Two-dimensional electrophoretic analysis of vesicular and micellar proteins of gallbladder bile

Proteins associated with lipid vesicles or mixed micelles of human gallbladder bile were separated by Sepharose-2B gel filtration chromatography followed by protein concentration and delipidation. After two-dimensional polyacrylamide gel electrophoresis and silver staining up to 59 and 471 polypepti...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 1997-07, Vol.776 (1), p.109-115
Main Authors: He, C., Fischer, S., Meyer, G., Müller, I., Jüngst, D.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Bile - chemistry
Biological and medical sciences
Cholelithiasis - metabolism
Cholesterol - chemistry
Chromatography, Gel
Digestive system
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Gallbladder - chemistry
General aspects, investigation methods
Humans
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Micelles
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Peptides - chemistry
Peptides - isolation & purification
Proteins
Proteins - chemistry
Sepharose
Silver Staining
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Proteins associated with lipid vesicles or mixed micelles of human gallbladder bile were separated by Sepharose-2B gel filtration chromatography followed by protein concentration and delipidation. After two-dimensional polyacrylamide gel electrophoresis and silver staining up to 59 and 471 polypeptide spots were counted in vesicular and micellar fractions, respectively. As major components the plasma proteins transferrin, albumin, α-fibrinogen, β-fibrinogen, γ-immunoglobulin G, immunoglobulin light chains, α-1 antitrypsin and haptoglobin α-2 chain were identified in the lipid vesicles by comparison with human protein reference maps. However, most biliary proteins including the anionic polypeptide fraction are associated with mixed micelles. The pathophysiological significance of these proteins associated with lipids needs to be investigated further.
ISSN:0021-9673
DOI:10.1016/S0021-9673(97)00560-8