiagnostics.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>20875591</pmid><doi>10.1016/j.talanta.2010.08.003</doi><tpages>8</tpages></addata></record> |
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| ispartof | Talanta (Oxford), 2010-10, Vol.82 (5), p.1876-1883 |
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| subjects | Acrylic Resins - chemistry Age Analytical chemistry Animals Antibodies, Bacterial - blood Chemistry Enzyme-Linked Immunosorbent Assay - instrumentation Enzyme-Linked Immunosorbent Assay - methods Enzymes, Immobilized - chemistry Exact sciences and technology Horseradish Peroxidase - chemistry Humans Immunoglobulin G - blood Immunoglobulin G - chemistry Microscopy, Electron, Scanning Miscellaneous Polyacrylonitrile fiber Rabbits Reproducibility of Results Sandwich enzyme linked immunosorbent assay Sensitivity Surface Properties Tetanus toxoid Tetanus Toxoid - chemistry Tetanus Toxoid - immunology Toxoid immobilization |
| title | Detection of anti-tetanus toxoid antibody on modified polyacrylonitrile fibers |
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