In vitro T-lymphocyte function in head and neck cancer patients

T-lymphocyte cell function was studied in vitro in peripheral blood mononuclear cells (PBMC) from 61 male patients with head and neck squamous cell carcinomas compared to 46 control patients. Patients older than 80 years or with reduced tumor-related performance status as measured by Karnofsky score...

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Bibliographic Details
Published in:European archives of oto-rhino-laryngology 1997, Vol.254 (7), p.318-322, Article 318
Main Authors: HEIMDAL, J. H, AARSTAD, H. J, AAKVAAG, A, OLOFSSON, J
Format: Article
Language:English
Subjects:
Analysis of Variance
Biological and medical sciences
Blood Proteins - analysis
Carcinoma, Squamous Cell - blood
Carcinoma, Squamous Cell - immunology
Cell Movement
Cells, Cultured
Head and Neck Neoplasms - blood
Head and Neck Neoplasms - immunology
Human Growth Hormone - blood
Humans
Hydrocortisone - blood
Interferon-gamma - immunology
Interleukins - immunology
Male
Medical sciences
Middle Aged
Neoplasm Staging
Otorhinolaryngology (head neck, general aspects and miscellaneous)
Otorhinolaryngology. Stomatology
Prolactin - blood
Retrospective Studies
T-Lymphocytes - physiology
Testosterone - blood
Thyrotropin - blood
Tumors
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Summary:T-lymphocyte cell function was studied in vitro in peripheral blood mononuclear cells (PBMC) from 61 male patients with head and neck squamous cell carcinomas compared to 46 control patients. Patients older than 80 years or with reduced tumor-related performance status as measured by Karnofsky score less than 75 were excluded. In contrast to previous similar studies, control subjects ensured a minimum stress load by sampling all patients on the day of either diagnostic or therapeutic surgery. PBMC were separated by density-gradient centrifugation and subsequently cultured with autologous sera in vitro. The mitogen concanavalin A (Con A), which stimulates all T-cell clones, was employed. Findings showed that increased Con A stimulation and PBMC proliferation occurred with PBMC from cancer patients compared to that from control patients. In contrast, no differences could be detected with respect to the stimulated supernatant level of interleukin-2, interleukin-4 or interferon-gamma between the groups. These results suggest that T-lymphocytes from PBMC are generally affected by neoplastic disease through either a supporting cell or serum factor.
ISSN:0937-4477
1434-4726
DOI:10.1007/BF02630722