Monoclonal Antibody to Acetylcholine Receptor: Cell Line Established from Thymus of Patient with Myasthenia Gravis

A human B cell line producing a monoclonal antibody to an antigenic determinant of acetylcholine receptors was established by cloning B cells that had been transformed in vitro by Epstein-Barr virus. The B cells were obtained from the thymus of a patient with myasthenia gravis. The antibody produced...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1982-02, Vol.215 (4535), p.995-997
Main Authors: Kamo, Isao, Furukawa, Shoei, Tada, Aiko, Mano, Yukio, Iwasaki, Yuzo, Furuse, Tsutomu, Ito, Naoki, Hayashi, Kyozo, Satoyoshi, Eijiro
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Monoclonal
B lymphocytes
B-Lymphocytes - immunology
Cell Line
Cell lines
Cell Transformation, Viral
Cholinergic receptors
Cultured cells
Epitopes
Herpesvirus 4, Human
Humans
Innervation
Lymphocytes
Monoclonal antibodies
Muscles - immunology
Muscles - innervation
Myasthenia gravis
Myasthenia Gravis - immunology
Rats
Receptors, Cholinergic - immunology
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Summary:A human B cell line producing a monoclonal antibody to an antigenic determinant of acetylcholine receptors was established by cloning B cells that had been transformed in vitro by Epstein-Barr virus. The B cells were obtained from the thymus of a patient with myasthenia gravis. The antibody produced by the cell line precipitated acetylcholine receptors from denervated and innervated rat muscle and from human muscle, but did not show detectable response to the acetylcholine receptors from the electric organs of Narke japonica. The monoclonal antibody showed identical binding patterns in innervated and denervated rat muscles. Passive transfer of the monoclonal antibody into rats induced moderate muscle weakness and electromyographic changes characteristic of myasthenia gravis.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.6297000