Specific craniofacial cartilage dysmorphogenesis coincides with a loss of dlx gene expression in retinoic acid-treated zebrafish embryos

Treatments of zebrafish embryos with retinoic acid (RA), a substance known to cause abnormal craniofacial cartilage development in other vertebrates, result in dose- and stage-dependent losses of dlx homeobox gene expression in several regions of the embryo, Dlx expression in neural crest cells migr...

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Bibliographic Details
Published in:Mechanisms of development 1997-01, Vol.61 (1-2), p.23-36
Main Authors: Ellies, Debra L., Langille, Robert M., Martin, C. Cristofre, Akimenko, Marie-Andrée, Ekker, Marc
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Cartilage
Cartilage - embryology
Cell Differentiation
Face - abnormalities
Gene Expression Regulation, Developmental - drug effects
Genes, Homeobox
Head skeleton
Homeobox genes
Homeodomain Proteins - physiology
In Situ Hybridization
Neural creast
Neural Crest - cytology
Retinoic acid
RNA, Messenger - genetics
Time Factors
Transcription Factors
Tretinoin - pharmacology
Zebrafish
Zebrafish - embryology
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Summary:Treatments of zebrafish embryos with retinoic acid (RA), a substance known to cause abnormal craniofacial cartilage development in other vertebrates, result in dose- and stage-dependent losses of dlx homeobox gene expression in several regions of the embryo, Dlx expression in neural crest cells migrating from the hindbrain and in the visceral arch primordia is particularly sensitive to RA treatment. The strongest effects are observed when RA is administered prior to or during crest cell migration but effects can also be observed if RA is applied when the cells have entered the primordia of the arches. Losses of dlx expression correlated either with the loss of cartilage elements originating from hindbrain neural crest cells or with abnormal morphology of these elements. Cartilage elements that originate from midbrain neural crest cells, which do not express dlx genes, are less affected. Taken together with the observation that the normal patterns of visceral arch dlx expression just prior to cartilage condensation resemble the morphology of the cartilage elements that are about to differentiate, our results suggest that dlx genes are an important part of a multi-step process in the development of a subset of craniofacial cartilage elements.
ISSN:0925-4773
1872-6356
DOI:10.1016/S0925-4773(96)00616-8