Loading…
Targeted Disruption of Heat Shock Transcription Factor 1 Abolishes Thermotolerance and Protection against Heat-inducible Apoptosis
Heat shock transcription factor 1 (HSF1) is a member of the vertebrate HSF family that regulates stress-inducible synthesis of heat shock proteins (HSPs). Although the synthesis of the constitutively expressed and inducible members of the heat shock family of stress proteins correlates with increase...
Saved in:
Published in: | The Journal of biological chemistry 1998-03, Vol.273 (13), p.7523-7528 |
---|---|
Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Heat shock transcription factor 1 (HSF1) is a member of the vertebrate HSF family that regulates stress-inducible synthesis
of heat shock proteins (HSPs). Although the synthesis of the constitutively expressed and inducible members of the heat shock
family of stress proteins correlates with increased cellular protection, their relative contributions in acquired cellular
resistance or âthermotoleranceâ in mammalian cells is presently unknown. We report here that constitutive expression of multiple
HSPs in cultured embryonic cells was unaffected by disruption of the murine HSF1 gene. In contrast, thermotolerance was not
attainable in hsf1
(â/â) cells, and this response was required for protection against heat-induced apoptosis. We conclude that 1) constitutive and
inducibly expressed HSPs exhibit distinct physiological functions for cellular maintenance and adaptation, respectively, and
2) other mammalian HSFs or distinct evolutionarily conserved stress response pathways do not compensate for HSF1 in the physiological
response to heat shock. |
---|---|
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.273.13.7523 |