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Expression of mRNA for vasoactive intestinal peptide in rat small intestine
Transplantation of small intestine is a neural model that permits studies of expression of the neuropeptide, vasoactive intestinal peptide, following extrinsic denervation, transection of intrinsic neural pathways, and an ischemic interval. Tissue levels of vasoactive intestinal peptide were examine...
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| Published in: | Molecular and cellular endocrinology 1996-01, Vol.116 (1), p.31-37 |
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| container_end_page | 37 |
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| container_title | Molecular and cellular endocrinology |
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| creator | Stadelmann, A.M. Telford, G.L. Appel, D.A. Walgenbach-Telford, S. Hopp, K. Meier, D.A. Koch, T.R. |
| description | Transplantation of small intestine is a neural model that permits studies of expression of the neuropeptide, vasoactive intestinal peptide, following extrinsic denervation, transection of intrinsic neural pathways, and an ischemic interval. Tissue levels of vasoactive intestinal peptide were examined at 3 months in ileum from a sham operation, in ileum after resection of proximal small intestine, in ileum after resection of proximal small intestine and extrinsic denervation, in ileum after resection of proximal small intestine and 30 min of ischemia, and in ileum obtained 3 months after ileal isografting in Lewis-to-Lewis combinations. Vasoactive intestinal peptide levels were increased in transplanted rat ileum, resection controls, denervation controls, and ischemic controls compared to sham-operated ileum (pANOVA < 0.01). The increased levels of this peptide were highest in denervation controls and lowest in ischemic controls. Northern blot analysis using rat vasoactive intestinal peptide cDNA identified a single 1.7-kb transcript in normal and transplanted rat ileum. The density of vasoactive intestinal peptide transcripts was increased in transplanted ileum (8450 ± 540) compared to normal ileum (5790 ± 620) (
P < 0.01), and the ratio of this transcript to glyceraldehyde-3-phosphate dehydrogenase density units was also increased in transplanted ileum (0.81 ± 0.08) compared to normal ileum (0.40 ± 0.07;
P < 0.01). Enhanced transcriptional regulation was the likely mechanism for increased tissue vasoactive intestinal peptide. The increased tissue levels appeared to be a response to extrinsic denervation and transection of intrinsic neural pathways, while an ischemic interval appeared to decrease tissue levels of the peptide. |
| doi_str_mv | 10.1016/0303-7207(95)03693-8 |
| format | article |
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P < 0.01), and the ratio of this transcript to glyceraldehyde-3-phosphate dehydrogenase density units was also increased in transplanted ileum (0.81 ± 0.08) compared to normal ileum (0.40 ± 0.07;
P < 0.01). Enhanced transcriptional regulation was the likely mechanism for increased tissue vasoactive intestinal peptide. The increased tissue levels appeared to be a response to extrinsic denervation and transection of intrinsic neural pathways, while an ischemic interval appeared to decrease tissue levels of the peptide.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>DOI: 10.1016/0303-7207(95)03693-8</identifier><identifier>PMID: 8822262</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Animals ; Denervation ; Gene expression ; Gene Expression Regulation ; Intestine, Small - innervation ; Intestine, Small - metabolism ; Intestine, Small - transplantation ; Ischemia - genetics ; Ischemia - metabolism ; Radioimmunoassay ; Rat ; Rats ; Rats, Inbred Lew ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Small intestine ; Transplantation, Isogeneic ; Up-Regulation ; Vasoactive intestinal peptide ; Vasoactive Intestinal Peptide - genetics ; Vasoactive Intestinal Peptide - metabolism</subject><ispartof>Molecular and cellular endocrinology, 1996-01, Vol.116 (1), p.31-37</ispartof><rights>1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-ddb5551e36cd6e44d810851081e76e3261cd6817e45e0d1ccc2ba2baeb0029183</citedby><cites>FETCH-LOGICAL-c388t-ddb5551e36cd6e44d810851081e76e3261cd6817e45e0d1ccc2ba2baeb0029183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8822262$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stadelmann, A.M.</creatorcontrib><creatorcontrib>Telford, G.L.</creatorcontrib><creatorcontrib>Appel, D.A.</creatorcontrib><creatorcontrib>Walgenbach-Telford, S.</creatorcontrib><creatorcontrib>Hopp, K.</creatorcontrib><creatorcontrib>Meier, D.A.</creatorcontrib><creatorcontrib>Koch, T.R.</creatorcontrib><title>Expression of mRNA for vasoactive intestinal peptide in rat small intestine</title><title>Molecular and cellular endocrinology</title><addtitle>Mol Cell Endocrinol</addtitle><description>Transplantation of small intestine is a neural model that permits studies of expression of the neuropeptide, vasoactive intestinal peptide, following extrinsic denervation, transection of intrinsic neural pathways, and an ischemic interval. Tissue levels of vasoactive intestinal peptide were examined at 3 months in ileum from a sham operation, in ileum after resection of proximal small intestine, in ileum after resection of proximal small intestine and extrinsic denervation, in ileum after resection of proximal small intestine and 30 min of ischemia, and in ileum obtained 3 months after ileal isografting in Lewis-to-Lewis combinations. Vasoactive intestinal peptide levels were increased in transplanted rat ileum, resection controls, denervation controls, and ischemic controls compared to sham-operated ileum (pANOVA < 0.01). The increased levels of this peptide were highest in denervation controls and lowest in ischemic controls. Northern blot analysis using rat vasoactive intestinal peptide cDNA identified a single 1.7-kb transcript in normal and transplanted rat ileum. The density of vasoactive intestinal peptide transcripts was increased in transplanted ileum (8450 ± 540) compared to normal ileum (5790 ± 620) (
P < 0.01), and the ratio of this transcript to glyceraldehyde-3-phosphate dehydrogenase density units was also increased in transplanted ileum (0.81 ± 0.08) compared to normal ileum (0.40 ± 0.07;
P < 0.01). Enhanced transcriptional regulation was the likely mechanism for increased tissue vasoactive intestinal peptide. The increased tissue levels appeared to be a response to extrinsic denervation and transection of intrinsic neural pathways, while an ischemic interval appeared to decrease tissue levels of the peptide.</description><subject>Animals</subject><subject>Denervation</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Intestine, Small - innervation</subject><subject>Intestine, Small - metabolism</subject><subject>Intestine, Small - transplantation</subject><subject>Ischemia - genetics</subject><subject>Ischemia - metabolism</subject><subject>Radioimmunoassay</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Inbred Lew</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Small intestine</subject><subject>Transplantation, Isogeneic</subject><subject>Up-Regulation</subject><subject>Vasoactive intestinal peptide</subject><subject>Vasoactive Intestinal Peptide - genetics</subject><subject>Vasoactive Intestinal Peptide - metabolism</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqFkMlKA0EQhhtRYoy-gcKcRA-jvUwvcxFCcMOgIHpuerproGU2uydB394ZE3JUqKKg_r8WPoROCb4imIhrzDBLJcXyIueXmImcpWoPTYmSNFWYy3003VkO0VGMHxhjyamaoIlSlFJBp-jp9qsLEKNvm6Qtk_r1eZ6UbUjWJrbG9n4NiW96iL1vTJV00PXeja0kmD6JtamqnQ7H6KA0VYSTbZ2h97vbt8VDuny5f1zMl6llSvWpcwXnnAAT1gnIMqcIVnxIAlIAo4IMfUUkZBywI9ZaWpghoMCY5kSxGTrf7O1C-7kabuvaRwtVZRpoV1FLxYTkiv1rJFxmRGVyMGYbow1tjAFK3QVfm_CtCdYjbD2S1CNJnXP9C1uPj5xt96-KGtxuaEt30G82Ogw01h6CjtZDY8H5ALbXrvV_H_gBh5iNnw</recordid><startdate>19960115</startdate><enddate>19960115</enddate><creator>Stadelmann, A.M.</creator><creator>Telford, G.L.</creator><creator>Appel, D.A.</creator><creator>Walgenbach-Telford, S.</creator><creator>Hopp, K.</creator><creator>Meier, D.A.</creator><creator>Koch, T.R.</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19960115</creationdate><title>Expression of mRNA for vasoactive intestinal peptide in rat small intestine</title><author>Stadelmann, A.M. ; Telford, G.L. ; Appel, D.A. ; Walgenbach-Telford, S. ; Hopp, K. ; Meier, D.A. ; Koch, T.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-ddb5551e36cd6e44d810851081e76e3261cd6817e45e0d1ccc2ba2baeb0029183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Denervation</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Intestine, Small - innervation</topic><topic>Intestine, Small - metabolism</topic><topic>Intestine, Small - transplantation</topic><topic>Ischemia - genetics</topic><topic>Ischemia - metabolism</topic><topic>Radioimmunoassay</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Inbred Lew</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Small intestine</topic><topic>Transplantation, Isogeneic</topic><topic>Up-Regulation</topic><topic>Vasoactive intestinal peptide</topic><topic>Vasoactive Intestinal Peptide - genetics</topic><topic>Vasoactive Intestinal Peptide - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stadelmann, A.M.</creatorcontrib><creatorcontrib>Telford, G.L.</creatorcontrib><creatorcontrib>Appel, D.A.</creatorcontrib><creatorcontrib>Walgenbach-Telford, S.</creatorcontrib><creatorcontrib>Hopp, K.</creatorcontrib><creatorcontrib>Meier, D.A.</creatorcontrib><creatorcontrib>Koch, T.R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stadelmann, A.M.</au><au>Telford, G.L.</au><au>Appel, D.A.</au><au>Walgenbach-Telford, S.</au><au>Hopp, K.</au><au>Meier, D.A.</au><au>Koch, T.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of mRNA for vasoactive intestinal peptide in rat small intestine</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>1996-01-15</date><risdate>1996</risdate><volume>116</volume><issue>1</issue><spage>31</spage><epage>37</epage><pages>31-37</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-1</notes><notes>content type line 23</notes><notes>ObjectType-Article-1</notes><notes>ObjectType-Feature-2</notes><abstract>Transplantation of small intestine is a neural model that permits studies of expression of the neuropeptide, vasoactive intestinal peptide, following extrinsic denervation, transection of intrinsic neural pathways, and an ischemic interval. Tissue levels of vasoactive intestinal peptide were examined at 3 months in ileum from a sham operation, in ileum after resection of proximal small intestine, in ileum after resection of proximal small intestine and extrinsic denervation, in ileum after resection of proximal small intestine and 30 min of ischemia, and in ileum obtained 3 months after ileal isografting in Lewis-to-Lewis combinations. Vasoactive intestinal peptide levels were increased in transplanted rat ileum, resection controls, denervation controls, and ischemic controls compared to sham-operated ileum (pANOVA < 0.01). The increased levels of this peptide were highest in denervation controls and lowest in ischemic controls. Northern blot analysis using rat vasoactive intestinal peptide cDNA identified a single 1.7-kb transcript in normal and transplanted rat ileum. The density of vasoactive intestinal peptide transcripts was increased in transplanted ileum (8450 ± 540) compared to normal ileum (5790 ± 620) (
P < 0.01), and the ratio of this transcript to glyceraldehyde-3-phosphate dehydrogenase density units was also increased in transplanted ileum (0.81 ± 0.08) compared to normal ileum (0.40 ± 0.07;
P < 0.01). Enhanced transcriptional regulation was the likely mechanism for increased tissue vasoactive intestinal peptide. The increased tissue levels appeared to be a response to extrinsic denervation and transection of intrinsic neural pathways, while an ischemic interval appeared to decrease tissue levels of the peptide.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>8822262</pmid><doi>10.1016/0303-7207(95)03693-8</doi><tpages>7</tpages></addata></record> |
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| ispartof | Molecular and cellular endocrinology, 1996-01, Vol.116 (1), p.31-37 |
| issn | 0303-7207 1872-8057 |
| language | eng |
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| subjects | Animals Denervation Gene expression Gene Expression Regulation Intestine, Small - innervation Intestine, Small - metabolism Intestine, Small - transplantation Ischemia - genetics Ischemia - metabolism Radioimmunoassay Rat Rats Rats, Inbred Lew RNA, Messenger - genetics RNA, Messenger - metabolism Small intestine Transplantation, Isogeneic Up-Regulation Vasoactive intestinal peptide Vasoactive Intestinal Peptide - genetics Vasoactive Intestinal Peptide - metabolism |
| title | Expression of mRNA for vasoactive intestinal peptide in rat small intestine |
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