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Rescue of measles virus using a replication-deficient vaccinia-T7 vector
A system which allows the reconstitution of measles virus (MV) from cloned cDNA is described. The severely host cell restricted vaccinia vector MVA-T7 expressing bacteriophage T7 RNA polymerase was used to generate full-length antigenomic MV RNA and simultaneously the mRNAs encoding the viral N, P a...
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| Published in: | Journal of virological methods 1997-02, Vol.64 (1), p.57-64 |
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| Main Authors: | , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c389t-7c8aa552691f06fd4ac3a5568d88b651ce3f2fc11ed1462e6a820d30d75ac1a03 |
| container_end_page | 64 |
| container_issue | 1 |
| container_start_page | 57 |
| container_title | Journal of virological methods |
| container_volume | 64 |
| creator | Schneider, Henriette Spielhofer, Pius Kaelin, Karin Dötsch, Christina Radecke, Frank Sutter, Gerd Billeter, Martin A. |
| description | A system which allows the reconstitution of measles virus (MV) from cloned cDNA is described. The severely host cell restricted vaccinia vector MVA-T7 expressing bacteriophage T7 RNA polymerase was used to generate full-length antigenomic MV RNA and simultaneously the mRNAs encoding the viral N, P and L proteins in order to produce replicationally and transcriptionally active nucleocapsids. The functionality of the N, P and L proteins was demonstrated first by their ability to rescue MV specific subgenomic RNAs. Assembly and budding of reconstituted MV was shown by syncytia formation and subsequently by virus isolation. The inability of MVA-T7 to produce progeny virus in most mammalian cells circumvents the necessity to separate the reconstituted MV from the MVA-T7 helper virus. Since all components are expressed transiently, this system is especially suitable for studying the functions of N, P and L. Furthermore, it is useful for investigating later steps in the MV life cycle. |
| doi_str_mv | 10.1016/S0166-0934(96)02137-4 |
| format | article |
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The severely host cell restricted vaccinia vector MVA-T7 expressing bacteriophage T7 RNA polymerase was used to generate full-length antigenomic MV RNA and simultaneously the mRNAs encoding the viral N, P and L proteins in order to produce replicationally and transcriptionally active nucleocapsids. The functionality of the N, P and L proteins was demonstrated first by their ability to rescue MV specific subgenomic RNAs. Assembly and budding of reconstituted MV was shown by syncytia formation and subsequently by virus isolation. The inability of MVA-T7 to produce progeny virus in most mammalian cells circumvents the necessity to separate the reconstituted MV from the MVA-T7 helper virus. Since all components are expressed transiently, this system is especially suitable for studying the functions of N, P and L. Furthermore, it is useful for investigating later steps in the MV life cycle.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/S0166-0934(96)02137-4</identifier><identifier>PMID: 9029530</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Bacteriophage T7 - enzymology ; Base Sequence ; Biological and medical sciences ; Cell Line ; Cercopithecus aethiops ; Defective interfering RNAs ; Defective Viruses - genetics ; DNA, Viral - analysis ; DNA-Directed RNA Polymerases - genetics ; Fundamental and applied biological sciences. Psychology ; Genetic Vectors ; Genome, Viral ; HeLa Cells ; Humans ; Measles ; Measles virus - genetics ; Measles virus - isolation & purification ; Measles virus - physiology ; Microbiology ; Molecular Sequence Data ; MVA-T7 ; Techniques used in virology ; Vaccinia virus - genetics ; Vero Cells ; Viral Proteins ; Virology ; Virus Replication</subject><ispartof>Journal of virological methods, 1997-02, Vol.64 (1), p.57-64</ispartof><rights>1997</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-7c8aa552691f06fd4ac3a5568d88b651ce3f2fc11ed1462e6a820d30d75ac1a03</citedby><cites>FETCH-LOGICAL-c389t-7c8aa552691f06fd4ac3a5568d88b651ce3f2fc11ed1462e6a820d30d75ac1a03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,783,787,27936,27937</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2557553$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9029530$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schneider, Henriette</creatorcontrib><creatorcontrib>Spielhofer, Pius</creatorcontrib><creatorcontrib>Kaelin, Karin</creatorcontrib><creatorcontrib>Dötsch, Christina</creatorcontrib><creatorcontrib>Radecke, Frank</creatorcontrib><creatorcontrib>Sutter, Gerd</creatorcontrib><creatorcontrib>Billeter, Martin A.</creatorcontrib><title>Rescue of measles virus using a replication-deficient vaccinia-T7 vector</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>A system which allows the reconstitution of measles virus (MV) from cloned cDNA is described. The severely host cell restricted vaccinia vector MVA-T7 expressing bacteriophage T7 RNA polymerase was used to generate full-length antigenomic MV RNA and simultaneously the mRNAs encoding the viral N, P and L proteins in order to produce replicationally and transcriptionally active nucleocapsids. The functionality of the N, P and L proteins was demonstrated first by their ability to rescue MV specific subgenomic RNAs. Assembly and budding of reconstituted MV was shown by syncytia formation and subsequently by virus isolation. The inability of MVA-T7 to produce progeny virus in most mammalian cells circumvents the necessity to separate the reconstituted MV from the MVA-T7 helper virus. Since all components are expressed transiently, this system is especially suitable for studying the functions of N, P and L. Furthermore, it is useful for investigating later steps in the MV life cycle.</description><subject>Animals</subject><subject>Bacteriophage T7 - enzymology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cercopithecus aethiops</subject><subject>Defective interfering RNAs</subject><subject>Defective Viruses - genetics</subject><subject>DNA, Viral - analysis</subject><subject>DNA-Directed RNA Polymerases - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Vectors</subject><subject>Genome, Viral</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Measles</subject><subject>Measles virus - genetics</subject><subject>Measles virus - isolation & purification</subject><subject>Measles virus - physiology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>MVA-T7</subject><subject>Techniques used in virology</subject><subject>Vaccinia virus - genetics</subject><subject>Vero Cells</subject><subject>Viral Proteins</subject><subject>Virology</subject><subject>Virus Replication</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFkE1LJDEQhsPioqPuTxD6IKKHXpNOJ50-iYhfICyoew5lpSKRnu4x6R7Yf296Z5irlxTJ-1RVeBg7Efy34EJfvuRDl7yV9XmrL3glZFPWP9hCmKbNz6beY4sdcsAOU_rgnKtGyn223_KqVZIv2MMzJZyoGHyxJEgdpWId4pSKKYX-vYAi0qoLCGMY-tKRDxioH4s1IIY-QPnaFGvCcYjH7KeHLtGvbT1if-9uX28eyqc_9483108lStOOZYMGQKlKt8Jz7V0NKPNdG2fMm1YCSfrKoxDkRK0r0mAq7iR3jQIUwOURO9vMXcXhc6I02mVISF0HPQ1Tso0xleF1nUG1ATEOKUXydhXDEuI_K7idDdr_Bu2sx7a5zgbt3HeyXTC9LcnturbKcn66zSEhdD5CjyHtsEqpRimZsasNRlnGOlC0aVaH5ELMwqwbwjcf-QKWe4yX</recordid><startdate>19970201</startdate><enddate>19970201</enddate><creator>Schneider, Henriette</creator><creator>Spielhofer, Pius</creator><creator>Kaelin, Karin</creator><creator>Dötsch, Christina</creator><creator>Radecke, Frank</creator><creator>Sutter, Gerd</creator><creator>Billeter, Martin A.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970201</creationdate><title>Rescue of measles virus using a replication-deficient vaccinia-T7 vector</title><author>Schneider, Henriette ; Spielhofer, Pius ; Kaelin, Karin ; Dötsch, Christina ; Radecke, Frank ; Sutter, Gerd ; Billeter, Martin A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-7c8aa552691f06fd4ac3a5568d88b651ce3f2fc11ed1462e6a820d30d75ac1a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Bacteriophage T7 - enzymology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cercopithecus aethiops</topic><topic>Defective interfering RNAs</topic><topic>Defective Viruses - genetics</topic><topic>DNA, Viral - analysis</topic><topic>DNA-Directed RNA Polymerases - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Vectors</topic><topic>Genome, Viral</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Measles</topic><topic>Measles virus - genetics</topic><topic>Measles virus - isolation & purification</topic><topic>Measles virus - physiology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>MVA-T7</topic><topic>Techniques used in virology</topic><topic>Vaccinia virus - genetics</topic><topic>Vero Cells</topic><topic>Viral Proteins</topic><topic>Virology</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schneider, Henriette</creatorcontrib><creatorcontrib>Spielhofer, Pius</creatorcontrib><creatorcontrib>Kaelin, Karin</creatorcontrib><creatorcontrib>Dötsch, Christina</creatorcontrib><creatorcontrib>Radecke, Frank</creatorcontrib><creatorcontrib>Sutter, Gerd</creatorcontrib><creatorcontrib>Billeter, Martin A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schneider, Henriette</au><au>Spielhofer, Pius</au><au>Kaelin, Karin</au><au>Dötsch, Christina</au><au>Radecke, Frank</au><au>Sutter, Gerd</au><au>Billeter, Martin A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rescue of measles virus using a replication-deficient vaccinia-T7 vector</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>1997-02-01</date><risdate>1997</risdate><volume>64</volume><issue>1</issue><spage>57</spage><epage>64</epage><pages>57-64</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>A system which allows the reconstitution of measles virus (MV) from cloned cDNA is described. The severely host cell restricted vaccinia vector MVA-T7 expressing bacteriophage T7 RNA polymerase was used to generate full-length antigenomic MV RNA and simultaneously the mRNAs encoding the viral N, P and L proteins in order to produce replicationally and transcriptionally active nucleocapsids. The functionality of the N, P and L proteins was demonstrated first by their ability to rescue MV specific subgenomic RNAs. Assembly and budding of reconstituted MV was shown by syncytia formation and subsequently by virus isolation. The inability of MVA-T7 to produce progeny virus in most mammalian cells circumvents the necessity to separate the reconstituted MV from the MVA-T7 helper virus. Since all components are expressed transiently, this system is especially suitable for studying the functions of N, P and L. Furthermore, it is useful for investigating later steps in the MV life cycle.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>9029530</pmid><doi>10.1016/S0166-0934(96)02137-4</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of virological methods, 1997-02, Vol.64 (1), p.57-64 |
| issn | 0166-0934 1879-0984 |
| language | eng |
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| source | Elsevier |
| subjects | Animals Bacteriophage T7 - enzymology Base Sequence Biological and medical sciences Cell Line Cercopithecus aethiops Defective interfering RNAs Defective Viruses - genetics DNA, Viral - analysis DNA-Directed RNA Polymerases - genetics Fundamental and applied biological sciences. Psychology Genetic Vectors Genome, Viral HeLa Cells Humans Measles Measles virus - genetics Measles virus - isolation & purification Measles virus - physiology Microbiology Molecular Sequence Data MVA-T7 Techniques used in virology Vaccinia virus - genetics Vero Cells Viral Proteins Virology Virus Replication |
| title | Rescue of measles virus using a replication-deficient vaccinia-T7 vector |
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