Transcription factor IIA of wheat and human interacts similarly with the adenovirus-2 major late promoter

Transcription factor IIA (TFIIA) is a necessary component of many RNA polymerase II transcription complexes. Assembly of the transcription complex begins when TFIIA interacts with the promoter. We have previously purified wheat germ TFIIA to homogeneity and demonstrated that it substitutes for human...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 1990-04, Vol.168 (2), p.498-505
Main Authors: Yu, Xu-Bo, Burke, Christine, Zhang, Jimin, Marchitelli, Louis J., Davis, Elizabeth A., Ackerman, Steven
Format: Article
Language:English
Subjects:
Adenoviridae - genetics
Biological and medical sciences
DNA - analysis
DNA, Viral - analysis
Fundamental and applied biological sciences. Psychology
HeLa Cells
Humans
Molecular and cellular biology
Molecular genetics
Mutation
Nucleotide Mapping
Promoter Regions, Genetic
Transcription Factors - genetics
Transcription. Transcription factor. Splicing. Rna processing
Triticum - genetics
Triticum aestivum
Citations: Items that this one cites
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Transcription factor IIA (TFIIA) is a necessary component of many RNA polymerase II transcription complexes. Assembly of the transcription complex begins when TFIIA interacts with the promoter. We have previously purified wheat germ TFIIA to homogeneity and demonstrated that it substitutes for human TFIIA in a human in vitro transcription system which utilizes the adenovirus-2 major late promoter (Ad-2 MLP). We now show, by gel retardation assays, that wheat TFIIA interacts with the Ad-2 MLP. Extensively purified human (HeLa) TFIIA interacts with the Ad-2 MLP similarly. Both wheat and human TFIIA interact with a DNA fragment comprising the minimal promoter region (−51/+32) but not with upstream or downstream regions. With both TFIIAs multiple complexes form; the fastest wheat TFIIA/DNA complex appears to be larger than the corresponding human TFIIA/DNA complex. Limited point mutation analysis of the Ad-2 MLP demonstrates that changes at −30 (TATAA region), +1, and −1 diminish TFIIA binding, but a change at −40 does not. DNA footprint analysis of this region is not definitive, but does indicate that following TFIIA binding there are changes in the pattern of hypersensitive sites.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(90)92349-5